16S rRNA and transcriptome analysis revealed the regulatory mechanism of Romboutsia lituseburensis on serum immunoglobulin levels in geese.

Romboutsia is a dominant genus in the goose intestine. Recent studies have suggested that Romboutsia lituseburensis might regulate serum immunoglobulin levels in female geese, although the underlying mechanisms remain unclear. In this study, we administered Romboutsia lituseburensis (R.

Transcriptome analysis of testis and epididymis identifies key genes and pathways regulating gander sperm motility.

Sperm motility is a critical indicator of semen quality and determines the reproduction performance in male poultry. However, compared to chickens and ducks, very little is known about the physiological basis of varying levels of sperm motility as well the underlying regulatory mechanisms in geese. To address this, in the present study, a systematic comparison of semen quality parameters and histomorphological characteristics and genome-wide transcriptomic profiles of testes and epididymis were performed in ganders with high and low sperm motility.

Multi-omics joint analysis and identification of candidate genes for the rate of right ovarian degeneration in geese.

Avian species exhibit a notable asymmetry in their reproductive systems, with the left ovary maturing into a functional organ and the right ovary degenerating. This asymmetry is widespread across bird species, yet the molecular underpinnings of right ovarian degeneration are not fully understood. In this study, we constructed a cellular atlas of the degenerating right ovary in geese through single-cell transcriptome sequencing (scRNA-seq), revealing distinct cellular compositions and gene expression profiles.

Effects of different rearing systems on the development of uropygial gland in three goose breeds.

This study aimed to investigate and compare the age-dependent effects of net-floor mixed rearing system (MRS) and cage rearing system (CRS) on the development-related parameters of uropygial glands among three economically important goose breeds, including Sichuan White goose (SW), Gang goose (GE), and Landes goose (LD). All goslings were reared under the similar environmental conditions during the first 10 post-hatching weeks, and the goslings of each breed were then equally divided into the MRS and CRS groups. At weeks 30 and 43, eight geese from each experimental group per breed were randomly selected for morphometric and histological measurements of the uropygial glands, including Hematoxylin and Eosin (H&E) and oil red O-staining.

Molecular mechanisms underlying the testicular developmental variations among different lines of Tianfu Nonghua duck.

The reproductive and economic performance of male poultry depends on the testicular development and semen quality. However, most previous studies on male duck reproduction have focused on environmental impacts or developmental dynamics within a single breed, so the mechanisms of duck testicular development remain poorly understood. In this study, by taking a total of 240 males from 3 different lines (GF, BGF, and MC) of Tianfu Nonghua duck during the first 24 post-hatch weeks as the experimental objects, we comprehensively analyzed the age-dependent variations in the testicular histomorphological characteristics, serum testosterone (T) contents, and hypothalamic-pituitary-testicular (HPT) axis-related genes expression profiles, aiming to unravel the molecular mechanisms underlying these observed variations.

Identification of the crucial circ-mi-mRNA interaction networks regulating testicular development and spermatogenesis in ganders.

Semen quality has an important impact on the reproductive performance of ganders, and the quantity and quality of spermatozoa in semen are the determinants of semen quality. In our practical work, a small number of azoospermic ganders were observed in adult goose breeding populations, but the underlying regulatory mechanisms remain unknown. In the present study, we firstly compared the morphological and histological differences in the testes of ganders from normozoospermic group (NG) and azoospermic group (AG), and then analyzed the testicular expression patterns of circRNAs, miRNAs, and mRNAs between the two groups by using whole-transcriptome sequencing technology.

Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells.

, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of in goose follicular GCs. Gene Ontology (GO) analysis revealed that in the four groups (phGC: over_vs_over-NC; hGC: over_vs_over-NC; phGC: si_vs_si-NC; hGC: si_vs_si-NC), most differentially expressed genes (DEGs) were significantly enriched ( < 0.

Molecular mechanisms of libido influencing semen quality in geese through the hypothalamic-pituitary-testicular-external genitalia axis.

