Enhancement of Growth, Antioxidant Activity, and Immunity in Nile Tilapia () Through Recombinant Expressing L-Gulonolactone Oxidase.

Due to its lack of the L-gulonolactone oxidase () enzyme, Nile tilapia is unable to synthesize vitamin C; thus, it requires an adequate level of exogenous vitamin C in its diet. To enhance antioxidant properties and vitamin C-related effects, we employed recombinant technology to integrate the -encoding gene into the chromosome. In this study, fish were divided into four groups: those fed with a basal diet (CON), a basal diet + vitamin C (VC), a basal diet + wild-type (BS), and a basal diet + recombinant (BS+GULO).

Isolation of Potential Probiotic spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia.

This study aimed to investigate the potential probiotic spp. from the intestine of Nile tilapia in order to construct a recombinant probiotic for the enhancement of the Nile tilapia immune response. One hundred bacterial isolates from the intestine of Nile tilapia were characterized for species identification using the 16s ribosomal RNA (rRNA).