Design of a water-soluble transmembrane receptor kinase with intact molecular function by QTY code.

Membrane proteins are critical to biological processes and central to life sciences and modern medicine. However, membrane proteins are notoriously challenging to study, mainly owing to difficulties dictated by their highly hydrophobic nature. Previously, we reported QTY code, which is a simple method for designing water-soluble membrane proteins.

Metabolic engineering of Geobacillus thermoglucosidasius for polymer-grade lactic acid production at high temperature.

The production and application of biodegradable polylactic acid are still severely hindered by the cost of its polymer-grade lactic acid monomers. High-temperature biomanufacturing has emerged as an increasingly attractive approach to enable low-cost and high-efficiency bulk chemical production. In this study, thermophilic Geobacillus thermoglucosidasius was reprogrammed to obtain optically pure l-lactic acid- and d-lactic acid-producing strains, G.

Genomic Insights on the Carbon-Negative Workhorse: Systematical Comparative Genomic Analysis on 56 Strains.

, a type of ancient photosynthetic cyanobacteria, is crucial in modern carbon-negative synthetic biology due to its potential for producing bioenergy and high-value products. With its high biomass, fast growth rate, and established genetic manipulation tools, has become a research focus in recent years. Abundant germplasm resources have been accumulated from various habitats, including temperature and salinity conditions relevant to industrialization.

Characterization of the Pro101Gln mutation that enhances the catalytic performance of T. indicus NADH-dependent d-lactate dehydrogenase.

NADH-dependent d-lactate dehydrogenases (d-LDH) are important for the industrial production of d-lactic acid. Here, we identify and characterize an improved d-lactate dehydrogenase mutant (d-LDH1) that contains the Pro101Gln mutation. The specific enzyme activities of d-LDH1 toward pyruvate and NADH are 21.

Activating a dormant metabolic pathway for high-temperature l-alanine production in .

l-Alanine is an important amino acid widely used in food, medicine, materials, and other fields. Here, we develop as an efficient l-alanine microbial cell factory capable of realizing high-temperature fermentation. By enhancing the glycolytic pathway, knocking out the by-product pathways and overexpressing the thermostable alanine dehydrogenase, the engineered .

Eliminating host-guest incompatibility via enzyme mining enables the high-temperature production of -acetylglucosamine.

The host-guest incompatibility between a production host and non-native enzymes has posed an arduous challenge for synthetic biology, particularly between mesophile-derived enzymes and a thermophilic chassis. In the present study, we develop a thermophilic enzyme mining strategy comprising an automated co-evolution-based screening pipeline (http://cem.sjtu.

Microbial cell factories for bio-based biodegradable plastics production.

The misuse of petroleum-based plastics has resulted in serious environmental pollution and resource wastage. Biodegradable plastics can be used as green substitutes for traditional plastics. Here, we discuss the feasibility and technical bottlenecks in developing microbial cell factories for the production of biodegradable plastics from lignocellulosic wastes.

Kinetic characteristics of long-term repeated fed-batch (LtRFb) l-lactic acid fermentation by a strain.

Application of degradable plastics is the most critical solution to plastic pollution. As the precursor of biodegradable plastic PLA (polylactic acid), efficient production of l-lactic acid is vital for the commercial replacement of traditional plastics. H-2, a robust strain, was investigated for effective production of l-lactic acid using long-term repeated fed-batch (LtRFb) fermentation.

Steps Toward High-Performance PLA: Economical Production of d-Lactate Enabled by a Newly Isolated Sporolactobacillus terrae Strain.

Optically pure d-lactate production has received much attention for its critical role in high-performance polylactic acid production. However, the current technology can hardly meet the comprehensive demand of industrialization on final titer, productivity, optical purity, and raw material costs. Here, an efficient d-lactate producer strain, Sporolactobacillus terrae (S.