Physiological responses to honeybee venom poisoning in a model organism, the firebug Pyrrhocoris apterus.

In this study, the biochemical and physiological features of the firebug Pyrrhocoris apterus were investigated to understand the impact of the honeybee Apis mellifera venom on them using physiological methods (mortality, total level of metabolism), biochemical methods (ELISA, mass spectrometry, polyacrylamide gel electrophoresis, spectrophotometry) and molecular methods (real-time PCR). Together, the obtained findings suggest that venom injection increased the level of adipokinetic hormone (AKH) in the CNS of P. apterus, indicating that this hormone plays a key role in activating defence responses.

Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. parasitisation and associated factors reveals changes impairing winter bee generation.

The extensive annual loss of honey bees (Apis mellifera L.) represents a global problem affecting agriculture and biodiversity. The parasitic mite Varroa destructor, associated with viral co-infections, plays a key role in this loss.

H NMR Profiling of Honey Bee Bodies Revealed Metabolic Differences between Summer and Winter Bees.

In temperate climates, honey bee workers of the species have different lifespans depending on the seasonal phenotype: summer bees (short lifespan) and winter bees (long lifespan). Many studies have revealed the biochemical parameters involved in the lifespan differentiation of summer and winter bees. However, comprehensive information regarding the metabolic changes occurring in their bodies between the two is limited.

Corrigendum: American Foulbrood in the Czech Republic: ERIC II Genotype of Is Prevalent.

[This corrects the article DOI: 10.3389/fvets.2021.

American Foulbrood in the Czech Republic: ERIC II Genotype of Is Prevalent.

American foulbrood (AFB) is a dangerous disease of honeybees () caused by the spore-forming bacterium . According to the ERIC (enterobacterial repetitive intergenic consensus) classification, five genotypes are distinguished, i.e.

Can Be Effectively and Economically Detected Using Hive Debris and Conventional PCR.

European foulbrood (EFB) is an infectious disease of honey bees caused by the bacterium . A method for DNA isolation and conventional PCR diagnosis was developed using hive debris, which was non-invasively collected on paper sheets placed on the bottom boards of hives. Field trials utilized 23 honey bee colonies with clinically positive symptoms and 21 colonies without symptoms.

Winter honeybee () populations show greater potential to induce immune responses than summer populations after immune stimuli.

In the temperate climates of central Europe and North America, two distinct honeybee () populations are found in colonies: short-living summer bees emerge in spring and survive until summer, whereas long-living winter bees emerge in late August and overwinter. Besides the difference in their life spans, each of these populations fulfils a different role in the colonies and individual bees have distinct physiological and immunological adaptations depending on their roles. For instance, winter worker bees have higher vitellogenin levels and larger reserves of nutrients in the fat body than summer bees.

Does the Pollen Diet Influence the Production and Expression of Antimicrobial Peptides in Individual Honey Bees?

We investigated the importance of protein nutrition for honey bee immunity. Different protein diets (monofloral pollen of spp., spp.

A sensitive quantification of the peptide apidaecin 1 isoforms in single bee tissues using a weak cation exchange pre-separation and nanocapillary liquid chromatography coupled with mass spectrometry.

Apidaecins represent an important group of antimicrobial peptides occurring in honey bee hemolymph, where they play an important role as key components of humoral immunity. The present study demonstrates the development of a highly sensitive assay for apidaecin 1 isoforms quantification in the hemolymph or body parts from honey bee individuals. The analytical protocol comprises apidaecins 1 purification and enrichment steps by weak cation-exchange chromatography (WCX) in laboratory-made WCX-Tip microcolumns combined with a desalting step on a reversed-phase sorbent (C8) carried in StageTips.