Insights into the combined toxicity and mechanisms of BDE-47 and PFOA in marine blue mussel: An integrated study at the physiochemical and molecular levels.

The coexistence of multiple emerging contaminants imposes a substantial burden on the ecophysiological functions in organisms. The combined toxicity and underlying mechanism requires in-depth understanding. Here, marine blue mussel (Mytilus galloprovincialis L.

Phenylhydrazone Alkaloids from the Deep-Sea Cold Seep Derived Fungus HDN21-0307.

Alkaloids with a phenylhydrazone architecture are rarely found in nature. Four unusual phenylhydrazone alkaloids named talarohydrazones A-D (-) were isolated from the deep-sea cold seep derived fungus HDN21-0307 using the one strain-many compounds (OSMAC) approach and MS/MS-based molecular networking (MN) combined with network annotation propagation (NAP) and the unsupervised substructure annotation method MS2LDA. Their structures were elucidated by spectroscopic data analysis, single-crystal X-ray diffraction, and quantum chemical calculations.

Metabolites and metabolic pathway analysis of sulfadimidine in carp (Cyprinus carpio) based on UHPLC-Q-orbitrap HRMS.

Sulfadimidine (SM) is an N-substituted derivative of p-aminobenzenesulfonyl structure. This study aimed to analyze the metabolism of SM in carp (Cyprinus carpio). The carps were fed with SM at a dose of 200 mg/(kg · bw) and then killed.

Integrative Inducer Intervention and Transcriptomic Analyses Reveal the Metabolism of Paralytic Shellfish Toxins in .

Paralytic shellfish toxins (PSTs) are widely distributed neurotoxins, and the PST metabolic detoxification mechanism in bivalves has received increasing attention. To reveal the effect of phase I (cytochrome P450)-II (GST)-III (ABC transport) metabolic systems on the PST metabolism in , this study amplified stress on the target systems using rifampicin, dl-α-tocopherol, and colchicine; measured PST levels; and conducted transcriptomic analyses. The highest toxin content reached 1623.

A novel ZIF-L/PEI thin film nanocomposite membrane for removing perfluoroalkyl substances (PFASs) from water: Enhanced retention and high flux.

Membrane separation technology is widely recognized as an effective method for removing perfluoroalkyl substances (PFASs) in water treatment. ZIF-L, a metal-organic framework (MOF) family characterized by its mat-like cavities and leaf-like morphology, has garnered considerable interest and has been extensively employed in fabricating thin-film nanocomposite (TFN) membranes. In this study, a robust, high-performance TFN membrane to remove PFASs in a nanofiltration (NF) process was created through an interfacial polymerization approach on the surface of polysulfone (PSF), incorporating ZIF-L within the selective layer.

Contamination Status and Risk Assessment of Paralytic Shellfish Toxins in Shellfish along the Coastal Areas of China.

Paralytic shellfish toxins (PSTs) are widely distributed in shellfish along the coast of China, causing a serious threat to consumer health; however, there is still a lack of large-scale systematic investigations and risk assessments. Herein, 641 shellfish samples were collected from March to November 2020, and the PSTs' toxicity was detected via liquid chromatography-tandem mass spectrometry. Furthermore, the contamination status and potential dietary risks of PSTs were discussed.

Characteristic Profile of the Hazardous, Nutritional, and Taste-Contributing Compounds during the Growth of with Different Shell Colors.

Bay scallops () are shellfish with high nutritional and economic value. However, nutritional studies on with different shell colors are limited. This study examines the hazardous, nutritional, and taste-contributing compounds during the growth of with different shell colors.

Risk characteristics of shellfish toxins in Mytilus unguiculatus around the Zhoushan Islands, East China Sea.

The Zhoushan Islands, are an important area for Mytilus unguiculatus aquaculture, and are threatened by potentially harmful algal blooms. However, a full understanding of the risks posed by their toxin residues is still lacking. M.

Adaptive responses of geographically distinct strains of the benthic dinoflagellate, Prorocentrum lima (Dinophyceae), to varying light intensity and photoperiod.

