Publications by authors named "Jiri Veleminsky"
Article Synopsis
- The gene construct was cloned into a Potato X potexvirus (PVX) vector and expressed in plants via Agrobacterium tumefaciens inoculation.
- Enhanced expression levels of the recombinant protein were observed in transgenic plants, especially when co-infected with Potato virus Y(O) (PVY(O)), compared to plants expressing the recombinant alone.
We applied the alkaline version of the single-cell gel electrophoresis (comet) assay to roots and leaves of tobacco (Nicotiana tabacum var. xanthi) seedlings or isolated leaf nuclei treated with: (1) the alkylating agent ethyl methanesulphonate, (2) necrotic heat treatments at 50 degrees C, and (3) DNase-I. All three treatments induced a dose-dependent increase in DNA migration, expressed as percentage of tail DNA.
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- Vaccine strategies to treat cervical cancer focus on the HPV16 E7 oncoprotein, enhanced by fusing it with other genes for better immune response.
- Researchers linked a specific segment of the E7 gene to a coat protein from potato virus A, creating new fusion proteins that formed virus-like particles in cells.
- These fusion proteins provided some protective effects against tumor development in mice and showed similar antitumor benefits as other previously constructed vaccines, without significant antibody responses detected.