Transcription Factor CEBPD-Mediated WTAP Facilitates the Stemness, Growth, Migration and Glycolysis of Glioblastoma Stem Like Cells.

Glioblastoma stem like cells (GSCs) are a group of cells with strong tumorigenicity that exist in glioblastoma (GBM). Wilms tumor 1-associated protein (WTAP) is thought to promote the malignant process of GBM. However, whether WTAP regulates GSCs function to mediate GBM process is still unclear.

Methylation mediated silencing of miR-23b expression and its role in glioma stem cells.

MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate gene expression at a post-transcriptional level. Some miRNAs harboring CGIs undergo methylation mediated silencing, a characteristic of many tumor suppressor genes. To identify such miRNAs in glioma stem cells (GSCs), we first showed that miR-23b is frequently methylated in GSCs but not in parallel U87 cells.

Combination of tamoxifen and antisense human telomerase RNA inhibits glioma cell proliferation and anti-apoptosis via suppression of telomerase activity.

Accumulating evidence indicates that telomerase activity and human telomerase RNA (hTR) play a potentially crucial role in maintaining the malignant progression of human gliomas. Tamoxifen (TAM) induces cell growth inhibition by modulating several cellular activities, including signaling pathways and the cell cycle. In this study, we aimed to evaluate the effect of combination therapy with TAM and antisense hTR on malignant glioma growth in vitro.

Bone morphogenetic protein 4 inhibits cell proliferation and induces apoptosis in glioma stem cells.

Glioma stem cells (GSCs), which are originated from transformed neural stem cells, are tumor-initiating cells of glioma, the most common primary malignant neoplasm of the central nervous system. Extensive studies have shown that bone morphogenetic protein 4 (BMP4) plays an important role in the differentiation and proliferation of neural stem cells. To seek the functions and mechanisms of BMP4 in GSCs, GSCs isolated from U87 human glioma cells by using vincristine were exposed to BMP4 protein.