Publications by authors named "Jiya Eerdeng"
Article Synopsis
- Aging affects our immune system because of changes in stem cells that regenerate immune cells.
- In a study comparing mice with different aging phenotypes, researchers found that certain stem cells in early aging mice showed increased aging-related gene activity, while those in delayed aging mice had genes helping with regulation and external responses.
- The shifts in blood cell lineage biases among hematopoietic stem cells (HSCs) reveal that targeting specific HSC subsets could be key in developing strategies to delay aging and improve immune function.
Article Synopsis
- * In a study using a Tet2 knockout mouse model, only a small number of hematopoietic stem cells substantially expanded after Tet2 was knocked out, and these cells showed lower expression of leukemia-related genes.
- * The research found that knocking down an RNA splicing factor, Rbm25, accelerated the growth of Tet2-deficient cells, suggesting that variations in RNA splicing may influence the risk of developing preleukemic conditions.
Cellular heterogeneity is a major cause of treatment resistance in cancer. Despite recent advances in single-cell genomic and transcriptomic sequencing, it remains difficult to relate measured molecular profiles to the cellular activities underlying cancer. Here, we present an integrated experimental system that connects single cell gene expression to heterogeneous cancer cell growth, metastasis, and treatment response.
View Article and Find Full Text PDFEmbedded viral barcoding in combination with high-throughput sequencing is a powerful technology with which to track single-cell clones. It can provide clonal-level insights into cellular proliferation, development, differentiation, migration, and treatment efficacy. Here, we present a detailed protocol for a viral barcoding procedure that includes the creation of barcode libraries, the viral delivery of barcodes, the recovery of barcodes, and the computational analysis of barcode sequencing data.
View Article and Find Full Text PDFIn most organ systems, regeneration is a coordinated effort that involves many stem cells, but little is known about whether and how individual stem cells compensate for the differentiation deficiencies of other stem cells. Functional compensation is critically important during disease progression and treatment. Here, we show how individual hematopoietic stem cell (HSC) clones heterogeneously compensate for the lymphopoietic deficiencies of other HSCs in a mouse.
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