Transcriptome analysis of testis and epididymis identifies key genes and pathways regulating gander sperm motility.

Sperm motility is a critical indicator of semen quality and determines the reproduction performance in male poultry. However, compared to chickens and ducks, very little is known about the physiological basis of varying levels of sperm motility as well the underlying regulatory mechanisms in geese. To address this, in the present study, a systematic comparison of semen quality parameters and histomorphological characteristics and genome-wide transcriptomic profiles of testes and epididymis were performed in ganders with high and low sperm motility.

Multi-omics joint analysis and identification of candidate genes for the rate of right ovarian degeneration in geese.

Avian species exhibit a notable asymmetry in their reproductive systems, with the left ovary maturing into a functional organ and the right ovary degenerating. This asymmetry is widespread across bird species, yet the molecular underpinnings of right ovarian degeneration are not fully understood. In this study, we constructed a cellular atlas of the degenerating right ovary in geese through single-cell transcriptome sequencing (scRNA-seq), revealing distinct cellular compositions and gene expression profiles.

Effects of different rearing systems on the development of uropygial gland in three goose breeds.

This study aimed to investigate and compare the age-dependent effects of net-floor mixed rearing system (MRS) and cage rearing system (CRS) on the development-related parameters of uropygial glands among three economically important goose breeds, including Sichuan White goose (SW), Gang goose (GE), and Landes goose (LD). All goslings were reared under the similar environmental conditions during the first 10 post-hatching weeks, and the goslings of each breed were then equally divided into the MRS and CRS groups. At weeks 30 and 43, eight geese from each experimental group per breed were randomly selected for morphometric and histological measurements of the uropygial glands, including Hematoxylin and Eosin (H&E) and oil red O-staining.

Molecular mechanisms underlying the testicular developmental variations among different lines of Tianfu Nonghua duck.

The reproductive and economic performance of male poultry depends on the testicular development and semen quality. However, most previous studies on male duck reproduction have focused on environmental impacts or developmental dynamics within a single breed, so the mechanisms of duck testicular development remain poorly understood. In this study, by taking a total of 240 males from 3 different lines (GF, BGF, and MC) of Tianfu Nonghua duck during the first 24 post-hatch weeks as the experimental objects, we comprehensively analyzed the age-dependent variations in the testicular histomorphological characteristics, serum testosterone (T) contents, and hypothalamic-pituitary-testicular (HPT) axis-related genes expression profiles, aiming to unravel the molecular mechanisms underlying these observed variations.

Preventive effects of progressive resistance training of different intensities on breast cancer-related lymphedema.

Purpose: Explore the preventive effects of varying intensity progressive resistance exercise on breast cancer-related lymphedema.

Methods: A total of 114 breast cancer patients who underwent axillary lymph node dissection at Tangshan People's Hospital from January to April 2024 were included. Participants were randomly assigned to three groups: the control group received conventional care; intervention group 1 received conventional care + low-intensity progressive resistance exercise; and intervention group 2 received routine nursing + moderate and high-intensity progressive resistance exercise.

Identification of the crucial circ-mi-mRNA interaction networks regulating testicular development and spermatogenesis in ganders.

Semen quality has an important impact on the reproductive performance of ganders, and the quantity and quality of spermatozoa in semen are the determinants of semen quality. In our practical work, a small number of azoospermic ganders were observed in adult goose breeding populations, but the underlying regulatory mechanisms remain unknown. In the present study, we firstly compared the morphological and histological differences in the testes of ganders from normozoospermic group (NG) and azoospermic group (AG), and then analyzed the testicular expression patterns of circRNAs, miRNAs, and mRNAs between the two groups by using whole-transcriptome sequencing technology.

Transcriptome Analysis Reveals the Molecular Mechanism of PLIN1 in Goose Hierarchical and Pre-Hierarchical Follicle Granulosa Cells.

, a member of the PAT family, is expressed in both adipocytes and steroidogenic cells. In this study, we used cell transfection technology combined with transcriptome sequencing to investigate the regulatory mechanism of in goose follicular GCs. Gene Ontology (GO) analysis revealed that in the four groups (phGC: over_vs_over-NC; hGC: over_vs_over-NC; phGC: si_vs_si-NC; hGC: si_vs_si-NC), most differentially expressed genes (DEGs) were significantly enriched ( < 0.

