Uptake of Per- and Polyfluoroalkyl Substances into Concrete from Aqueous Film-Forming Foams: Experimental Investigations and Comparison to Field-Impacted Samples.

The widespread use of aqueous film-forming foams (AFFFs) in firefighting has led to significant contamination by per- and polyfluoroalkyl substances (PFAS), including in building materials like concrete. This study investigated the initial phase of PFAS contamination in concrete, focusing on factors influencing PFAS retention and penetration. Laboratory experiments assessed the uptake kinetics of PFAS into concrete over one year, revealing that PFAS penetrated beyond surface layers, as confirmed by high-resolution mass spectrometry and desorption electrospray ionization mass spectrometry imaging.

A novel α-amylase from Streptococcus thermophilus 17140 with β-glycosidic bond hydrolysis capability for the transformation of rare ginsenosides.

Alpha-amylases typically act on starch and oligosaccharides that contain α-1,4 glycosidic linkages, while ginsenosides primarily consist of β-1,2 and β-1,6 glycosidic bonds, which cannot be hydrolyzed by α-amylases. However, for the first time, we have successfully isolated Streptococcus thermophilus 17140 (St17140), capable of converting ginsenoside Rb1 into rare ginsenosides. St17140 expresses a novel α-amylase (StAMY), which hydrolyzes ginsenoside Rb1 to produce Rd, gypenoside XVII, F, and Rh.

Establishment of nasal and olfactory epithelium organoids for unveiling mechanism of tissue regeneration and pathogenesis of nasal diseases.

Organoid is an ideal in vitro model with cellular heterogeneity and genetic stability when passaging. Currently, organoids are exploited as new tools in a variety of preclinical researches and applications for disease modeling, drug screening, host-microbial interactions, and regenerative therapy. Advances have been made in the establishment of nasal and olfactory epithelium organoids that are used to investigate the pathogenesis of smell-related diseases and cellular/molecular mechanism underlying the regeneration of olfactory epithelium.

A signal energy approach of acoustic source localization in plate structures using a discrete sensor array.

In the field of engineering structural health monitoring, acoustic source localization (ASL) is a common method to monitor early damage. Most of the existing ASL techniques have high requirements for accurate acquisition of time of arrival, and require complex iterative algorithms or signal processing techniques, which are not conducive to real-time monitoring. In this paper, a signal energy approach of acoustic source localization in plate structures using a discrete sensor array is proposed.

Mesenchymal stem cell treatment alleviates seawater drowning induced lung injury by inhibiting the TNFα/Snail/EMT pathway.

Background: Seawater drowning (SWD) has been an escalating hazard in recent years. It can not only cause immediate death but can also inflict severe complications, such as acute lung injury (ALI), which greatly increases the mortality rate. Thus, investigating the mechanism of SWD induced lung injury and discovering effective treatments is of great importance.

Zwitterionic, cationic, and anionic PFAS in freshwater sediments from AFFF-impacted and non-impacted sites of Eastern Canada.

Zwitterionic, cationic, and anionic per- and polyfluoroalkyl substances (PFAS) were investigated in freshwater sediments of Canada, including sites impacted by aqueous film-forming foams (AFFFs). The first step of the project involved optimizing the extraction method with equilibrated sediment-water-AFFF samples. The analytical method had acceptable linearity, accuracy, and precision in the sediment matrix, and was further validated with NIST SRM 1936.

Critical Evaluation and Meta-Analysis of Ecotoxicological Data on Per- and Polyfluoroalkyl Substances (PFAS) in Freshwater Species.

Despite the increasing concern regarding the ecological risks posed by per- and polyfluoroalkyl substances (PFAS), a lack of comprehensive understanding of their actual ecotoxicity remains. Through a meticulous examination of 91 peer-reviewed studies investigating effects at a population level and constructing probabilistic species sensitivity distributions (PSSDs), we present a state-of-the-science hazard assessment of PFAS in freshwater species. Using data subsets containing suboptimal data led to an overestimation of the predicted no-effect concentrations (PNECs) of PFAS.

A single-cell transcriptomic census of mammalian olfactory epithelium aging.

