Publications by authors named "Jiuhai Wang"

Article Synopsis
  • The study introduces an efficient method for turning human induced pluripotent stem cells (iPSCs) into functional endothelial cells (iECs) using a doxycycline-inducible system to express the ETV2 transcription factor, achieving over 90% efficiency in just four days.
  • This new method is designed to overcome challenges found in traditional transfection techniques, making it simpler and more consistent across different stem cell lines while allowing the iECs to maintain their ability to form blood vessels both in lab settings and in living organisms.
  • The generated iECs show strong similarities to primary endothelial cells in terms of gene expression and protein profiles, validating their functionality and potential for use in research and
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Amyloid aggregation is associated with many neurodegenerative diseases such as Alzheimer's disease (AD). The current technologies using phototherapy for amyloid inhibition are usually photodynamic approaches based on evidence that reactive oxygen species can inhibit Aβ aggregation. Herein, we report a novel combinational photothermally assisted photo-oxygenation treatment based on a nano-platform of the brain-targeting peptide RVG conjugated with the 2D porphyrinic PCN-222 metal-organic framework and indocyanine green (PCN-222@ICG@RVG) with enhanced photo-inhibition in Alzheimer's Aβ aggregation.

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Herein, a dual-modal fluorescent/colorimetric "Signal-On" nanoprobe based on PCN-222 nanorods (NRs) toward phosphate was proposed for the first time. Due to the high affinity of the zirconium node in PCN-222 NRs for phosphate, the structure collapse of PCN-222 NRs was triggered by phosphate, resulting in the release of the tetrakis(4-carboxyphenyl)porphyrin (TCPP) ligand from PCN-222 NRs as well as the enhancement of fluorescence and absorbance signals. The PCN-222 NR-based nanoprobe could be employed for phosphate detection over a wide concentration range with a detection limit down to 23 nM.

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Detecting trace amounts of copper ions (Cu2+) is of high importance since copper is an essential element in the environment and the human body. Despite the recent advances in Cu2+ detection, the current approaches still suffer from insensitivity and lack of in situ detection in living cells. In the present work, a fluorescent nanosensor based on porphyrinic metal-organic framework nanoparticles (MOF-525 NPs) is proposed for sensitive and selective monitoring of Cu2+ in aqueous solution and living cells.

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The aberrant aggregation of amyloid-β peptide (Aβ) in the brain has been considered as the major pathological hallmark of Alzheimer's diseases (AD). Inhibition of Aβ aggregation is considered as an attractive therapeutic intervention for alleviating amyloid-associated neurotoxicity. Here, we report the near-infrared light (NIR)-induced suppression of Aβ aggregation and reduction of Aβ-induced cytotoxicity via porphyrinic metal-organic framework (MOF) PCN-224 nanoparticles.

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The isolation of nucleic acids (NA) is the preliminary step to carry out genetic studies and DNA biosensor development. The presence of inhibitors in the purified NA interferes with the downstream application. These salts and other organic contaminations particularly challenge the analytical sensitivity of DNA biosensors.

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Nucleic acid (NA) extraction from cancer cells is an essential step in molecular oncologic testing. The conventional NA extraction protocols, based on several ultracentrifugation steps, suffer from time-consuming and complex manipulation. Here, a magnetic nanoparticle (MNP) based method for simultaneous extraction of DNA and RNA from cancer cells is described.

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Nucleic acid testing (NAT) based methods are more sensitive and specific, and are preferred over enzyme immunoassays. Different NAT based protocols have been designed to detect multiple pathogens in order to reduce the inherent high cost for detection. However, these assays do not reliably detect a large number of pathogens at once.

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RNA viruses, particularly, the highly pathogenic avian influenza (HPAI) virus, pose serious health concerns, and cause huge economic losses worldwide. Diagnostic tools for the early detection of these deadly RNA viruses are urgently needed to implement treatment and disease control strategies. Conventional reverse transcription polymerase chain reaction (RT-PCR)-based chemiluminescent (RT-PCR-CL) detection is frequently used for the diagnosis of viral infections.

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With the help of Fe3O4 nagnetic nanoparticles as a solid carrier and an excellent tool for separation, six SNP loci (rs2279115 of BCL2 gene, rs804270 of NEIL2 gene, rs909253 of LTA gene, rs2294008 of PSCA gene, rs3765524 and rs10509670 of PLCE1 gene) were selected to evaluate their relation to gastric cancer risk. Using two kinds of functionalized magnetic nanoparticles and universal tagged arrays, the whole operation procedure including genome DNA extraction and SNP genotyping was performed. All genotypes and allele frequencies were calculated in the cases and controls respectively to analyze their association with gastric cancer risk.

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