The toxic Prorocentrum lima complex can potentially cause serious harm to the benthos and entire food chain. Studies have revealed physiological differences in strains from different regions related to local environment, while differences in the adaptive responses of P. lima complex should be urgently assessed.

Variation and characterization of prometryn in oysters (Crassostrea gigas) after seawater exposure.

Prometryn (PRO) is frequently detected in shellfish of international trade among triazine herbicides because of its wide application in agriculture and aquaculture worldwide. Nevertheless, the variations of PRO remain unclear in aquatic organisms, which affect the accuracy of their food safety risk assessment. In the present study, the tissue-specific accumulation, biotransformation, and potential metabolic pathway of PRO were reported in oyster species Crassostrea gigas for the first time.

Perfluoroalkyl substances (PFASs) in aquatic products from the Yellow-Bohai Sea coasts, China: Concentrations and profiles across species and regions.

Perfluoroalkyl substances (PFASs) are emerging contaminants capable of harming human health, primarily via ingesting aquatic products. The current study monitored a survey of 23 PFASs in 1049 aquatic products from the coasts of the Yellow-Bohai Sea in China to comprehensively investigate the concentrations and distributions of PFASs. PFOA, PFOS, PFNA, PFOSA, and PFUdA were more predominantly and frequently detected than other PFASs in all samples, dominating PFAS patterns in aquatic products.

Residue Analysis and Assessment of the Risk of Dietary Exposure to Domoic Acid in Shellfish from the Coastal Areas of China.

Harmful algal blooms in Chinese waters have caused serious domoic acid (DA) contamination in shellfish. Although shellfish are at particular risk of dietary exposure to DA, there have been no systematic DA risk assessments in Chinese coastal waters. A total of 451 shellfish samples were collected from March to November 2020.

Seasonal variations of heavy metals in seawater and integrated poly-cultured scallop Chlamys farreri in Ailian Bay, northern China.

Seasonal variations of heavy metals in integrated poly-cultured scallops and seawater from Ailian Bay, northern China were analyzed to reveal the potential factor in bioaccumulation of metals in scallop Chlamys farreri. Results showed that heavy metals (Cu, Zn, As, Cd, Cr, Pb and Hg) in seawater were much below the maximum permissible limits and showed no seasonal changes, but were consistent with the growing period of the poly-cultivated kelp. The content of Zn in scallop tissues was highest with an average value of 88.

Transcriptome Analysis Reveals MAPK/AMPK as a Key Regulator of the Inflammatory Response in PST Detoxification in and .

Paralytic shellfish toxins (PSTs) are an increasingly important source of pollution. Bivalves, as the main transmission medium, accumulate and metabolize PSTs while protecting themselves from damage. At present, the resistance mechanism of bivalves to PSTs is unclear.

Dimeric Tetracenomycin Derivatives from a Taklamakan Desert-Derived sp. HDN154193.

Bitetracenomycin A () and its diastereomers [(±)-bitetracenomycin B, (±)-] were discovered from the cultures of sp. HDN154193. Compounds and (±)- were the first tetracenomycin dimers obtained from a natural source with sp methine protons at the bridge positions (C-12/12'), which also exhibited broad-spectrum antibacterial activity.

Effects of single and combined exposure to BDE-47 and PFOA on distribution, bioaccumulation, and toxicity in blue mussel (Mytilus galloprovincialis).

The contamination of 2,2',4,4'-Tetrabrmodiphenyl ether (BDE-47) and perfluorooctanoic acid (PFOA) has drawn a worldwide attention over the risks in ecological and food safety. In this work, blue mussel (Mytilus galloprpvincialis) was employed to investigate the combined effects of BDE-47 (10 ng mL) and PFOA (100 ng mL) on tissue distribution, accumulation, elimination, and toxicity. Results suggested that BDE-47 and PFOA accumulated mostly in digestive gland, followed by gills and gonad, and M.

Simultaneous determination of triazine herbicides and their metabolites in shellfish by HPLC-MS/MS combined with Q/E-Orbitrap HRMS.

Triazine herbicides are used extensively in agriculture and aquaculture worldwide because of their broad effectiveness in weed control. However, after they are discharged into the sea, they seriously contaminate aquatic ecosystems and threaten aquatic organisms, especially shellfish. Currently, there are no established methods for the detection and confirmation of triazine herbicides and their metabolites in biological matrixes.

