Fabrication of norepinephrine and ε-polylysine engineered magnetic nanocomposites tailored for phosphopeptides analysis.

Protein phosphorylation is a highly prevalent post-translational modification that holds a vital position in numerous physiological processes. Prior to mass spectrometry detection, the enrichment of phosphopeptides is critically significant due to their susceptibility to interference from abundant non-phosphopeptides. In this study, the magnetic nanocomposite (FeO@NE@PL) was successfully synthesized and characterized.

pH-sensitive metal-organic framework carrier decorated with chitosan for controlled drug release.

Satisfactory chemotherapeutic efficiency depends on improving the insufficient selectivity of chemotherapeutic drugs and decreasing damages to healthy tissues/cells, hence, the reasonable design and construction of drug carriers are of great significance in tumor treatment. Herein, the pH-sensitive MOF carrier was developed via the decoration of 4-formylphenylboronic acid (4-FPBA) for controlled 5'-deoxy-5-fluorocytidine (DFCR) release with selectivity. Besides, chitosan (CS) was introduced as the "gatekeeper" for the prevention of drug leakage.

Design of pH-responsive molecularly imprinted polymer as a carrier for controlled and sustainable capecitabine release.

A molecularly imprinting polymer (MIP) carrier with pH-responsivity was designed to construct a drug delivery system (DDS) focusing on controlled and sustainable capecitabine (CAPE) release. The pH-responsive characteristic was achieved by the functionalization of SiO substrate with 4-formylphenylboronic acid, accompanied by the introduction of fluorescein isothiocyanate for the visualization of the intracellular localization of the nanocarrier. Experimental results indicated that CAPE was adsorbed onto the drug carrier with satisfactory encapsulation efficiency.

Preparation of dual-functional epitope imprinted polymers for the enrichment of transferrin.

Transferrin (TRF), a glycoprotein involved in cellular iron uptake, is a potential target for the diagnosis and treatment of several diseases and cancers. Therefore, the identification and isolation of TRF is clinically important. In this work, we prepared magnetic molecularly imprinted polymers (EMIP) based on metal chelation using norepinephrine and 3-aminophenylboronic acid as functional monomers.

Preparation of poly(caffeic acid)-coated epitope molecularly imprinted polymers and investigation of adsorption performance toward ovalbumin.

Food allergy can lead to severe allergic reactions that are potentially fatal for human, hence the detection of food allergens such as ovalbumin (OVA) is important. In this study, a poly(caffeic acid)-coated epitope molecularly imprinted polymer (EMIP) was prepared by chelation and autoxidation of caffeic acid with hexamethylenediamine. EMIP has not only imprinted cavities highly matched with OVA in size and spatial structure, but also externally abundant hydrophilic groups, resulting in few non-specific binding and good hydrophilicity.

Dual-ligand hydrogen-bonded organic framework: Tailored for mono-phosphopeptides and glycopeptides analysis.

Hydrogen-bonded organic frameworks (HOFs) have emerged as a promising class of materials for applications of separation and enrichment. Utilizing multiple-ligands to construct HOFs is a promising avenue towards the development of structurally stable and functionally diverse frameworks, offering opportunities to create customized binding sites for selective recognition of biomolecules. In recent years, due to the crucial role that protein post-translational modifications (PTMs) play in maintaining protein function and regulating signaling pathways, and the growing recognition of the extensive cross-talk that can occur between PTMs, simultaneous analysis of different types of PTMs represents a requirement of a new generation of enrichment materials.

Design of reversibly charge-changeable rhodamine B modified magnetic nanoparticles to enrich phosphopeptides.

In the present study, by employing ethylenediaminetetraacetic acid (EDTA), tetraethylene pentaamine (TEPA), and rhodamine B (Rb), we designed and synthesized a magnetic adsorbent (FeO@EDTA@TEPA@Rb) on the basis of reversible charge change of Rb and applied to capture phosphopeptides. Rb existing in open planarized zwitterion form when stimulated by acidic loading buffer adsorbs negative phosphopeptides via electrostatic interaction. Under the stimulation of alkalic eluent, ring-closed structure of Rb is formed to elute the enriched phosphopeptides.

Design of a hydrophilic mercaptosuccinic acid-functionalized β-cyclodextrin polymer host-guest interaction: toward highly efficient glycopeptide enrichment.

A hydrophilic interaction chromatography (HILIC) strategy is considered as an efficient strategy for efficient glycopeptide enrichment. Here, a novel hydrophilic material (denoted as magCDP@Ada-MSA) was constructed through host-guest interaction between crosslinked β-cyclodextrin (β-CD) polymers and mercaptosuccinic acid (MSA) derived adamantane. On the one hand, crosslinked β-CD polymers have great hydrophilicity due to their abundant hydrophilic hydroxyl groups.

