Computational identification of key residues regulating fluorescence emission in a red/green cyanobacteriochrome.

Cyanobacteriochromes (CBCRs) are linear tetrapyrrole bilin-binding photoreceptors of cyanobacteria that exhibit high spectral diversity, gaining attention in optogenetics and bioimaging applications. Several engineering studies on CBCRs were attempted, especially for designing near-infrared (NIR) fluorescent proteins with longer fluorescence wavelengths. However, despite continuous efforts, a key component regulating fluorescence emission property in CBCRs is still poorly understood.

Domain-wise dissection of thermal stability enhancement in multidomain proteins.

Stability is critical for the proper functioning of all proteins. Optimization of protein thermostability is a key step in the development of industrial enzymes and biologics. Herein, we demonstrate that multidomain proteins can be stabilized significantly using domain-based engineering followed by the recombination of the optimized domains.

sp. nov. isolated from coastal seawater.

A Gram-stain-negative, aerobic, yellow-pigmented and non-motile rod-shaped bacterium, designated as GrpM-11, was isolated from coastal seawater collected from the East Sea, Republic of Korea. Strain GrpM-11 could grow at 10-40 °C (optimum, 35 °C), at pH 5.5-9.

sp. nov. isolated from deep-sea water.

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterial strain, designated strain DSW4-44, was isolated from deep-sea water from the East Sea of Korea. The optimum temperature and pH for growth were 25 °C and pH 7.5.

Zhongshania marina sp. nov., isolated from deep-sea water.

A Gram-stain-negative, aerobic, catalase-positive, oxidase-positive, motile by a single polar flagellum, rod-shaped strain, designated DSW25-10, was isolated from the deep-sea water of the East Sea, Republic of Korea. Strain DSW25-10grew at 4-35 °C (optimum, 20-35 °C), at pH 5.5-9.

Pseudoxanthomonas putridarboris sp. nov. isolated from rotten tree.

A Gram-staining-negative, rod-shaped, motile bacterium, designated WD12T, was isolated from a rotten tree at Chungbuk National University, South Korea. WD12T grew optimally at 30-37 °C and pH 7.0-7.