Immunosuppressive Regulation of Dendritic Cells and T Cells in Allergic Rhinitis by Semaphorin 3A.

Background: Semaphrin3A (Sema3A) was found to play a major role in immune regulation in autoimmune diseases and to be of importance in allergic disease. However, the effect of Sema3A on allergic rhinitis (AR) is not fully clear.

Objective: We sought to elucidate the effects of Sema3A on the regulation of dendritic cells (DCs) and naive CD4 T cells in AR.

Cholinergic neuron-like D-U87 cells promote polarization of allergic rhinitis T-helper 2 cells.

Background: Parasympathetic nerve hypersensitivity contributes to the severity of allergic rhinitis (AR), but the precise mechanism underlying neuroimmune regulation in patients with AR remains unclear. This study investigated the effect of cholinergic nerve inhibition on AR CD4 T-helper (Th)2-cell polarization and the underlying regulatory mechanism in vitro.

Methods: An in-vitro neuroimmune coculture model of D-U87 cells and CD4 T cells was established.

Differentiation of human glioblastoma U87 cells into cholinergic neuron.

To facilitate research methodologies for investigating the role of cholinergic nerves in many diseases, establishing an in vitro cholinergic neuron model is necessary. In this study, we investigated whether human glioblastoma U87 cells could be differentiated into cholinergic neurons in vitro. Sodium butyrate was used as the differentiation agent.