Association of Radical Chemistry with LanD Flavoprotein Activity for C-Terminal Macrocyclization of a Ribosomal Peptide by Formation of an Unsaturated Thioether Residue.

LanD flavoproteins catalyze oxidative decarboxylation of the C-terminal Cys residue of a peptide to produce an enethiol. This enethiol is highly reactive and can be coupled with an upstream dehydroamino acid through Michael addition to form S-[2-aminovinyl](3-methyl)cysteine, an unsaturated thioether residue known to be characteristic of an array of C-terminally macrocyclized, ribosomally synthesized and posttranslationally modified peptides (RiPPs). Based on a two-stage bioinformatics mining of posttranslational modifications (PTMs) related to C-terminal Cys processing, we report herein that LanD activity can couple with radical S-adenosylmethionine chemistry to provide a new unsaturated thioether residue, S-[2-aminovinyl]-3-carbamoylcysteine, by conjugating the resultant enethiol with Cβ of the Asn residue in the C-terminal NxxC motif of a peptide for macrocyclization.

Detection of Deepfake Videos Using Long-Distance Attention.

With the rapid progress of deepfake techniques in recent years, facial video forgery can generate highly deceptive video content and bring severe security threats. And detection of such forgery videos is much more urgent and challenging. Most existing detection methods treat the problem as a vanilla binary classification problem.

Towards JPEG-Resistant Image Forgery Detection and Localization Via Self-Supervised Domain Adaptation.

With wide applications of image editing tools, forged images (splicing, copy-move, removal and etc.) have been becoming great public concerns. Although existing image forgery localization methods could achieve fairly good results on several public datasets, most of them perform poorly when the forged images are JPEG compressed as they are usually done in social networks.

Deep Learning-Based Forgery Attack on Document Images.

With the ongoing popularization of online services, the digital document images have been used in various applications. Meanwhile, there have emerged some deep learning-based text editing algorithms which alter the textual information of an image in an end-to-end fashion. In this work, we present a low-cost document forgery algorithm by the existing deep learning-based technologies to edit practical document images.

Three-Dimensional Insights into Interfacial Segregation at the Atomic Scale in a Nanocrystalline Glass-Ceramic.

The distribution of dopant atoms plays a key role in the effectiveness of doping, thereby requiring delicate characterizations. In this study, we found that energy-dispersive X-ray spectroscopy (EDX) and electron energy loss spectroscopy (EELS) techniques in scanning transmission electron microscopy (STEM) were not adequate to reveal the distribution of yttrium and the chemical composition of the ZrO/SiO heterophase interface in an yttrium-doped ZrO-SiO nanocrystalline glass-ceramic. Atom probe tomography (APT) is rarely utilized to characterize ceramics due to some inherent difficulties.

New amide alkaloids and carbazole alkaloid from the stems of Clausena lansium.

Five unreported alkaloids including four amide alkaloids (1a, 2a, 3a, and 3b) and one carbazole alkaloid (4) with two known compounds (1b, 2b) were obtained from the stems of Clausena lansium. Their structures were demonstrated by spectroscopic experiments. And the absolute configurations of compounds 1a, 1b, 2b, and 3b were determined by single X-ray diffraction analysis.

Discovery of eudesmane-type sesquiterpenoids with neuroprotective effects from the roots of Chloranthus serratus.

Seven eudesmane-type sesquiterpenoids, including three pairs of racemic compounds (1a-3a and 1b-3b) and a sesquiterpenoid lactone (4), were obtained from the roots of Chloranthus serratus. The structures of these sesquiterpenoids were characterized based on spectroscopic analyses, ECD calculations, and X-ray diffraction experiment. Neuroprotection assays of the isolated eudesmane-type sesquiterpenoids were conducted on HO damaged PC12 cells.

Robust Estimation of Upscaling Factor on Double JPEG Compressed Images.

