[Determination of 3-hydroxyisovaleryl carnitine in plasma and urinary excretion by ultra-performance liquid chromatography-tandem mass spectrometry].

Objective: An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ) method was developed for the determination of 3-hydroxyisovaleryl carnitine in plasma and urinary excretion.

Methods: Plasma and urinary excretion samples were centrifugated with high speed at 3000 x g for 10 minutes, and the MCX solid phase extraction column was used to filter and purify the liquid samples. The following procedures were elution, wash-out, redissolve, detection.