Mammalian olfactory epithelium has the capacity of self-renewal throughout life. Aging is one of the major causes leading to the olfactory dysfunction. Here, we performed single-cell RNA sequencing (scRNA-seq) analysis on young and aged murine olfactory epithelium (OE) and identified aging-related differentially expressed genes (DEGs) throughout 21 cell types.

Enzyme Catalyzed Formation of CoA Adducts of Fluorinated Hexanoic Acid Analogues using a Long-Chain acyl-CoA Synthetase from sp. Strain NB4-1Y.

Per and polyfluoroalkyl substances (PFAS) are a large family of anthropogenic fluorinated chemicals of increasing environmental concern. Over recent years, numerous microbial communities have been found to be capable of metabolizing some polyfluoroalkyl substances, generating a range of low-molecular-weight PFAS metabolites. One proposed pathway for the microbial breakdown of fluorinated carboxylates includes β-oxidation, this pathway is initiated by the formation of a CoA adduct.

Aggregation-induced electrochemiluminescence based on intramolecular charge transfer and twisted molecular conformation for label-free Immunoassay.

Organic emitters with exceptional properties exhibit significant potential in the field of aggregation-induced electrochemiluminescence (AIECL); however, their practicality is impeded by limited ECL efficiency (Φ). This paper investigates a novel type of AIECL emitter (BDPPA NPs), where an efficient intramolecular charge transfer (ICT) effect and highly twisted conformation contribute to a remarkable enhancement of ECL. The ICT effect reduces the electron transfer path, while the twisted conformation effectively restricts π-π stacking and intramolecular motions.

The Effect of Attentional Bias on Emotions in Patients with Breast Cancer.

Background: Attentional bias may influence the emotional experiences of breast cancer patients, both positively and negatively. This study aimed to investigate attentional bias in breast cancer patients and its impact on their emotions.

Method: Thirty-eight breast cancer patients completed a modified dot-probe task and the Depression Anxiety and Stress Scale to assess attentional bias and emotional states.

The relationship between psychological distress and cognitive failure among breast cancer survivors: a network analysis.

Background: Psychological distress is highly prevalent and has a severe impact on the quality of life among breast cancer survivors. This type of distress is associated with cognitive failure. However, previous studies have focused solely on the total scale scores of these two concepts while ignoring the unique relationship between specific components.

A damage localization technique using wave front shapes in composite laminates without knowing the velocity profile.

Composite laminates are widely used in various fields, but their structures are prone to cracks and damage. Due to the difference in angles of the instantaneous direction of the wave front propagation and the direction of the energy flow in an anisotropic material, the use of Lamb waves for damage localization in composite laminates is a challenging task. Establishing the wave front shape equation can overcome the difficulty of damage localization caused by anisotropy, but this usually requires a priori knowledge of the acoustic velocity distribution of the laminates, which is not convenient for efficient damage localization.

Fast analysis of short-chain and ultra-short-chain fluorinated organics in water by on-line extraction coupled to HPLC-HRMS.

A rapid on-line solid-phase extraction liquid chromatography high-resolution mass spectrometry (on-line SPE-LC-HRMS) method was developed to analyze 11 ultra-short and short-chain PFAS in surface water. Analytical optimization involved screening 7 chromatographic columns and 5 on-line SPE columns, as well as evaluating SPE loading conditions, filters, sample acidification, chromatographic mobile phases, and SPE loading mobile phases. The optimized method was then applied to 44 river water samples collected in Eastern Canada, including sites near airports with fire-training areas.

Vaporization of perfluorocarbon attenuates sea-water-drowning-induced acute lung injury by deactivating the NLRP3 inflammasomes in canines.

Seawater-drowning-induced acute lung injury (SD-ALI) is a life-threatening disorder characterized by increased alveolar-capillary permeability, an excessive inflammatory response, and refractory hypoxemia. Perfluorocarbons (PFCs) are biocompatible compounds that are chemically and biologically inert and lack toxicity as oxygen carriers, which could reduce lung injury and . The aim of our study was to explore whether the vaporization of PFCs could reduce the severity of SD-ALI in canines and investigate the underlying mechanisms.

