Versatile, reusable and highly sensitive SERS-based point-of-care testing microplatform for reliable ATP detection.

The advancement in miniaturized Raman spectrometers, coupled with the single-molecule-level sensitivity and unique fingerprint identification capability of surface-enhanced Raman scattering (SERS), offers great potential for point-of-care testing (POCT). Despite this, accurately quantifying analyte molecules, particularly in complex samples with limited sample volumes, remains difficult. Herein, we present a versatile and reusable SERS microplatform for highly sensitive and reliable quantitative detection of adenosine triphosphate (ATP) in biological fluids.

Monolithic 3D structural-substrate SERS sensing platform for ultrasensitive and highly-specific analysis of trace bisphenol A.

Constructing advanced substrates with excellent features is promising for sensitive surface-enhanced Raman spectroscopy (SERS) detection. Here a novel capillary monolithic 3D structural-substrate SERS platform with Au@cDNA@Ag@Cyanine 3-aptamer nanoparticles (Au@cDNA@Ag@Cy3-Apt NPs) was fabricated for rapid, highly specific profiling of ultra-trace Bisphenol A (BPA). The proposed SERS platform combined both in-capillary SERS and aptamer-affinity recognition strategies, in which the superior SERS properties of Au-Ag NPs, aptamer selectivity, and the advantages of capillary monolith were integrated.

Online specific recognition of mycotoxins using aptamer-grafted ionic affinity monolith with mixed-mode mechanism.

Herein, a facile and practical aptamer-grafted ionic affinity monolith with mixed-mode mechanism was explored as a versatile platform for online specific recognition of polar and non-polar mycotoxins. The mixed-mode mechanism including molecular affinity adsorption (between aptamers and targets), hydrophilic interaction and ionic interaction (between stationary phase and targets) were adopted and provided a better flexibility in adjusting separation selectivity to reduce nonspecific adsorption with respect to the single mode. Preparation and characterization of aptamer-based affinity monoliths were investigated, The characterization of pore size distribution, Brunauer-Emmett-Teller (BET) surface area and the specificity and cross-reaction were also evaluated.

Three dimensional graphene materials doped with heteroatoms for extraction and adsorption of environmental pollutants in wastewater.

Environmental pollution by heavy metal ions, organic pollutants, oils, pesticides or dyes is a ubiquitous problem adversely affecting human health and environmental ecology. Development and application novel adsorbents in full-scale treatment systems with effectiveness properties could effective ways to facilitate the extraction and adsorption of environment pollutants from wastewater. Graphene materials have drawn much attention due to their extraordinary electron mobilities, high surface areas, good thermal conductivities, and excellent mechanical properties.

Online high-efficient specific detection of zearalenone in rice by using high-loading aptamer affinity hydrophilic monolithic column coupled with HPLC.

Via the facile photopolymerization and thiol-ene click chemistry, a hydrophilic polyhedral oligomeric silsesquioxane (POSS)-containing affinity monolithic column with a high load of aptamers and fast preparation was presented, and successfully applied to the efficient specific recognition and sensitive detection of zearalenone (ZEN) by coupling with HPLC. Optimization of polymerization reaction and polymer recipe were studied, and characteristics such as structural morphology, affinity performance and specificity to ZEN were evaluated. A highly hydrophilic POSS-based aptamer affinity monolith was rapidly prepared in 1.

Towards high-efficient online specific discrimination of zearalenone by using gold nanoparticles@aptamer-based affinity monolithic column.

Sensitive and specific analysis of zearalenone (ZEN) mycotoxin in cereals for ensuring food safety is critical and remains challenging. Herein, a new gold nanoparticles @aptamer-functionalized hybrid affinity monolithic column was proposed and employed for online specific recognition of ZEN by HPLC. Characterization on the morphology, Brunauer-Emmett-Teller (BET) surface area mechanical stability and specific performance of the obtained affinity monolith were investigated.

A facile AuNPs@aptamer-modified mercaptosiloxane-based hybrid affinity monolith with an unusually high coverage density of aptamer for on-column selective extraction of ochratoxin A.

A convenient and high-performance AuNPs@aptamer-modified mercaptosiloxane-based hybrid affinity monolithic column with an unusually high coverage density of aptamers was facilely prepared and used for on-column selective recognition of ochratoxin A (OTA). Due to the high surface-to-volume ratio of AuNPs, the robust conjugation of Au-SH and large specific surface area of hybrid-silica monolith, high coverage density of 5'-SH-aptamers up to 3494 pmol μL-1 was achieved, which was 2.5-10 folds higher than that of other previously reported affinity monoliths modified with AuNPs@Apt.

Aptamer-based polyhedral oligomeric silsesquioxane (POSS)-containing hybrid affinity monolith prepared via a "one-pot" process for selective extraction of ochratoxin A.

A novel aptamer-based polyhedral oligomeric silsesquioxane (POSS)-containing hybrid affinity monolith has been prepared with a facile "one-pot" process simultaneously via "free radical polymerization" and "thiol-ene" click reaction, and used for on-line selective extraction and practical analysis to trace ochratoxin A (OTA). By using the ternary porogenic mixture composed of water/DMF/PEG, a homogeneous polymerization mixture with POSS chemicals, acrylate-based monomers and aptamer aqueous solution was obtained, and the copolymerization of POSS chemicals, polymer monomers and aptamer aqueous solution was systematically studied. Characterizations such as the morphology, FT-IR and fluorescence spectra, mechanical stability, dynamic binding capacity, cross-reactivity and selectivity of the resultant affinity monolith were also evaluated.