As one of the most important topics in image forensics, resampling detection has developed rapidly in recent years. However, the robustness to JPEG compression is still challenging for most classical spectrum-based methods, since JPEG compression severely degrades the image contents and introduces block artifacts in the boundary of the compression grid. In this article, we propose a method to estimate the upscaling factors on double JPEG compressed images in the presence of image upscaling between the two compressions.

Chemical constituents of Psidium guajava leaves and their antibacterial activity.

Seven undescribed compounds, namely (+)-psiflavanone A, (-)-psiflavanone A, (+)-psiflavanone B, (-)-psiflavanone B, (+)-psiketide, (-)-psiketide, and psidinone, were isolated and chirally separated from the 95%-ethanol extract of Psidium guajava leaves. (+)/(-)-Psiflavanone A and (+)/(-)-psiflavanone B are two pairs of rare enantiomeric C-benzoylated flavanones isolated from the title plant for the first time. (+)/(-)-Psiketide are a pair of enantiomeric aromatic polyketides, and psidinone is the first example of a C phenanthrenediquinone with an angle-type 6/6/6 tricyclic skeleton.

Sesquiterpenoids with neuroprotective activities from the Chloranthaceae plant Chloranthus henryi.

A rare sesquiterpenoid possessing a 6/6 bicyclic system fused with two clustered furan units and a pair of guaiane-type sesquiterpenoids were acquired from the roots of Chloranthus henryi. Their structures with absolute configurations were characterized with spectroscopic data, ECD, and X-ray diffraction analysis. All three sesquiterpenoids showed moderate neuroprotective activities on PC12 cells damaged with hydrogen peroxide.

Secure Halftone Image Steganography Based on Feature Space and Layer Embedding.

Syndrome-trellis codes (STCs) are commonly used in image steganographic schemes, which aim at minimizing the embedding distortion, but most distortion models cannot capture the mutual interaction of embedding modifications (MIEMs). In this article, a secure halftone image steganographic scheme based on a feature space and layer embedding is proposed. First, a feature space is constructed by a characterization method that is designed based on the statistics of 4 ×4 pixel blocks in halftone images.

Novel oligomeric neolignans with PTP1B inhibitory activity from the bark of Magnolia officinalis var. biloba.

The barks of Magnolia officinalis var. biloba, Magnoliae cortex, have been used as traditional Chinese medicines for several centuries. In this study, phytochemical investigation of M.

Oxygen Defects in β-MnO Enabling High-Performance Rechargeable Aqueous Zinc/Manganese Dioxide Battery.

Rechargeable aqueous Zn/manganese dioxide (Zn/MnO) batteries are attractive energy storage technology owing to their merits of low cost, high safety, and environmental friendliness. However, the β-MnO cathode is still plagued by the sluggish ion insertion kinetics due to the relatively narrow tunneled pathway. Furthermore, the energy storage mechanism is under debate as well.

Neuroprotective racemic germacranolides from the roots of Chloranthus henryi.

Three pairs of new germacranolides, (+)/(-)-chlogermacrones A-C, along with two known analogues were obtained from the roots of Chloranthus henryi. Spectroscopic techniques and single-crystal X-ray crystallographic analyses were used for the structure elucidation of the compounds. All of the isolated compounds were tested for their neuroprotective effects on HO damaged PC12 cells, compounds 3 and 5 increased cell viability from 43.

Neuroprotective triterpene saponins from the leaves of .

Two new triterpene saponins, namely notoginsenoside Ng5 () and notoginsenoside Ng6 () were isolated from the leaves of , along with five known ones. Their structures were determined by chemical methods, NMR and X-ray experiments. The absolute configuration of compound with four sugar units was confirmed by single crystal X-ray analysis.

Cytotoxic 9,19-cycloartane Triterpenoids from the Roots of Actaea dahurica.

