Grid-based analysis of three-dimensional choroidal Sattler's and Haller's layer vascularity volume index and its relation to age and axial length in adults by ultra-widefield swept-source optical coherence tomography angiography.

Background: This study was to evaluate the features of choroidal Sattler's and Haller's layer (CSHL) vessels in adults and explore its relationship with factors such as age, axial length (AL) and body mass index (BMI) by using ultra-widefield swept-source optical coherence tomography angiography (ultra-widefield SS-OCTA).

Methods: 24mm × 20mm fovea centered rectangular SS-OCTA scans were acquired. They were divided into 9 identical grids, with each equal size of 8 mm × 6.

Comparative study between swept-source and spectral-domain OCTA for imaging of choroidal neovascularization in age-related macular degeneration.

Aim: To compare the differences of choroidal neovascularization (CNV) measurements between swept-source and spectral-domain optical coherence tomography angiography (SS-OCTA and SD-OCTA) in neovascular age-related macular degeneration (nAMD) and the imaging reliability of the two devices.

Methods: Prospective comparative study. SS-OCTA and SD-OCTA were used to scan the same eye with the modes of 3×3 and 6×6 mm centered on the neovascularization.

Research on the coupling and coordinated development of Guangxi's tourism industry, new urbanization and environmental health system in the post-epidemic era.

As one of China's sunrise industries, tourism has always been the engine to promote the development of the national economy, and in 2018, the annual income of China's tourism industry exceeded 5 trillion yuan unprecedentedly. In recent years, the traditional extensive production mode has inevitably brought about problems such as environmental pollution and public health threats, while helping the development of new urbanization, thus triggering a series of challenges in the environmental health system. The tourism industry, new urbanization, and environmental health system three cooperate and promote each other, the coordinated development between them for economic growth, new urbanization development, environmental protection, and public health play a vital role, in the post-epidemic era is a special period of historical opportunities, the public's focus from the original sacrifice of environmental health in exchange for economic growth model began to green, low-carbon sustainable development mode, Guangxi Zhuang Autonomous Region as a tourism resource endowment rich region, It is of positive significance to explore the coupling degree and coordination between the tourism industry, new urbanization and environmental health system, and put forward targeted practical enlightenment, which is of positive significance for promoting the sustainable development of tourism industry.

Single-crystalline Mg-substituted NaMn(PO)PO nanoparticles as a high capacity and superior cycling cathode for sodium-ion batteries.

Mn-based mixed phosphate NaMn(PO)(PO) (NMPP) is a promising cathode for high-potential, low-cost and eco-friendly sodium-ion batteries. However, this material still faces some bottleneck issues in terms of low conductivity, disturbance of impure crystalline phase, micron-sized agglomerated particles and the Mn Jahn-Teller effect. Herein, a Mg-substituted NMPP (NMMgPP)@C composite was constructed modified solution combustion and subsequent calcination treatment.

Synthesis and characterization of magnetic organic montmorillonite: efficient adsorption of hexavalent chromium.

This research compared two potential adsorbents for the efficient adsorption of toxic hexavalent chromium. The non-magnetic material STAC-Mt and the magnetic material FeSO-STAC-Mt were synthesized by a simple impregnation method using montmorillonite (Mt), octadearyl dimethyl ammonium chloride (STAC) and ferrous sulfate as raw materials. The structural and morphological characteristics of both adsorbents were investigated by BET, XRD, FTIR, Zeta, VSM, TEM, SEM and XPS techniques.

Effectiveness of peripheral defocus spectacle lenses in myopia control: a Meta-analysis and systematic review.

Aim: To evaluate the effectiveness of peripheral defocus spectacle lenses (PDLs) in myopia control.

Methods: Literature retrieval on PubMed, Cochrane Library, Embase, and Web of Science databases, and the search time limit was from the establishment of each database to December 29, 2021 were conducted. Change of spherical equivalent refraction (SER) and axial change (AL) were extracted from the literatures that met the inclusion criteria, and RevMan5.

Comparison of intravitreal aflibercept and dexamethasone implant in the treatment of macular edema associated with diabetic retinopathy or retinal vein occlusion: a Meta-analysis and systematic review.

Aim: To compare the efficacy and safety of intravitreal aflibercept with dexamethasone implant in the treatment of macular edema (ME) associated with diabetic retinopathy (DR) or retinal vein occlusion (RVO).

Methods: A comprehensive search of studies comparing dexamethasone and aflibercept in patients with ME was conducted at PubMed, Embase, and Cochrane Central Register of Controlled Trials from the beginning of library to April 16, 2021. Extracting the data including best-corrected visual acuity (BCVA), central retinal thickness (CRT), number of injections and serious adverse events (SAEs) from the final qualified articles.

The microbiomic and environmental analysis of sediments in the Indo-Pacific humpback dolphin (Sousa chinensis) habitat in the Northern Beibu Gulf, China.

The northern Beibu Gulf is one of the major habitats for the Indo-Pacific humpback dolphin (Sousa chinensis) in China. In this habitat, the core distribution zone of humpback dolphins was confined to the Sanniang Bay (SNB) and Dafengjiang River Estuary (DRE) areas. In our present research, the sediments of 14 sampling sites across the SNB and DRE waters were collected and further conducted for microbiomic and environmental analysis to explore the ecosystem characteristics of major humpback dolphin habitats in Northern Beibu Gulf.

hElp3 directly modulates the expression of HSP70 gene in HeLa cells via HAT activity.