Libido plays a crucial role in influencing semen quality, yet the underlying regulatory mechanisms remain unclear. As a central axis in male goose reproduction, the hypothalamic-pituitary-testicular-external genitalia (HPTE) axis may contribute to the regulation of this process. In this study, we established a rating scale for goose libido based on average number of massages to erection (ANM) and the erection type, and evaluated semen quality across the entire flock.

Diagnosis and treatment recommendations for glucose transporter 1 deficiency syndrome.

Background: Glucose transporter 1 deficiency syndrome (Glut1DS) was initially reported by De Vivo and colleagues in 1991. This disease arises from mutations in the SLC2A1 and presents with a broad clinical spectrum. It is a treatable neuro-metabolic condition, where prompt diagnosis and initiation of ketogenic dietary therapy can markedly enhance the prognosis.

ASAP1 promotes extrahepatic cholangiocarcinoma progression by regulating the Wnt/β-catenin pathway in vitro and in vivo.

This study sought to identify the relationship between ADP-ribosylation factor GTPase-activating protein (ASAP1) expression and clinical outcomes in extrahepatic cholangiocarcinoma (EHCC) patients. Quantitative real-time PCR (qRT-PCR), Western blotting, and immunohistochemistry were used to analyze the expression of ASAP1 in cholangiocarcinoma (CC) tissue samples and cell lines. The survival rate and clinicopathological characteristics of CC patients were also examined.

Molecular mechanisms underlying age-dependent effects of rearing system on the goose testicular development and semen quality.

As an important non-genetic factor, the rearing system has significant effects on male poultry reproductive system development. However, compared with other poultry such as chickens and ducks, less is known about the effects and mechanisms of rearing system on the gander reproductive organ development and semen quality. In the present study, the testicular morphological, histological, and transcriptomic responses of three goose breeds to the two dryland rearing systems (i.

Integration of GWAS and transcriptomic analyses reveal candidate genes for duck gonadal development during puberty onset.

Background: Puberty onset signifies the beginning of sexual maturation and reproductive phase in poultry indeed, and plays an essential role in genetics and breeding. Studying gonadal development is one of the important approaches to exploring the genetic mechanism of puberty onset.

Result: In our study, the phenotype data of the testes and ovaries of the 120-day-old Nonghua duck showed a large coefficient of variation, indicating that their gonads were in different developmental states.

Combined analyses of mRNA and miRNA transcriptome reveal the molecular mechanisms of theca cells physiological differences in geese follicular selection stage.

In avian, follicular selection is a key molecular event that can determine avian egg production. Theca cells (TC) are the main components of follicles, the molecular mechanisms about TCs physiological differences during follicle selection stage are still unclear. This study revealed significant differences in proliferation, apoptosis, lipid synthesis, and steroid secretion levels between prehierarchical theca cells (phTC) and hierarchical theca cells (hTC) of Tianfu meat-type geese.

Comparative transcriptomic analysis revealed potential mechanisms regulating the hypertrophy of goose pectoral muscles.

Pectoral muscle development is an important economic trait. According to the different essence, muscle development can be divided into 2 processes: embryonic muscle fiber generation and postnatal muscle fiber hypertrophy, and postnatal muscle fiber hypertrophy has a greater impact on muscle development than the number of muscle fibers formed during the embryonic phase in poultry. However, the underlying mechanisms regulating the hypertrophy of goose pectoral muscles have not been elucidated.

Developmental variations of the reproductive organs of ganders from different goose breeds and the underlying mechanisms.

