TiO supported single Ag atoms nanozyme for elimination of SARS-CoV2.

The outbreak of SARS-coronavirus 2 (SARS-CoV2) has become a global health emergency. Although enormous efforts have been made, there is still no effective treatment against the new virus. Herein, a TiO supported single-atom nanozyme containing atomically dispersed Ag atoms (Ag-TiO SAN) is designed to serve as a highly efficient antiviral nanomaterial.

[Analysis of AVPR2 variant in a neonate with congenital nephrogenic diabetes insipidus].

Objective: To detect potential variant in a male neonate affected with congenital nephrogenic diabetes insipidus (CNDI).

Methods: Clinical data of the patient was collected. Genomic DNA was extracted from peripheral blood samples from the child and his parents.

A 10-year retrospective study of neonatal lupus erythematous in China.

Background: Neonatal lupus erythematosus (NLE) is not a common disease. The death rate of complete congenital heart block (CCHB), which is the most severe clinical manifestation, is as high as 20% to 30%, so early recognition of infants at risk is important.

Objectives: To investigate the clinical features and long-term prognosis of NLE.

H-ferritin-nanocaged doxorubicin nanoparticles specifically target and kill tumors with a single-dose injection.

An ideal nanocarrier for efficient drug delivery must be able to target specific cells and carry high doses of therapeutic drugs and should also exhibit optimized physicochemical properties and biocompatibility. However, it is a tremendous challenge to engineer all of the above characteristics into a single carrier particle. Here, we show that natural H-ferritin (HFn) nanocages can carry high doses of doxorubicin (Dox) for tumor-specific targeting and killing without any targeting ligand functionalization or property modulation.

A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1.

Disturbances in the normal functions of the endoplasmic reticulum (ER) can lead to the accumulation of unfolded proteins and disturbance of Ca(2+) regulation within the lumen of ER, and arouse a series of complicated response termed unfolded protein response (UPR), which is aimed initially at reestablishing homeostasis and normal physiology but can ultimately trigger cell death if the UPR fails to compensate for damage. Here we show that ER locating human RING finger E3 ligase RNF186 participates in the process of ER stress-mediated apoptosis. Overexpression of RNF186 stimulates upregulation of ER sensor proteins and rapid transmission of ER Ca(2+) in Hela cells, while RNF186 knockdown exhibits a moderate degree of resistance to ER stress, indicating RNF186 can arouse stress signaling at ER.

Risk factors and clinical analysis for invasive fungal infection in neonatal intensive care unit patients.

Objective: In this study, we seek to determine independent risk factors of invasive fungal infection (IFI) in neonatal infants.

Study Design: The medical charts of 5135 neonatal intensive care unit admissions in the past 7 years between January 2004 and December 2010 were reviewed and 45 neonates were found with IFI. Two controls, matched by gestational age, birth weight category, admission date, ward, hospital stay, and admission age, were selected for each case.

RNF152, a novel lysosome localized E3 ligase with pro-apoptotic activities.

RING finger protein 152 (RNF152) is a novel RING finger protein and has not been well characterized. We report here that RNF152 is a canonical RING finger protein and has E3 ligase activity. It is polyubiqitinated partly through Lys-48-linked ubiquitin chains in vivo and this phenomenon is dependent on its RING finger domain and transmembrane domain.

[Clinical characteristics and outcomes of respiratory distress syndrome in term and late-preterm neonates].

Objective: Respiratory distress syndrome (RDS) is a frequently seen acute respiratory disorder in the newborn infants. Since the original description of deficiency of the pulmonary surfactant in premature neonates by Avery in 1959, RDS has most commonly been attributed to developmental immaturity of surfactant production. But in clinical practice it has been found that there was RDS in term and late-term neonates.

[Association between clinical manifestations of infants with human cytomegalovirus infection and glycoprotein B genotype].

Objective: To investigate the association between the clinical manifestations of infants with human cytomegalovirus (HCMV) infection and glycoprotein B (gB) genotype.

Methods: Urine samples were obtained from 107 symptomatic infants with HCMV infection confirmed by fluorescence quantitative PCR, 70 male and 37 female, aged 5 d-8 months, and 25 asymptomatic infants with HCMV infection, 16 male and 9 female, aged 21 d-7 months. A fragment of glycoprotein B gene was amplified by nested PCR (nPCR).

[Effects of phytoestrogens on gap junctional intercellular communication].

Objective: To observe effects of phytoestrogens quercetin (QC), Genistein (GEN), coumestrol (COM), and enterolactone (ENL) on gap junctional intercellular communication (GJIC) in HaCaT cells.

Methods: HaCaT cells were exposed to QC, GEN, COM, and ENL at 0.1, 1.

[Application of flow cytometry to detect PP65 antigenemia for diagnosis and monitoring of human cytomegalovirus infection].

Objectives: To evaluate the clinical significance of flow cytometry (FCM) to detect the cytomegalovirus (CMV) PP65 antigen in patients with CMV infection.

Methods: Samples from 35 patients without CMV infection were used as negative control. The definite diagnosis of CMV infection was based on the national criteria for CMV infection.

[Relationship among serum cortisol and insulin and blood glucose in the asphyxiated neonates].

Objective: To explore the dynamic change of serum cortisol and insulin levels, and their relation with blood glucose concentration in asphyxiated neonates.

Methods: The levels of serum cortisol and insulin at d1,d3 and d7 of birth were measured by radioimmunoassay and the concentration of blood glucose was measured with glucose oxidase method in 43 asphyxiated neonates.

Results: The levels of serum cortisol at d 1, d 3 and d 7 of birth were gradually decreased (P<0.