A randomized controlled trial: Effects of compression therapy combined with exercise on chemotherapy-induced peripheral neuropathy in patients with breast cancer.

Purpose: To investigate the effects of compression therapy combined with exercise for cancer patients (EXCAP) in patients with peripheral neuropathy caused by breast cancer chemotherapy.

Methods: Overall, 108 patients with peripheral neuropathy after chemotherapy for breast cancer were randomly divided into the control group (routine nursing), experimental group 1 (compression therapy), and experimental group 2 (compression therapy and EXCAP). The National Institute of Cancer Drug Toxicity Rating Scale and the Chemotherapy-Induced Peripheral Neuropathy Assessment Tool were assessed and compared between groups.

Effects of dynamic and static relaxation therapy on cancer-induced fatigue and sleep disorders in patients with breast cancer undergoing chemotherapy: a randomized control trial.

Purpose: To investigate the effects of dynamic-static combined relaxation therapy on fatigue and sleep disorders in breast cancer patients undergoing chemotherapy.

Methods: A total of 114 patients receiving chemotherapy at Tangshan People's Hospital (September 2023-June 2024) were randomly divided into three groups: control (routine nursing), experiment group 1 (static Benson relaxation), and experiment group 2 (dynamic yoga + static Benson relaxation). The intervention lasted 8 weeks.

Effects of intermittent hand-foot hypothermia therapy on chemotherapy-induced peripheral neurotoxicity.

Purpose: Chemotherapy-induced peripheral neuropathy (CIPN) is a common side effect in patients with breast cancer undergoing chemotherapy. This study aimed to assess the effects of three different intermittent hypothermia temperatures applied to the hands and feet on CIPN symptoms in patients with breast cancer undergoing chemotherapy.

Methods: In total, 108 patients were randomly divided into three experimental groups (n = 36).

Molecular mechanisms underlying age-dependent effects of rearing system on the goose testicular development and semen quality.

As an important non-genetic factor, the rearing system has significant effects on male poultry reproductive system development. However, compared with other poultry such as chickens and ducks, less is known about the effects and mechanisms of rearing system on the gander reproductive organ development and semen quality. In the present study, the testicular morphological, histological, and transcriptomic responses of three goose breeds to the two dryland rearing systems (i.

Integration of GWAS and transcriptomic analyses reveal candidate genes for duck gonadal development during puberty onset.

Background: Puberty onset signifies the beginning of sexual maturation and reproductive phase in poultry indeed, and plays an essential role in genetics and breeding. Studying gonadal development is one of the important approaches to exploring the genetic mechanism of puberty onset.

Result: In our study, the phenotype data of the testes and ovaries of the 120-day-old Nonghua duck showed a large coefficient of variation, indicating that their gonads were in different developmental states.

Combined analyses of mRNA and miRNA transcriptome reveal the molecular mechanisms of theca cells physiological differences in geese follicular selection stage.

In avian, follicular selection is a key molecular event that can determine avian egg production. Theca cells (TC) are the main components of follicles, the molecular mechanisms about TCs physiological differences during follicle selection stage are still unclear. This study revealed significant differences in proliferation, apoptosis, lipid synthesis, and steroid secretion levels between prehierarchical theca cells (phTC) and hierarchical theca cells (hTC) of Tianfu meat-type geese.

Comparative transcriptomic analysis revealed potential mechanisms regulating the hypertrophy of goose pectoral muscles.

Pectoral muscle development is an important economic trait. According to the different essence, muscle development can be divided into 2 processes: embryonic muscle fiber generation and postnatal muscle fiber hypertrophy, and postnatal muscle fiber hypertrophy has a greater impact on muscle development than the number of muscle fibers formed during the embryonic phase in poultry. However, the underlying mechanisms regulating the hypertrophy of goose pectoral muscles have not been elucidated.

Hydrothermal nickel selenides as efficient electrodes in alkaline media: application to supercapacitors and the methanol oxidation reaction.

The advancement of active electrochemical materials is pivotal for enhancing energy conversion and storage technologies, which is essential for a sustainable future. Furthermore, achieving cost-effective technologies necessitates avoiding the use of noble metals and low-throughput processes that require high vacuum or high temperatures. Herein, we describe in detail a simple solution-based protocol to obtain a series of phase-controlled nickel selenide nanomaterials.

Developmental variations of the reproductive organs of ganders from different goose breeds and the underlying mechanisms.

