Survey of Incidence and Nested PCR Detection of Sugarcane White Leaf in Different Varieties.

Sugarcane white leaf (SCWL) is a devastating sugarcane () disease caused by a 16SrXI group phytoplasma, which is extremely harmful to sugarcane production. To determine the occurrence of SCWL in different varieties in 2018, we conducted a field survey and performed nested PCR detection of SCWL phytoplasma in cane-planting areas of Mangweng and Hepai in Gengma, Yunnan province, which are the areas most severely affected by SCWL in China. The results of the field survey showed that the symptomatic incidence of SCWL differed among varieties.

Group 16SrXI phytoplasma strains, including subgroup 16SrXI-B and a new subgroup, 16SrXI-D, are associated with sugar cane white leaf.

Sugar cane white leaf (SCWL) is a serious disease caused by phytoplasmas. In this study, we performed nested PCR with phytoplasma universal primer pairs (P1/P7 and R16F2n/R16R2) for the 16S rRNA gene to detect SCWL phytoplasmas in 31 SCWL samples collected from Baoshan and Lincang, Yunnan, China. We cloned and sequenced the nested PCR products, revealing that the 16S rRNA gene sequences from 31 SCWL samples were all 1247 bp in length and shared more than 99 % nucleotide sequence similarity with the 16S rRNA gene sequences of SCWL phytoplasmas from various countries.

The association between CD14 gene C-260T polymorphism and coronary heart disease risk: a meta-analysis.

Monocyte differentiation antigen CD14 is considered an important cell-activating mediator of inflammatory responses that may result in atherosclerosis, coronary heart disease (CHD), thrombus formation, and myocardial infarction (MI). A common C-260T polymorphism in the promoter of the CD14 gene, the trans-membrane receptor of lipopolysaccharides, has been inconsistently associated with CHD. To investigate this inconsistency, we performed a meta-analysis of 28 studies involving a total of 13,335 CHD cases and 7,979 controls for C-260T of the CD14 gene to evaluate the effect of CD14 on genetic susceptibility for CHD.

[Survey on HIV/AIDS-related knowledge and behavior of high risk population in Taiyuan City].

Objective: To understand the status of HIV/AIDS-related knowledge and behavior of high risk population in Taiyuan City, and to explore the correlation between the two factors.

Methods: The questionnaires were designed according to the questionnaire for monitoring the behavior of HIV/AIDS-related high risk population in China, the basic knowledge, high-risk behavior and so on were included in the questionnaires. A random cluster sampling and stratified sampling method was used to interview with commercial sex workers, drug users and MSM (men who have sex with men) in Taiyuan City.

Prevalence and distribution of cognitive impairment no dementia (CIND) among the aged population and the analysis of socio-demographic characteristics: the community-based cross-sectional study.

The epidemiology on "cognitive impairment no dementia" (CIND) and its natural history are of great importance for understanding the transition from normal aging to dementia. Epidemiologic studies of CIND, however, are limited in China. The goal of our study was to determine the prevalence and distribution of CIND in the aged population and analyze socio-demographic factors.

[A case-control study on the influencing factors to mild cognitive impairment among the community-based elderly population].

Objective: To explore the influencing factors on mild cognitive impairment among the community-based elderly population.

Methods: A 'n : m' matched case-control study was conducted to analyze the risk factors. Cox regression model of survival analysis was selected to deal with non-geometric proportional matched data which was difficult to analyze by logistic regression model.