The role of Maillard reaction in increasing affinity between soybean protein isolate and phloretin and its effects on protein functionality.

This study investigated the effects of the Maillard reaction on the interaction between soybean protein isolate (SPI) and phloretin (PHL), along with its impact on the functional properties of soybean protein isolate. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results showed that sodium alginate (SA) was successfully grafted onto SPI. The fluorescence results indicated that the red shift and fluorescence burst of the ternary complexes were more pronounced, indicating that the proteins in the complexes had a more compact tertiary structure.