Human Elongator complex, which plays a key role in transcript elongation in vitro assay, is incredibly similar in either components or function to its yeast counterpart. However, there are only a few studies focusing on its target gene characterization in vivo. We studied the effect of down-regulation of the human elongation protein 3 (hELP3) on the expression of HSP70 through antisense strategy.

Activation of formyl peptide receptor-1 enhances restitution of human retinal pigment epithelial cell monolayer under electric fields.

Purpose: This study aimed at identifying the expression of functional formyl peptide receptor (FPR)-1 in human retinal pigment epithelium (hRPE) cells and to evaluate the role of FPR in regulation of wound closure of the hRPE monolayer under electric fields (EFs).

Methods: The expression of FPR in hRPE cells was analyzed with an immunofluoresence labeling assay and RT-PCR. Cultured wounded hRPE monolayers were exposed to EFs with free serum, 20%, serum, and a classical FPR agonist, N-formyl-Met-Leu-Phe (fMLF), respectively, for 3 hours.

Inhibition of migration but stimulation of proliferation of human retinal pigment epithelial cells cultured with uniform vesicles of silicone oil.

Background: It has been hypothesized that emulsification of silicone oil might stimulate preretinal membrane formation and proliferative vitreoretinopathy. This study aimed to test the effects of vesicles of silicone oil (VSO), which were prepared by a novel membrane emulsification technique, on the migration and proliferation of human retinal pigment epithelial (hRPE) cells in vitro.

Methods: VSO were produced by extrusion of the oil through a Supor membrane (Pall Life Sciences, Port Washington, NY, USA) with 0.

[Cytoskeleton changes of cultured human retinal pigment epithelial cells in a mechanical stress model].

Objective: To observe changes of the cytoskeleton of cultured human retinal pigment epithelium (hRPE) cells in a mechanical stress model in vitro.

Methods: Ferric oxide beads, coated with collagen, were added to dishes containing substrate-attached hRPE cells. After incubation, the cells were washed to remove unbound beads.

[Effects of mechanical stress on expressions of monocyte chemoattractant protein-1 and interleukin-8 of cultured human retinal pigment epithelial cells].

Objective: To investigate the expressions of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) of cultured human retinal pigment epithelial (hRPE) cells under mechanical stress in vitro, so as to mimic and understand the role of these 2 inflammatory cytokines in the early stage of development of primary retinal detachment.

Methods: Ferric oxide beads coated with collagen were added to the culture plate containing wall-attaching hRPE cells and then the cells were incubated and washed to remove the unbound beads. A magnet was put to the culture plate to provide vertical magnetic force.

[Effects of Schwann cell-derived neurotrophic activity on cultured retinal ganglion cells survive and grow in normal and gas-deprived environment].

Purpose: To investigate the effects of Schwann cell-derived neurotrophic activity (SCNA) on cultured retinal ganglion cells (RGC) survive and the expression of growth associated protein 43 (GAP43) in normal and gas-deprived environment.

Methods: Schwann cells of new born SD rat were cultured, and remaded it into solutions containing different concentrations of SCNA. Then these solutions were added to the primarily cultured retinal cells, the activity of SCNA was determined by MTT method.

[Expression of adrenomedullin and adrenomedullin receptor in the epiretinal membranes of proliferative vitreoretinopathy].

Objective: To investigate the expression of adrenomedullin (ADM) and ADM receptor (ADMR) in epiretinal membranes (ERM) of proliferative vitreoretinopathy (PVR).

Methods: The expression of ADM and ADMR in 10 ERM were studied by in situ hybridization analysis (ISH).

Results: ADM and ADMR mRNAs were expressed in 7 of 10 specimens.

Influence of IL-1 beta and TNF-alpha on Fas expression of human retinal pigment epithelial cells in vitro.

Purpose: To observe Fas expression change of cultured human retinal pigment epithelial (RPE) cells by IL-1 beta and TNF-alpha.

Methods: With flow cytometry, immunohischemistry, and color imaging system, Fas expressions by exposure to IL-1 beta and/or TNF-alpha were measured.

Results: The gray degree values of Fas expression were 67.

Adrenomedullin affects two signal transduction pathways and the migration in retinal pigment epithelial cells.

Purpose: Adrenomedullin is a multifunctional regulatory peptide known to inhibit the migration of smooth muscle cells. In vitro studies were performed to identify whether adrenomedullin (ADM) also inhibits the migration of RPE cells. The aberrant behavior of these cells is an early event in proliferative vitreoretinopathy, and these studies were designed to determine how ADM acts on RPE cells at the second-messenger level.

[Expression of TGF-beta receptor II in periretinal membranes of proliferative vitreoretinopathy].

Purpose: To investigate the expression of transforming growth factor-beta receptor II (TGF-beta R II) in periretinal membranes of proliferative vitreoretinopathy (PVR).

Methods: Immunohistochemistry and in situ hybridization were used to detect the expression of TGF-beta R II protein and mRNA in sixteen proliferative membranes of thirteen PVR patients.

Results: Immunohistochemically staining showed that membranes labeled negative "-", weak positive "+", positive "2+" and strong positive staining "3+" were respectively noted one, one, three and four membranes in nine C2-C3 membranes.

[Inhibition of IL-6 antisense oligonucleotide on IL-6 expression by human retinal pigment epithelium in vitro].

Objective: To investigate the inhibition of IL-6 antisense oligonucleotide (ASON) on IL-6 expression by retinal pigment epithelium (RPE) cells on the basis of previous study.

Methods: Cultured human RPE cells was treated with IL-1 beta and IL-6 ASON. IL-6 mRNA and protein expression were detected by enzyme linked immunosorbent assay (ELISA), immunohistochemistry and in situ hybridization histochemistry (ISH).