Enhanced Detection of Novel Low-Frequency Gene Fusions via High-Yield Ligation and Multiplexed Enrichment Sequencing.

Panel-based methods are commonly employed for the analysis of novel gene fusions in precision diagnostics and new drug development in cancer. However, these methods are constrained by limitations in ligation yield and the enrichment of novel gene fusions with low variant allele frequencies. In this study, we conducted a pioneering investigation into the stability of double-stranded adapter DNA, resulting in improved ligation yield and enhanced conversion efficiency.

Ultrasound-Driven Piezoelectrocatalytic Immunoactivation of Deep Tumor.

Tumor heterogeneity makes routine drugs difficult to penetrate solid tumors, limiting their therapy efficacies. Based on high tissue penetrability of hydrogen molecules (H ) and ultrasound (US) and the immunomodulation effects of H and lactic acid (LA), this work proposes a novel strategy of US-driven piezoelectrocatalytic tumor immunoactivation for high-efficacy therapy of deep tumors by piezoelectrocatalytic hydrogen generation and LA deprivation. A kind of US-responsive piezoelectric SnS nanosheets (SSN) is developed to realize US-triggered local hydrogen production and simultaneous LA deprivation in deep tumors.

Open-Shell Nanosensitizers for Glutathione Responsive Cancer Sonodynamic Therapy.

Deleterious effects to normal tissues and short biological half-life of sonosensitizers limit the applications of sonodynamic therapy (SDT). Herein, a new sonosensitizer (Cu(II)NS) is synthesized that consists of porphyrins, chelated Cu , and poly(ethylene glycol) (PEG) to overcome the challenges of SDT. As Cu contains 27 electrons, Cu(II)NS has an unpaired electron (open shell), resulting in a doublet ground state and little sonosensitivity.

Nano vanadium dioxide films deposited on biomedical titanium: a novel approach for simultaneously enhanced osteogenic and antibacterial effects.

Vanadium is a trace element in the human body, and vanadium compounds have a promising future in biological and medical applications due to their various biological activities and low toxicity. Herein, a novel pure vanadium dioxide (VO) nanofilm was deposited on a substrate of biomedical titanium by magnetron sputtering. The antibacterial effect of VO against the methicillin-resistant Staphylococcus aureus (MRSA) was validated in vitro and in vivo.

The improvement of corrosion resistance, biocompatibility and osteogenesis of the novel porous Mg-Nd-Zn alloy.

A magnesium scaffold is a promising biodegradable bone repair material. However, its poor corrosion resistance limits its clinical application. In this study, we improved the corrosion resistance, biocompatibility and osteointergration ability of magnesium by alloying it with neodymium (Nd) and zinc (Zn), then fabricated a novel open-porous Mg-Nd-Zn (P-MNZ) alloy using a titanium wire space holder (TWSH) method.