Publications by authors named "Jit Hin Tan"
Introduction: Residual pluripotent stem cells (PSC) within differentiated populations are problematic because of their potential to form tumors. Simple methods to reduce their occurrence are needed.
Methods: Here, we demonstrate that control of the oxygen partial pressure (pO) to physiological levels typical of the developing embryo, enabled by culture on a highly oxygen permeable substrate, reduces the fraction of PSC within and the tumorigenic potential of differentiated populations.
The effects of low oxygen on self-renewal and differentiation of embryonic stem cells.
Curr Opin Organ Transplant
December 2009
Purpose Of Review: To summarize recent reports on the effects of low oxygen on the undifferentiated phenotype and differentiation of embryonic stem cells (ESCs).
Recent Findings: The oxygen level to which ESCs are exposed is an important environmental parameter. Under conditions maintaining the undifferentiated phenotype, low oxygen reduces spontaneous differentiation of human ESCs but reduces pluripotency gene expression in mouse ESCs, although reports are conflicting.