Publications by authors named "Jitka Danadova"
Article Synopsis
- - Cyclin A1 and Cyclin A2 are two members of the cyclin A family found in various species, with Cyclin A2 playing a key role in DNA replication and cell division, while Cyclin A1 is mainly expressed in spermatocytes and leukemia cells, and is vital for male meiosis but unnecessary for female meiosis.
- - Research indicates that Cyclin A1 disrupts cell cycle processes in oocytes, causing anaphase arrest due to its inability to be effectively targeted by the APC/C and proteasome, which inhibits separase and prevents proper chromosome segregation.
- - The findings highlight that Cyclin A1 has a dual impact: it is essential for male germ cell division, but its presence in
Optimal culture conditions are essential for successful IVM of mammalian oocytes and for their further development into an embryo. In the present study we used live cell imaging microscopy to assess the effects of suboptimal culture temperature on various aspects of IVM, including duration of meiosis I, dynamics of polar body extrusion, chromosome congression, anaphase-promoting complex/cyclosome (APC/C) activation and aneuploidy. The data showed that even a small deviation from the optimal incubation temperature causes marked changes in the duration and synchronicity of meiosis, APC/C activity and the frequency of chromosome congression and segregation errors.
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