Eight undescribed 9,19-cycloartane type triterpenoid glycosides (cimdalglnoside A-H) and ten known analogues were obtained from the phytochemical research on the roots of Actaea dahurica (syn. Cimicifuga dahurica). All compounds were characterised by spectroscopic experiments, and chemical method.

Dihydroagarofuran sesquiterpenoids esterified with organic acids from the leaves of Tripterygium wilfordii.

Six new and one known dihydroagarofuran sesquiterpenoids esterified with organic acids were obtained from the leaves of Tripterygium wilfordii. Spectroscopic techniques (UV, IR, MS, NMR, ORD and CD) were used for the structure elucidation of the compounds. The structures of compounds 1 and 2 were confirmed by X-ray single crystallographic analyses.

Magmenthanes A-H: Eight new meroterpenoids from the bark of Magnolia officinalis var. Biloba.

Eight new meroterpenoids with different types of monoterpene units, namely, magmenthanes A-H (1-8), were identified from the bark of Magnolia officinalis var. biloba. Magmenthane A (1) possesses a 1,3-dioxabicyclo [4.

Efficient Encrypted Images Filtering and Transform Coding with Walsh-Hadamard Transform and Parallelization.

Since homomorphic encryption operations have high computational complexity, image applications based on homomorphic encryption are often time consuming, which makes them impractical. In this paper, we study efficient encrypted image applications with the encrypted domain Walsh-Hadamard transform (WHT) and parallel algorithms. We first present methods to implement real and complex WHTs in the encrypted domain.

Diterpenoids and lignans from the leaves of Tripterygium wilfordii.

Three new diterpenoids (1-3) and three new esterifying lignans (4-6) were isolated from the leaves of Tripterygium wilfordii. The structures of these compounds were elucidated on the basis of spectroscopic techniques (UV, IR, NMR, HRESIMS, and ECD method). At 10 μmol/L, compounds 4-6 showed moderate inhibitory effects on nitric oxide production in LPS-induced macrophages with inhibitory rate >80%.

Hepatoprotective glycosides from the rhizomes of Imperata cylindrical.

Three new C-methylated phenylpropanoid glycosides (1, 2), a new 8-4'-oxyneolignan (3), together with two known analogs (4, 5), were isolated from the rhizomes of Imperata cylindrical Beauv. var. major (Nees) C.

Two new saponins from the leaves of Panax notoginseng.

Two new saponins, notoginsenosides Ng1 (1) and Ng2 (2), together with seven known compounds (3-9), were isolated from the leaves of Panax notoginseng. Their structures were elucidated by UV, IR, HRESIMS, and NMR experiments. Compounds 6 and 7 showed moderate cytotoxic activities against HCT-116, with IC values of 4.

Multi-Scale Fusion for Improved Localization of Malicious Tampering in Digital Images.

A sliding window-based analysis is a prevailing mechanism for tampering localization in passive image authentication. It uses existing forensic detectors, originally designed for a full-frame analysis, to obtain the detection scores for individual image regions. One of the main problems with a window-based analysis is its impractically low localization resolution stemming from the need to use relatively large analysis windows.

Statistical model of JPEG noises and its application in quantization step estimation.

In this paper, we present a statistical analysis of JPEG noises, including the quantization noise and the rounding noise during a JPEG compression cycle. The JPEG noises in the first compression cycle have been well studied; however, so far less attention has been paid on the statistical model of JPEG noises in higher compression cycles. Our analysis reveals that the noise distributions in higher compression cycles are different from those in the first compression cycle, and they are dependent on the quantization parameters used between two successive cycles.

Blind detection of median filtering in digital images: a difference domain based approach.

Recently, the median filtering (MF) detector as a forensic tool for the recovery of images' processing history has attracted wide interest. This paper presents a novel method for the blind detection of MF in digital images. Following some strongly indicative analyses in the difference domain of images, we introduce two new feature sets that allow us to distinguish a median-filtered image from an untouched image or average-filtered one.