A deep understanding of the dynamics and mechanisms of male reproductive tract development is necessary for adoption of either genetic techniques or environmental management practices for improving fertility and hatchability in poultry. However, compared with other poultry such as chickens and ducks, less is known about the age- and breed-related changes in the reproductive tract development of domestic goose ganders exhibiting relatively poor reproductive performance as well as the regulatory mechanisms. In the present study, by taking 2 Chinese domestic goose breeds (Sichuan White goose, SW and Gang goose, GE; Anser cygnoides) and one European goose breed (Landes goose, LD; Anser anser) as the experimental objects, we comprehensive analyzed the morphological, histological, and genome-wide transcriptomic variations in their testicular and external genital development during the period from hatching to sexual maturity.

Volume replacement in tumor plastic surgery and breast-conserving surgery using 3D grid and strip‑shaped acellular dermal matrix: Two case reports.

The present study was driven by the scarcity of suitable materials for mending partial breast defects and the imperative considerations of safety and durability. The current study presents findings from two female patients, aged 59 and 40, who underwent breast cancer treatment. Patient 1 underwent a mastectomy with a sentinel lymph node biopsy, while patient 2 underwent a partial mastectomy with axillary lymph node dissection.

Transcriptome-metabolome reveals the molecular changes in meat production and quality in the hybrid populations of Sichuan white goose.

Hybrid breeding has proven to enhance meat quality and is extensively utilized in goose breeding. Nevertheless, there is a paucity of research investigating the molecular mechanisms that underlie the meat quality of hybrid geese. In this study, we employed the Sichuan White Goose as the maternal line for hybridization with the Zhedong White Goose and Tianfu Meat Goose P3 line.

Effects of rearing systems (cage versus floor) on the microbial composition and transcriptome of goose ileum.

There is a gradual transition from water to dryland rearing of geese. In this study, we performed 16S rRNA sequencing (16S rRNA-seq) and transcriptome sequencing (RNA-seq) to reveal the effects of cage rearing (CR) and floor rearing (FR) systems on the microbial composition and transcriptome of the goose ileum. Through 16S rRNA-seq, Linear Discriminant Analysis Effect Size (LEfSe) analysis identified 2 ( and ) and 14 (, , , etc.

Genome-wide association study reveals the candidate genes of humerus quality in laying duck.

Bone plays a crucial role in poultry's health and production. However, during the selection and cage farming, there has been a decline in bone quality. As the development of breeding theory, researchers find that it's possible to enhance bone quality through selective breeding.

Genome-wide association analysis explores the genetic loci of amino acid content in duck's breast muscle.

Background: Amino acids are the basic components of protein and an important index to evaluate meat quality. With the rapid development of genomics, candidate regions and genes affecting amino acid content in livestock and poultry have been gradually revealed. Hence, genome-wide association study (GWAS) can be used to screen candidate loci associated with amino acid content in duck meat.

Exploring the dynamic three-dimensional chromatin architecture and transcriptional landscape in goose liver tissues underlying metabolic adaptations induced by a high-fat diet.

Background: Goose, descendants of migratory ancestors, have undergone extensive selective breeding, resulting in their remarkable ability to accumulate fat in the liver and exhibit a high tolerance for significant energy intake. As a result, goose offers an excellent model for studying obesity, metabolic disorders, and liver diseases in mammals. Although the impact of the three-dimensional arrangement of chromatin within the cell nucleus on gene expression and transcriptional regulation is widely acknowledged, the precise functions of chromatin architecture reorganization during fat deposition in goose liver tissues still need to be fully comprehended.

Impacts of longitudinal water curtain cooling system on transcriptome-related immunity in ducks.

Background: The closed poultry houses integrated with a longitudinal water curtain cooling system (LWCCS) are widely used in modern poultry production. This study showed the variations in environmental conditions in closed houses integrated with a longitudinal water curtain cooling system. We evaluated the influence of different environmental conditions on duck growth performance and the transcriptome changes of immune organs, including the bursa of Fabricius and the spleen.

Genome-wide association study for bone quality of ducks during the laying period.

The cage-rearing model of the modern poultry industry makes the bones of birds, especially egg-laying birds, more vulnerable to fracture, which poses serious damage to the health of birds. Research confirms that genetic material plays an important role in regulating bone growth, development, and remodeling. However, the genetic architecture underlying bone traits is not well understood.