miR-Cabiner: A Universal microRNA Sensing Platform Based on Self-Stacking Cascaded Bicyclic DNA Circuit-Mediated CRISPR/Cas12a.

CRISPR/Cas12a-based diagnostics have great potential for sensing nucleic acids, but their application is limited by the sequence-dependent property. A platform termed miR-Cabiner (a universal NA sensing platform based on self-stacking scaded cyclic DA circuit-mdiated CISPR/Cas12a) is demonstrated herein that is sensitive and universal for analyzing miRNAs. This platform combines catalytic hairpin assembly (CHA) and hybrid chain reaction (HCR) into a unified circuit and finally cascades to CRISPR/Cas12a.

Host-Guest Structure Enabling Electrocatalytic Hydrogen Evolution Performance by POM@TM-BDC Composites.

Developing economical, efficient, and earth-rich electrocatalysts for hydrogen evolution reaction (HER) is quite challenging and ideal. We propose that [PWO] as the guest, due to its excellent reversible 18 electron-transfer capacity and redox properties, and then TM-BDC (TM = Ni, Co, Fe, BDC = 1,4-benzene-dicarboxylate) as the host make [PWO] packaged and not escape due to its porous structure. Benefiting from strong redox-competent interactions between [PWO] and porous structures of TM-BDC and full exposure of abundant active sites, three {PW}@TM-BDC composites exhibited excellent HER activity, with {PW}@Ni-BDC requiring 198 mV (overpotentials) and 104 mV/dec (Tafel slope) for HER.

Individualized Physical Activity Prescriptions for Children and Adolescents With Cancer: A Systematic Review and Meta-analysis of Randomized Controlled Trials.

Background: For improved health outcomes, children and adolescents with cancer must maintain physical activity. Individualized physical activity prescription is an effective way to promote physical activity in this group.

Objective: We reviewed and meta-analyzed evidence on the effectiveness of individualized physical activity prescriptions for improving physical activity and other health outcomes among children and adolescents with cancer.

Revealing Single-Molecule Photocurrent Generation Mechanisms under On- and Off-Resonance Excitation.

We investigate photocurrent generation mechanisms in a pentacene single-molecule junction using subnanometer resolved photocurrent imaging under both on- and off-resonance laser excitation. By employing a wavelength-tunable laser combined with a lock-in technique, net photocurrent signals are extracted to elucidate photoinduced electron tunneling processes. Under off-resonance excitation, photocurrents are found to arise from photon-assisted tunneling, with contributions from three distinct frontier molecular orbitals at different bias voltages.

Tunable Topological Transitions Probed by the Quantum Hall Effect in Twisted Double Bilayer Graphene.

The moiré system provides a tunable platform for investigating exotic quantum phases. Particularly, the displacement field is crucial for tuning the electronic structures and topological properties of twisted double bilayer graphene (TDBG). Here, we present a series of -tunable topological transitions by the evolution of quantum Hall phases (QHPs) in the valence bands of TDBG.

Effects of remote support courses on parental mental health and child development in autism: A randomized controlled trial.

Background: Sustaining the mental health of autistic children's parents can be demanding.

Aim: To determine the effect of remote support courses on the mental health of parents and the development of autistic children.

Methods: Parents of 140 autistic children were randomly assigned to two groups receiving a 2-week intervention: The control group received caregiver-mediated intervention (CMI); the experimental group received CMI with remote family psychological support courses (R-FPSC).

Genetic Ablation of p16 Mitigates Premature Osteoporosis Induced by PTHrP Nuclear Localization Sequence and C-terminal Deletion through Inhibition of Cellular Senescence.

Premature osteoporosis due to parathyroid hormone-related peptide (PTHrP) dysfunction presents significant bone health challenges. This study explores the role of p16-mediated cellular senescence in this condition using a Pthrp knock-in (KI) mouse model lacking the nuclear localization sequence and C-terminus of PTHrP. We generated p16⁻⁄⁻KI mice and compared them with wild-type, p16⁻⁄⁻, and KI mice.

RUNX2 Phase Separation Mediates Long-Range Regulation Between Osteoporosis-Susceptibility Variant and XCR1 to Promote Osteoblast Differentiation.

