Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency.

Immunohistochemistry (IHC) is a widely used technique in diagnostic pathology and biomedical research, but there is still a need to shorten the operation process and reduce the cost of antibodies. This study aims to assess a novel IHC technique that incorporates mechanical microvibration (MMV) to expedite the process, reduce antibody consumption, and enhance staining quality. MMV was generated using coin vibration motors attached to glass slides mounted with consecutive tissue sections.

Automated Lung and Colon Cancer Classification Using Histopathological Images.

Cancer is the leading cause of mortality in the world. And among all cancers lung and colon cancers are 2 of the most common causes of death and morbidity. The aim of this study was to develop an automated lung and colon cancer classification system using histopathological images.

Synergistic effect of glutathione and IgG4 in immune evasion and the implication for cancer immunotherapy.

Background: We recently reported a novel IgG4-centered immune evasion mechanism in cancer, and this was achieved mostly through the Fc-Fc reaction of increased IgG4 to cancer-bound IgG in cancer microenvironment. The mechanism was suggested to be related to cancer hyperprogressive disease (HPD) which is a side-effect often associated to IgG4 subtype PD-1 antibody immunotherapy. HPD was reported to occur in cancers with certain mutated genes including KRAS and such mutations are often associated to glutathione (GSH) synthesis.

Oxidation of Hypophosphorous Acid by a Ruthenium(VI) Nitrido Complex in Aqueous Acidic Solution. Evidence for a Proton-Coupled N-Atom Transfer Mechanism.

The oxidation of hypophosphorous acid (HPO) by a ruthenium(VI) nitrido complex, [(L)Ru(N)(OH)] (; L = ,'-bis(salicylidene)--cyclohexyldiamine dianion), has been studied in aqueous acidic solutions at pH 0-2.50. The reaction has the following stoichiometry: 2[(L)Ru(N)(OH)] + 3HPO + HO → 2[(L)Ru(NHP(OH))(OH)] + HPO.

Gene is Associated With Sex Determination in .

In the past decade, progress has been made in sex determination mechanism in . However, genes responsible for sexual differentiation and its mechanism in remain unknown. Here, we identify a sex determination candidate gene coding adenine phosphoribosyl transferase 3 () in .

Integration of Eye-Centered and Landmark-Centered Codes in Frontal Eye Field Gaze Responses.

The visual system is thought to separate egocentric and allocentric representations, but behavioral experiments show that these codes are optimally integrated to influence goal-directed movements. To test if frontal cortex participates in this integration, we recorded primate frontal eye field activity during a cue-conflict memory delay saccade task. To dissociate egocentric and allocentric coordinates, we surreptitiously shifted a visual landmark during the delay period, causing saccades to deviate by 37% in the same direction.

Cytological and transcriptome analysis reveal that interaction at Sb pollen sterility locus cause down-regulation of important meiosis-related genes associated with high pollen sterility in autotetraploid rice hybrids.

Polyploidy could increase the interactions of pollen sterility loci and Sb locus interaction cause higher pollen abortion than other loci. Therefore, we focused on the interaction at Sb pollen sterility locus in autotetraploid rice compared to diploid rice hybrid using the near-isogenic lines in the present study. Cytological observations indicated that interaction at Sb locus cause high pollen sterility (69.

Pervasive interactions of Sa and Sb loci cause high pollen sterility and abrupt changes in gene expression during meiosis that could be overcome by double neutral genes in autotetraploid rice.

Background: Intersubspecific autotetraploid rice hybrids possess high hybrid vigor; however, low pollen fertility is a critical hindrance in its commercial utilization. Our previous study demonstrated that polyploidy could increase the multi-loci interaction and cause high pollen abortion in autotetraploid rice hybrids. However, there is little known about the critical role of pollen sterility locus or loci in the intersubspecific hybrids.

Effect of allocentric landmarks on primate gaze behavior in a cue conflict task.

The relative contributions of egocentric versus allocentric cues on goal-directed behavior have been examined for reaches, but not saccades. Here, we used a cue conflict task to assess the effect of allocentric landmarks on gaze behavior. Two head-unrestrained macaques maintained central fixation while a target flashed in one of eight radial directions, set against a continuously present visual landmark (two horizontal/vertical lines spanning the visual field, intersecting at one of four oblique locations 11° from the target).