A 96-well microplate-based protein microarray chip which can simultaneously detect three different families of antibiotics using a multiplexed approach has been developed for the first time. All steps in the microarray chip can be completed through an automated biochip analyzer, enabling high throughput analysis of 96 samples within 60 min according to the set program. The microarray chip can be visually evaluated by the color depth of array points, and can also be quantified through a scanner.
View Article and Find Full Text PDFThe aim of this study was to examine the components of the cell-free supernatant (CFS) derived from a novel strain of psychrophilic , , and to further elucidate the impact of this CFS on various cellular processes. Specifically, we sought to understand its effects on the cell membrane, protein and DNA release, protease activity, and metabolites of and , thereby clarifying the antibacterial mechanism involved. The CFS components were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS), the Coomassie Brilliant Blue method, and the phenol-sulfuric acid method.
View Article and Find Full Text PDFPoly(malic acid) (PMLA) as one of the important metabolites in Aureobasidium pullulans has great application in a variety of fields. LaeA, a global regulator capable of controlling fungal secondary metabolites, has been identified in A. pullulans.
View Article and Find Full Text PDFJX313 is an extremely halophilic archaea that can grow in a NaCl-saturated environment. The excellent salt tolerance of makes it a high-potential candidate for researching the salt stress mechanisms of halophilic microorganisms from . In this study, transcriptome analysis revealed that three genes related to the biosynthesis of vitamin B were upregulated in response to salt stress.
View Article and Find Full Text PDF2-bromoacetamide (BAcAm) is an emerging class of unregulated disinfection by-products (DBPs), with potent cytogenetic and developmental toxicity in animals. However, whether BAcAm exerts toxic effects on mammalian oocyte quality remains to be elucidate. In this research, we investigated the effect of BAcAm on mouse and human oocyte maturation with an in vitro culture system.
View Article and Find Full Text PDF6A7 is a low-temperature yeast strain that can produce lipases. Yeast, which is made up of chassis cells, is an important part of synthetic biology, and the use of the lipase-producing properties of 6A7 for the production of fatty acids provides a new pathway for targeted synthesis in yeast cell factories. In this study, we performed RNA-seq on lipase-producing 6A7 at different temperatures (15 °C, 20 °C, 20 °C without corn oil, and 25 °C).
View Article and Find Full Text PDFIn Brief: Post-ovulatory aging (POA) results in a decline in oocyte quality and embryonic developmental capacity although the underlying mechanisms remain elusive. This study provides comprehensive mRNA expression profiles of fresh and aging oocytes in mice for the first time.
Abstract: POA impairs the quality of mammalian oocytes with harmful effects on the developmental potential of the embryo.
The Na/H antiporter NhaC family protein is a kind of Na/H exchanger from the ion transporter (IT) superfamily, which has mainly been identified in the halophilic bacteria of . However, little is known about the Na/H antiporter NhaC family of proteins in the extremely halophilic archaea. In this study, two Na/H antiporter genes, and , were screened from the genome of based on the gene library and complementation of salt-sensitive KNabc.
View Article and Find Full Text PDF3-Hydroxypropionic acid (3-HP) is an important intermediate compound in the chemical industry. Green and environmentally friendly microbial synthesis methods are becoming increasingly popular in a range of industries. Compared to other chassis cells, possesses advantages, such as high tolerance to organic acid and a sufficient precursor required to synthesize 3-HP.
View Article and Find Full Text PDF2-bromoacetamide (BAcAm), a new class of disinfection by-products (DBPs), is widely detected in drinking water across the world. Reports of the high cytogenetic toxicity of BAcAm have aroused public attention concerning its toxic effects on early embryonic development. In this study, we optimized an in vitro culture (IVC) system for peri- and early post-implantation mouse embryos and used this system to determine the developmental toxicity of BAcAm.
View Article and Find Full Text PDFChaetoglobosin A (CheA), a well-known macrocyclic alkaloid with prominently highly antimycotic, antiparasitic, and antitumor properties, is mainly produced by . However, a limited understanding of the transcriptional regulation of CheA biosynthesis has hampered its application and commercialization in agriculture and biomedicine. Here, a comprehensive study of the gene, which encodes a basic helix-loop-helix family regulator with a putative role in the regulation of fungal growth and CheA biosynthesis, was performed by employing -disruption and -complementation strategies in the biocontrol species .
View Article and Find Full Text PDFBackground: In mammals, oocytes display compromised quality after experiencing a process of postovulatory aging. However, the mechanisms underlying are not yet fully understood. Here, we portrayed a protein expression profile of fresh and aging metaphase II (MII) mouse oocytes by means of four-dimensional label-free quantification mass spectrometry (4D-LFQ).