GWASs have identified many loci associated with osteoporosis, but the underlying genetic regulatory mechanisms and the potential drug target need to be explored. Here, a new regulatory mechanism is found that a GWAS intergenic SNP (rs4683184) functions as an enhancer to influence the binding affinity of transcription factor RUNX2, whose phase separation can mediate the long-range chromatin interaction between enhancer and target gene XCR1 (a member of the GPCR family), leading to changes of XCR1 expression and osteoblast differentiation. Bone-targeting AAV of Xcr1 can improve bone formation in osteoporosis mice, suggesting that XCR1 can be a new susceptibility gene for osteoporosis.

EZH2/miR-142-3p/HMGB1 axis mediates chondrocyte pyroptosis by regulating endoplasmic reticulum stress in knee osteoarthritis.

Background: Knee osteoarthritis (OA) is still challenging to prevent or treat. Enhanced endoplasmic reticulum (ER) stress and increased pyroptosis in chondrocytes may be responsible for cartilage degeneration. This study aims to investigate the effect of ER stress on chondrocyte pyroptosis and the upstream regulatory mechanisms, which have rarely been reported.

Experimental investigation on modes of spray formation, droplet size and size distribution in a spinning disc atomizer.

The spinning disc atomizer is extensively utilized in agricultural spraying, with optimized operating conditions significantly enhancing atomization performance. In this paper, the atomization characteristics of a spinning disc were studied using photographs taken by a high-speed camera. Ethanol-water solutions were used at various flow rates and the disc speed was varied in a wide range.

Developing a universal multi-epitope protein vaccine candidate for enhanced borna virus pandemic preparedness.

Introduction: Borna disease virus 1 (BoDV-1) is an emerging zoonotic RNA virus that can cause severe acute encephalitis with high mortality. Currently, there are no effective countermeasures, and the potential risk of a future outbreak requires urgent attention. To address this challenge, the complete genome sequence of BoDV-1 was utilized, and immunoinformatics was applied to identify antigenic peptides suitable for vaccine development.

Unique clones secrete populations of extracellular vesicles with unique protein profile and variable infectious capability.

The study of extracellular vesicles has become an incredibly important field of study, but the inherent heterogeneity of these vesicles continues to make their study challenging. The genetic variability and well-documented protocols for the growth and vesicle isolation from parasites provide a unique opportunity to compare the heterogeneity of different populations secreted by clones. was cultured on solid SDM agar plates and 8 clonal colonies were selected.

High-Performing Direct X-Ray Detection Made of One-Dimensional Perovskite-Like (TMHD)SbBr Single Crystal With Anisotropic Response.

3D and 2D organic hybrid perovskites, as well as double perovskites, have demonstrated their suitability for direct X-ray detection. However, the sensitivity and stability of 3D perovskite X-ray detectors are currently being hindered by the existing constraints on ion movement. Therefore, there is an immediate need to develop X-ray detectors that are both highly sensitive and stable while also being environmentally friendly.

Functional Expression and Construction of a Self-Sufficient Cytochrome P450 Chimera for Efficient Steroidal C14α Hydroxylation in Escherichia coli.

C14-functionalized steroids enabled diverse biological activities in anti-gonadotropin and anticancer therapy. However, access to C14-functionalized steroids was impeded by the deficiency of chemical synthetic methods. Recently, several membrane-bound fungal cytochrome P450s (CYPs) have been identified with steroid C14α-hydroxylation activity.

Methylation-modulated PFTK1 regulates gefitinib resistance via Wnt/β-catenin signaling in EGFR mutant non-small-cell lung cancer cells.

Inevitable gefitinib resistance is the biggest bottleneck in current treatment and the mechanisms are not fully understood. Here, we observe that PFTK1 (also named CDK14) is significantly enhanced in NSCLC with gefitinib resistance. And the upregulation of PFTK1 is negatively associated with progression-free survival (PFS) in NSCLC patients who receive gefitinib treatment.

Development, validation, and clinical application of LC-MS/MS method for simultaneous determination of ibrutinib, zanubrutinib, orelabrutinib, acalabrutinib, and their active metabolites in patients with B-cell lymphoma.