Tailoring a multifunctional magnetic cationic metal-organic framework composite for synchronous enrichment of phosphopeptides/glycopeptides.

Herein, for the first time, a multifunctional magnetic cationic MOF composite (FeO@ILI-01@Ti) was successfully prepared for the synchronous enrichment of phosphopeptides/glycopeptides. The as-prepared FeO@ILI-01@Ti bears attractive properties like abundant surface positive charge and excellent hydrophilicity, ensuring that phosphopeptides/glycopeptides can be well captured based on electrostatic attraction and hydrophilic interaction. By integrating FeO@ILI-01@Ti and mass spectrometry (MS) determination, high sensitivity (0.

Glutathione-functionalized magnetic thioether-COFs for the simultaneous capture of urinary exosomes and enrichment of exosomal glycosylated and phosphorylated peptides.

Exosomes play an irreplaceable role in physiological and pathological processes, and the study of proteomics (especially protein post-translational modifications, PTMs) in exosomes can reveal the pathogenesis of diseases and screen therapeutic disease targets. The separation and enrichment process is an essential step in mass spectroscopy-based exosomal PTMs studies to reduce sample complexity and ionization-suppression effects. Herein, we designed a novel magnetic zwitterionic material, namely glutathione-functionalized thioether covalent organic frameworks (FeO@Thio-COF@Au@GSH), possessing fast magnetic responsiveness, regular porosity, and a suitable surface area.

Design of a Spiropyran-Based Smart Adsorbent with Dual Response: Focusing on Highly Efficient Enrichment of Phosphopeptides.

Smart responsive materials have attractive application prospects due to their tunable behaviors. In this work, we design novel spiropyran (SP)-based magnetic nanoparticles (MNP-SP) with dual response to ultraviolet light and pH and apply them to the enrichment of phosphopeptides. SP is modified on the surface of magnetic nanoparticles through a simple esterification reaction, based on which an MNP-SP-MS phosphopeptide identification platform is established.

[Recent developments of pesticide adsorbents based on cyclodextrins].

The invention and application of pesticides have greatly increased the yield of crops, greatly contributing to ensuring people's basic livelihoods and gradually improving their livelihoods to a well-off level. However, foods, water sources, and soil, containing high levels of pesticide residues, result in increasingly serious pollution. Pesticide residues usually have the characteristics of micro toxicity, difficult biodegradation, and bioaccumulation, and thus pose serious threat to living organisms and ecosystems.

Engineering Magnetic Guanidyl-Functionalized Supramolecular Organic Framework for Efficient Enrichment of Global Phosphopeptides.

Comprehensive mass spectrometry-based proteomics analysis is currently available but remains challenging, especially for post-translational modifications of phosphorylated proteins. Herein, multifunctional magnetic pillar[5]arene supramolecular organic frameworks were fabricated and immobilized with arginine (mP5SOF-Arg) for highly effective enrichment of global phosphopeptides. The specific phosphate-P5/phosphate-guanidine affinities and large surface area with regular porosity contribute to the high enrichment capacity.

A turn-on fluorescence sensor for creatinine based on the quinoline-modified metal organic frameworks.

Creatinine (Cre) level is closely related to renal function of human. Therefore, it is of great significance to develop highly sensitive and selective tools for Cre determination. Herein, a turn-on fluorescence metal organic framework (MOF) sensor, which was synthesized by post-synthetic modification of 8-hydroxy-2-quinolinecarboxaldehyde (HQCA) and Al toward UiO-66-NH, was applied to detect Cre.

Supramolecular adsorbents in extraction and separation techniques - A review.

Sample preparation procedure before the detection step is of great importance for successful realization of an analytical method. The extraction and preconcentration efficiency, sample throughput, and application potential of a sample preparation approach are greatly dependent on adsorbents. This review (with 172 references) reveals a critical view on the latest achievements of supramolecular materials in the field of adsorption.

[Preparation of polyoxometalate-chitosan magnetic composite for the enrichment of phosphopeptides].

A method for the enrichment of phosphopeptides based on magnetic nanoparticles coated with polyoxometalate (POM) was developed. A layer-by-layer assembly technique was used to prepare magnetic nanoparticles (MNPs) coated with polyoxometalate and chitosan. The composites were used for phosphopeptide enrichment prior to analysis by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).

A modulation approach for covalent organic frameworks: Application to solid phase microextraction of phthalate esters.

A modulation approach with 4-hydroxybenzhydrazide as the modulating agent was designed for constructing 2,4,6-triphenoxy-1,3,5-benzene-based covalent organic frameworks (COFs). The COFs materials were employed as solid phase microextraction (SPME) coatings for the extraction of phthalate esters (PAEs). Various experimental conditions including extraction temperature, extraction time, salt concentration, and desorption time were investigated and optimized.