View Article and Find Full Text PDFJ Assist Reprod Genet
November 2022
Purpose: Zona pellucida-free (ZP-free) embryos often fail to achieve good developmental outcomes and are routinely discarded in assisted reproductive laboratories. Existing attempts to rescue ZP-free embryos are not widely used due to operational complexity and high technical requirements. To handle cases with missing ZP, we applied modified sodium hyaluronate gel (MSHG) to embryo culture to determine if it can function as a substitute for human zona pellucida.
View Article and Find Full Text PDFTranslational regulation plays a critical role during the oocyte-to-embryo transition (OET) and zygotic genome activation (ZGA). Here, we integrated ultra-low-input ribosome profiling (Ribo-lite) with messenger RNA sequencing to co-profile the translatome and transcriptome in human oocytes and early embryos. Comparison with mouse counterparts identified widespread differentially translated gene functioning in epigenetic reprogramming, transposon defense, and small RNA biogenesis, in part driven by species-specific regulatory elements in 3' untranslated regions.
View Article and Find Full Text PDFPullulan and melanin have become important secondary metabolites that are now widely studied. In this study, a strain of Aureobasidium pullulans HIT-LCY3 was used to ferment potato starch industrial waste to produce pullulan and melanin. After optimization, the culture conditions for the fermentation medium were obtained: inoculum age of 48 h, initial pH of 6.
View Article and Find Full Text PDFThe global regulator LaeA has been confirmed to govern the production of secondary metabolites in fungi. Herein, we examined the role of LaeA in Chaetomium globosum. Similarly as observed in other filamentous, CgLaeA had a significant effect on the secondary metabolism.
View Article and Find Full Text PDFCytochalasins are a group of fungal secondary metabolites with diverse structures and bioactivities, including chaetoglobosin A production. Chaetoglobosin A is produced by Chaetomium globosum and has potential antifungal activity. Bioinformatics analysis of the chaetoglobosin A gene cluster (che) showed it that consists of nine open reading frames, including those encoding polyketide synthases (PKSs), PKS extender units, post-PKS modifications, and proposed regulators.
View Article and Find Full Text PDFA protocol of visible-light-promoted C2 selective arylation of quinoline and pyridine -oxides, with diaryliodonium tetrafluoroborate as an arylation reagent, using eosin Y as a photocatalyst for the construction of -heterobiaryls was presented. This methodology provided an efficient way for the synthesis of 2-aryl-substituted quinoline and pyridine -oxides. This strategy has the following advantages: specific regioselectivity, simple operation, good functional group tolerance, and high to moderate yields under mild conditions.
View Article and Find Full Text PDFCorynebacterium glutamicum is an important industrial strain used for the production of amino acids and vitamins. Most tools developed for overexpression of genes in C. glutamicum are based on the inducible promoter regulated by the lacI gene or contain an antibiotic resistance gene as a selection marker.
View Article and Find Full Text PDFATP-binding cassette (ABC) transporters are a superfamily of proteins that transport nutrient substances and secondary metabolites through cell membranes. They also act as an uptake system for N,N'-diacetylchitobiose (GlcNAc) in . (GlcNAc) is an important inducer of chitinase.
View Article and Find Full Text PDFFusarium sporotrichioides, is a common soil-borne plant pathogen causing dry rot of potato in Northeast China. The objective of this study was to identify the main antifungal substances from Chaetomium globosum W7 against F. sporotrichioides.
View Article and Find Full Text PDFBraz J Microbiol
March 2018
Chaetoglobosin A is an antibacterial compound produced by Chaetomium globosum, with potential application as a biopesticide and cancer treatment drug. The aim of this study was to evaluate the feasibility of utilizing cornstalks to produce chaetoglobosin A by C. globosum W7 in solid-batch fermentation and to determine an optimal method for purification of the products.
View Article and Find Full Text PDFA novel β-1,3-glucanase gene, designated Ccglu17A, was cloned from the biological control fungus Chaetomium cupreum Ame. Its 1626-bp open reading frame encoded 541 amino acids. The corresponding amino acid sequence showed highest identity (67 %) with a glycoside hydrolase family 17 β-1,3-glucanase from Chaetomium globosum.
View Article and Find Full Text PDFAppl Biochem Biotechnol
December 2016
As alternative microorganisms for butanol production with high butanol tolerant and productivity are in high demand, one excellent butanol-tolerant bacterium, S10, was isolated and identified as Clostridium acetobutylicum S10. In order to enhance the performance of butanol production, organic acids and neutral red were added during butanol fermentation. Synergistic effects were exhibited in the combinations of organic acids and neutral red to promote butanol production.
View Article and Find Full Text PDFRelieving the feedback inhibition of key enzymes in a metabolic pathway is frequently the first step of producer-strain construction by genetic engineering. However, the strict feedback regulation exercised by microorganisms in methionine biosynthesis often makes it difficult to produce methionine at a high level. In this study, Corynebacterium glutamicum ATCC 13032 was metabolically engineered for methionine production.
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