Bruton's tyrosine kinase inhibitors (BTKis) exhibit significant interindividual pharmacokinetics, making therapeutic drug monitoring (TDM) a promising approach for personalized therapy. However, simultaneous quantification of multiple BTKis poses technical challenges. A unified protocol for BTKis detection would be clinically desirable.

Effect of Baduanjin exercise on health and functional status in patients with chronic obstructive pulmonary disease: a community-based, cluster-randomized controlled trial.

Baduanjin has been shown to be an effective method of exercise for promoting body function in patients with chronic obstructive pulmonary disease (COPD). However, this method is rarely used by rural doctors in rural communities. The aim of this study was to compare the therapeutic effects of Baduanjin exercise with those of conventional pulmonary rehabilitation (CPR) implemented by rural doctors in patients with COPD.

The accuracy of fluorine 18-labelled prostate-specific membrane antigen PET/CT and MRI for diagnosis of prostate cancer in PSA grey zone.

Background: The diagnostic utility of prostate biopsy is limited for prostate cancer (PCa) in the prostate-specific antigen (PSA) grey zone. This study aims to evaluate the diagnostic performance of multiparametric magnetic resonance imaging (mpMRI) and prostate-specific membrane antigen positron emission tomography/computed tomography (PSMA PET/CT) for PSA grey zone PCa and clinically significant PCa (csPCa).

Methods: A total of 82 patients with PSA levels ranging from 4 to 10 ng/mL who underwent F-PSMA-1007 PET/CT, mpMRI, and prostate biopsy were prospectively enrolled.

Deptor protects against myocardial ischemia-reperfusion injury by regulating the mTOR signaling and autophagy.

Deptor knockout mice were constructed by crossing Deptor Floxp3 mice with myh6 Cre mice, establishing a myocardial ischemia-reperfusion (I/R) model. Deptor knockout mice exhibited significantly increased myocardial infarction size and increased myocardial apoptosis in vivo. ELISA analysis indicated that the expression of CK-MB, LDH, and CtnT/I was significantly higher in the Deptor knockout mice.

Single-cell atlas comparison across vertebrates reveals auditory cell evolution and mechanisms for hair cell regeneration.

Mammals suffer permanent hearing impairment from the loss of auditory hair cells due to their inability to regenerate. In contrast, lower vertebrates exhibit extraordinary capacity for hair cell regeneration and hearing restoration, but the mechanisms remain unclear. Here we characterize the single-cell atlas of Xenopus laevis inner ear and perform a comprehensive comparison with mouse model.

Enhancing knee osteoarthritis diagnosis with DMS: a novel dense multi-scale convolutional neural network approach.

Background: Osteoarthritis (OA) of the knee is a prevalent chronic degenerative joint condition that is having a growing impact on a global scale., posing a challenge in diagnosis which is often reliant on time-consuming and error-prone visual analysis by physicians. There is a critical need for an automated, efficient, and accurate diagnostic method to improve early detection and treatment.

Circulating plasma derived exosomes from systemic lupus erythematosus aggravate lupus nephritis through miR-122-5p/FOXO3-mediated macrophage activation.

Systemic lupus erythematosus (SLE) is a chronic and systemic autoimmune disease characterized by dysregulation in both innate and adaptive immunity. Polarization of macrophages into M1/M2 macrophages affects the development of lupus. Exosomes-miRNA plays a crucial role in disease progression.

The lived experience of living liver donors: A meta-synthesis of qualitative studies.

Background: Living liver donors are frequently used for liver transplantation, but they experience many physical changes and psychological challenges. A better understanding of the experiences of liver donors can provide them with comprehensive and individualized patient-centred support and health care. Thus, this study sought to identify and synthesize existing qualitative studies to develop an in-depth understanding of the lived experiences of living liver donors.

Association between composite dietary antioxidant index and metabolic dysfunction-associated fatty liver disease: a cross-sectional study from NHANES.

Background: Metabolic dysfunction-associated fatty liver disease (MAFLD) is a typical hepatic steatosis with metabolic dysfunction. The composite dietary antioxidant index (CDAI) measures individual antioxidant capacity, and the relationship with MAFLD has received little attention. Our goal is to explore the association of CDAI with MAFLD.