Development of the Static and Dynamic Gene Expression Regulation Toolkit in .

The advancement of metabolic engineering and synthetic biology has promoted in-depth research on the nonmodel microbial metabolism, and the potential of nonmodel organisms in industrial biotechnology is becoming increasingly evident. The nonmodel organism is a safe plant growth promoting bacterium for the production of phenazine compounds; however, its application is seriously hindered due to the lack of an effective gene expression precise regulation toolkit. In this study, we constructed a library of 108 promoter-5'-UTR (PUTR) and characterized them through fluorescent protein detection.

Thin head atomic force microscope for integration with optical microscope.

We present a novel thin head atomic force microscope (AFM) that can be easily integrated with an upright optical microscope (OM). The optical beam detection unit in the AFM used an obliquely incident laser beam onto the cantilever, reducing the AFM head's effective thickness to 7.3 mm.

Calibration of position and orientation for point light source synchronously with single image in photometric stereo.

In this paper, a calibration method for non-isotropic point light source is developed, which is capable of calibrating position and orientation of the point light source with a single image synchronously. Based on the bidirectional reflectance distribution function (BRDF), the relationship between the shape of observed surface and the recorded grayscale image is firstly investigated in a more accurate way. Based on this model, a cost function is proposed to evaluate the estimation error, and a new cumulative, error-free iteration method is designed to recursively estimate the point light source parameters.