A triazine based organic framework with micropores and mesopores for use in headspace solid phase microextraction of phthalate esters.

A dual-pore covalent organic framework (COF) that contains micropores and mesopores was prepared from 2,4,6-triphenoxy-1,3,5-triazine (TPT). A building block is used in which double linking sites were introduced at each branch of a C-symmetric skeleton. The COF is shown to be a viable coating for fibers for solid-phase microextraction of phthalic acid esters (PAEs).

Development of Gd-immobilized glutathione-coated magnetic nanoparticles for highly selective enrichment of phosphopeptides.

In this study, we designed a gadolinium-based immobilized metal ion affinity chromatography material for the selective enrichment of phosphopeptides. Gadolinium ion was immobilized on the surface of glutathione-coated magnetic nanoparticles through a facile and effective synthetic route. The adsorbent integrated the advantages of superparamagnetism of FeO core, good biological compatibility of glutathione, and strong interaction between gadolinium ion and phosphopeptides.

Peanut agglutinin and β-cyclodextrin functionalized polymer monolith: Microextraction of IgG galactosylation coupled with online MS detection.

A facile online method coupling polymer monolithic microextraction (PMME) with mass spectrometry (MS) was developed for the detection of Immunoglobulin G (IgG) galactosylation glycopeptides. A peanut agglutinin-β-cyclodextrin (PNA-β-CD) functionalized poly(hydroxyethyl methylacrylate-ethyleneglycol dimethacrylate) monolith was designed via a click reaction. Thanking to the specificity of PNA-β-CD for the targets, the material exhibited enhanced enrichment selectivity and extraction efficiency for IgG galactosylation glycopeptides.

Specific enrichment of glycoproteins with polymer monolith functionalized with glycocluster grafted β-cyclodextrin.

The low abundance of glycoproteins in complex samples results in the prerequisite role of efficient and selective enrichment of them. In the present work, we designed a new kind of glycosylation poly(hydroxyethyl methacrylate-pentaerythritol triacrylate) monolith functionalized with glycocluster grafted β-cyclodextrin for the enrichment of glycoproteins. The introduced modifiers endowed the monolithic material with enhanced hydrophilicity and surface area, which benefitted to improve the enrichment selectivity and extraction efficiency for glycopeptides.

Highly selective enrichment of phosphopeptides by on-chip indium oxide functionalized magnetic nanoparticles coupled with MALDI-TOF MS.

Selective and efficient preconcentration is indispensable for low concentration of phosphopeptides in phosphorylated protein-related samples prior to MS-based analysis. Herein, an on-chip system coupled magnetic SPE with MALDI-TOF MS was designed. A metal oxide affinity chromatography material, indium oxide, was coated on the surface of Fe O magnetic nanoparticles to prepare the adsorbent, spatially confined with an applied magnetic field.

Design of a ruthenium(III) immobilized affinity material based on a β-cyclodextrin-functionalized poly(glycidyl methacrylate-ethylene dimethacrylate) monolith for the enrichment of hippuric acid.

Immobilized metal affinity chromatography has drawn great attention as a widespread separation and purification approach. In this work, ruthenium was firstly introduced into the preparation of immobilized metal affinity chromatography considering its affinity to N,O-donor ligands. A β-cyclodextrin-functionalized poly(glycidyl methacrylate-ethylene dimethacrylate) monolith was designed and employed as the supporting material in immobilized metal affinity chromatography.

Preparation of a polymer monolith modified with delaminated layered double hydroxides for the microextraction of β-agonists.

In the present study, a novel poly(acrylamide-co-N-isopropylacrylamide-co-ethylene dimethacrylate) monolithic column modified with saline-modified delaminated layered double hydroxides was developed and exploited as the stationary phase in the polymer monolith microextraction of β-agonists. Transmission electron microscopy, Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and thermal gravimetric analysis with derivative thermogravimetric determination were employed to characterize the monoliths. By coupling with high-performance liquid chromatography determinations, extraction parameters affecting the extraction efficiency, including sample volume, flow rate, sample pH, eluent volume, and eluent flow rate were investigated with an orthogonal experiment design, L (4 ).

Fabrication of cross-linked hydrazone covalent organic frameworks by click chemistry and application to solid phase microextraction.

Covalent organic frameworks (COFs) are an emerging class of porous organic frameworks with diverse promising applications. Herein, we presented the first example of cross-linked hydrazone COFs (cross-linked COFs) coating via thiol-ene click chemistry for solid phase microextraction (SPME). Strong covalent bonds and interlayer of the prepared networks ensured the adsorption capacity and durability of the novel SPME fiber.