Transcriptomics and iTRAQ-proteomics analyses provide novel insights into the defense mechanism of black shank disease in tobacco.

Black shank disease caused by is one of the most important diseases in tobacco worldwide and can result in a devastating loss in tobacco cultivation. Many efforts have been carried out to identify the chromosome segment from containing a resistance locus carrying a gene named ; however, the gene has not been cloned, and knowledge of the potential mechanism of the gene in the resistant lines is limited. To further characterize the resistance mechanism of the gene, we first used the resistant line "RBST" and the susceptible cultivar "Honghuadajinyuan" (HD) to obtain the near-isogenic line RBS89 containing the gene from RBST.