Semicarbazide Accumulation, Distribution and Chemical Forms in Scallop () after Seawater Exposure.

Semicarbazide is a newly recognized marine pollutant and has the potential to threaten marine shellfish, the ecological equilibrium and human health. In this study, we examined the accumulation, distribution, and chemical forms of semicarbazide in scallop tissues after exposure to 10, 100, and 1000 μg/L for 30 d at 10 °C. We found a positive correlation between semicarbazide residues in the scallops and the exposure concentration ( < 0.

Antibacterial -Terphenyl with a Rare 2,2'-Bithiazole Substructure and Related Compounds Isolated from the Marine-Derived Actinomycete sp. HDN154086.

Assisted by MS/MS-based molecular networking and X-ray diffraction analysis, five new -terphenyl derivatives, namely, nocarterphenyls D-H (-), were obtained and characterized from the cultures of the marine sediment-derived actinomycete sp. HDN154086. The skeleton of nocarterphenyl D () was defined to possess a rare 2,2'-bithiazole scaffold, naturally occurring for the first time, and nocarterphenyls E-H (-) are -terphenylquinones with unusual thioether linked fatty acid methyl ester substitutions.

Nontarget Screening and Toxicity Evaluation of Diol Esters of Okadaic Acid and Dinophysistoxins Reveal Intraspecies Difference of .

High-resolution mass spectrometry (HRMS) analysis with the assistance of molecular networking was used to investigate intracellular toxin profiles of five () strains sampled from the north Yellow Sea and South China Sea. Mice were used as a model species for testing the acute toxicity of intracellular okadaic acid (OA) and dinophysistoxins (DTXs) in free and esterified states. Results showed that OA and DTX1 esterified derivatives were detected in all samples, accounting for 55%-96% of total toxins in five strains.

[Pollution Characteristics of Perfluorinated Alkyl Substances (PFASs) in Seawater, Sediments, and Biological Samples from Jiaozhou Bay, China].

In order to explore the pollution levels and characteristics of perfluorinated alkyl substances (PFASs), seawater, sediment, and samples were collected near the Jiaozhou Bay coast in April 2018. All samples were analyzed by using the high performance liquid chromatography-mass spectrometry method to determine the content of 35 types of PFASs. The results showed that 12 different PFASs were tested in the seawater with ∑PFASs concentrations of 21.

Secondary Metabolites Produced by Combined Culture of and a sp.

Four new alkyl aromatics, penixylarins A-D (-), along with the known biogenetically related 1,3-dihydroxy-5-(12-hydroxyheptadecyl)benzene () and 1,3-dihydroxy-5-(12-sulfoxyheptadecyl)benzene (), were isolated from a mixed culture of the Antarctic deep-sea-derived fungus PRB-2 and the mangrove-derived fungus sp. HDN13-249. UPLC-MS data and an analysis of structural features showed that compounds and were produced by collaboration of the two fungi, while compounds - could be produced by sp.

Anthraquinone Derivatives from a Marine-Derived Fungus HDN16-802.

Five new anthraquinone derivatives, auxarthrols D-H (-), along with two known analogues (-), were obtained from the culture of the marine-derived fungus . Their structures, including absolute configurations, were established on the basis of NMR, HRESIMS, and circular dichroism (CD) spectroscopic techniques. Among them, compound represents the second isolated anthraquinone derivative with a chlorine atom, which, with compound , are the first reported anthraquinone derivatives with anticoagulant activity.

New Glutamine-Containing Azaphilone Alkaloids from Deep-Sea-Derived Fungus HDN151398.

Three new azaphilone alkaloids containing glutamine residues, namely -glutarylchaetoviridins A-C (-), together with two related compounds ( and ) were isolated from the extract of HDN151398, a fungus isolated from a deep-sea sediment sample collected in South China Sea. Their structures were elucidated on the basis of extensive 1D and 2D NMR as well as HRESIMS spectroscopic data and chemical analysis. -glutarylchaetoviridins A-C (-) represent the first class of chaetoviridins characterized by embedded glutamate residues.