A deep understanding of the dynamics and mechanisms of male reproductive tract development is necessary for adoption of either genetic techniques or environmental management practices for improving fertility and hatchability in poultry. However, compared with other poultry such as chickens and ducks, less is known about the age- and breed-related changes in the reproductive tract development of domestic goose ganders exhibiting relatively poor reproductive performance as well as the regulatory mechanisms. In the present study, by taking 2 Chinese domestic goose breeds (Sichuan White goose, SW and Gang goose, GE; Anser cygnoides) and one European goose breed (Landes goose, LD; Anser anser) as the experimental objects, we comprehensive analyzed the morphological, histological, and genome-wide transcriptomic variations in their testicular and external genital development during the period from hatching to sexual maturity.

A Learnable and Explainable Wavelet Neural Network for EEG Artifacts Detection and Classification.

Electroencephalography (EEG) artifacts are very common in clinical diagnosis and can heavily impact diagnosis. Manual screening of artifact events is labor-intensive with little benefit. Therefore, exploring algorithms for automatic detection and classification of EEG artifacts can significantly assist clinical diagnosis.

Brown adipose tissue-derived FGF21 mediates the cardioprotection of dexmedetomidine in myocardial ischemia/reperfusion injury.

Brown adipose tissue (BAT) plays a critical role in regulating cardiovascular homeostasis through the secretion of adipokines, such as fibroblast growth factor 21 (FGF21). Dexmedetomidine (DEX) is a selective α2-adrenergic receptor agonist with a protection against myocardial ischemia/reperfusion injury (MI/RI). It remains largely unknown whether or not BAT-derived FGF21 is involved in DEX-induced cardioprotection in the context of MI/RI.

The Association Between Grandchild Care and Biological Aging Among Middle-Aged and Older Adults in China.

Background And Objectives: Substantial evidence documents grandchild care is associated with self-reported health, life satisfaction, and depressive symptoms among middle-aged and older adults. However, little is known about the relationship between grandchild care and biological aging, especially in China, which emphasizes the unique cultural value of family. The current study sheds light on the biological consequence of grandchild care by examining the link between grandchild care and biological aging among middle-aged and older adults in China, and how gender and spousal involvement in caregiving affect this link.

Transcriptome-metabolome reveals the molecular changes in meat production and quality in the hybrid populations of Sichuan white goose.

Hybrid breeding has proven to enhance meat quality and is extensively utilized in goose breeding. Nevertheless, there is a paucity of research investigating the molecular mechanisms that underlie the meat quality of hybrid geese. In this study, we employed the Sichuan White Goose as the maternal line for hybridization with the Zhedong White Goose and Tianfu Meat Goose P3 line.

Effects of rearing systems (cage versus floor) on the microbial composition and transcriptome of goose ileum.

There is a gradual transition from water to dryland rearing of geese. In this study, we performed 16S rRNA sequencing (16S rRNA-seq) and transcriptome sequencing (RNA-seq) to reveal the effects of cage rearing (CR) and floor rearing (FR) systems on the microbial composition and transcriptome of the goose ileum. Through 16S rRNA-seq, Linear Discriminant Analysis Effect Size (LEfSe) analysis identified 2 ( and ) and 14 (, , , etc.

DEPDC5 protects CD8 T cells from ferroptosis by limiting mTORC1-mediated purine catabolism.

Peripheral CD8 T cell number is tightly controlled but the precise molecular mechanism regulating this process is still not fully understood. In this study, we found that epilepsy patients with loss of function mutation of DEPDC5 had reduced peripheral CD8 T cells, and DEPDC5 expression positively correlated with tumor-infiltrating CD8 T cells as well as overall cancer patient survival, indicating that DEPDC5 may control peripheral CD8 T cell homeostasis. Significantly, mice with T cell-specific Depdc5 deletion also had reduced peripheral CD8 T cells and impaired anti-tumor immunity.

Genome-wide association analysis explores the genetic loci of amino acid content in duck's breast muscle.

Background: Amino acids are the basic components of protein and an important index to evaluate meat quality. With the rapid development of genomics, candidate regions and genes affecting amino acid content in livestock and poultry have been gradually revealed. Hence, genome-wide association study (GWAS) can be used to screen candidate loci associated with amino acid content in duck meat.