Publications by authors named "Jiri Frantisek Potuznik"

RNA capping is a prominent RNA modification that influences RNA stability, metabolism, and function. While it was long limited to the study of the most abundant eukaryotic canonical mG cap, the field recently went through a large paradigm shift with the discovery of non-canonical RNA capping in bacteria and ultimately all domains of life. The repertoire of non-canonical caps has expanded to encompass metabolite caps, including NAD, FAD, CoA, UDP-Glucose, and ADP-ribose, alongside alarmone dinucleoside polyphosphate caps, and methylated phosphate cap-like structures.

View Article and Find Full Text PDF
Article Synopsis
  • NAD (Nicotinamide adenine dinucleotide) is essential for cellular metabolism and acts as an RNA cap, but its role in this function is not fully understood.
  • Research on HIV-1-infected cells showed that certain small nuclear and nucleolar RNAs lost their NAD cap, which could affect their stability.
  • By increasing the enzyme that removes the NAD cap, researchers found that HIV-1 infectivity increased, indicating that NAD capping might hinder the virus's ability to replicate.
View Article and Find Full Text PDF

The recent expansion of the field of RNA chemical modifications has changed our understanding of post-transcriptional gene regulation. Apart from internal nucleobase modifications, 7-methylguanosine was long thought to be the only eukaryotic RNA cap. However, the discovery of non-canonical RNA caps in eukaryotes revealed a new niche of previously undetected RNA chemical modifications.

View Article and Find Full Text PDF

Chemical modifications of viral RNA are an integral part of the viral life cycle and are present in most classes of viruses. To date, more than 170 RNA modifications have been discovered in all types of cellular RNA. Only a few, however, have been found in viral RNA, and the function of most of these has yet to be elucidated.

View Article and Find Full Text PDF