Clinical, experimental and pathophysiological effects of Yaq-001: a non-absorbable, gut-restricted adsorbent in models and patients with cirrhosis.

Objective: Targeting bacterial translocation in cirrhosis is limited to antibiotics with risk of antimicrobial resistance. This study explored the therapeutic potential of a non-absorbable, gut-restricted, engineered carbon bead adsorbent, Yaq-001 in models of cirrhosis and acute-on-chronic liver failure (ACLF) and, its safety and tolerability in a clinical trial in cirrhosis.

Design: Performance of Yaq-001 was evaluated .

Species Distribution, Typical Clinical Features and Risk Factors for Poor Prognosis of Super-Elderly Patients with Bloodstream Infection in China.

Objective: Bloodstream infection (BSI) is characterized by high mortality, especially among these increasing super-elderly patients (≥85 years), and this study was conducted to understand the species distribution, typical clinical features and risk factors for poor prognosis of super-elderly patients with BSI.

Methods: Based on previous work, this retrospective study was performed by reviewing an ongoing prospective medical database in a comprehensive tertiary center in China, and all super-elderly patients with BSI in the past 6 years were enrolled in this study.

Results: Out of 5944 adult-patients with BSI, there were totally 431 super-elderly patients (≥85 years old) enrolled in this study and age ≥90 years accounted for 31.

Applying the modified UV-activated TOP assay to complex matrices impacted by aqueous film-forming foams.

Per- and polyfluoroalkyl substances (PFAS) are a large chemical family, and numerous chemical species can co-exist in environmental samples, especially those impacted by aqueous film-forming foams (AFFFs). Given the limited availability of chemical standards, capturing the total amount of PFAS is challenging. Thus, the total oxidizable precursor (TOP) assay has been developed to estimate the total amount of PFAS via the oxidative conversion of precursors into perfluorocarboxylic acids (PFCAs).

Cholesterol metabolism: physiological regulation and diseases.

Cholesterol homeostasis is crucial for cellular and systemic function. The disorder of cholesterol metabolism not only accelerates the onset of cardiovascular disease (CVD) but is also the fundamental cause of other ailments. The regulation of cholesterol metabolism in the human is an extremely complex process.

Near-infrared electrochemiluminescence biosensors facilitated by thermally activated delayed fluorescence (TADF) emitters for ctDNA analysis.

The near-infrared electrochemiluminescence technique (NIR ECL) has gained significant attention as a powerful analytical tool in biomedicine and clinical diagnosis due to its inherent advantages. In this work, we successfully synthesized a novel NIR ECL emitter of TPA-DCPP nanoparticles (NPs) with a D-π-A-π-D configuration. By utilizing the thermally activated delayed fluorescence (TADF) property, we achieved enhanced electrochemiluminescence (ECL) emission through complete exciton harvesting for radiative decay.

A portable CRISPR-Cas12a triggered photothermal biosensor for sensitive and visual detection of Staphylococcus aureus and Listeria monocytogenes.

The detection of foodborne pathogens is crucial for ensuring the maintenance of food safety. In the present study, a portable CRISPR-Cas12a triggered photothermal biosensor integrating branch hybrid chain reaction (bHCR) and DNA metallization strategy for sensitive and visual detection of foodborne pathogens was proposed. The sheared probes were utilized to block the locker probes, which enabled preventing the assembly of bHCR in the absence of target bacteria, while target bacteria can activate the cleavage of sheared probes through CRISPR-Cas12a.

The upregulation of Annexin A2 by TLR4 pathway facilitates lipid accumulation and liver injury via blocking AMPK/mTOR-mediated autophagy flux during the development of non-alcoholic fatty liver disease.

Background And Aims: Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease worldwide. In this study, we aimed to investigate the role and regulatory mechanism of Annexin A2 (ANXA2) in the pathogenesis of NAFLD.

Methods: Histological analyses and ELISA were used to illuminate the expression of ANXA2 in NAFLD and healthy subjects.

The expression mechanism of programmed cell death 1 ligand 1 and its role in immunomodulatory ability of mesenchymal stem cells.

Programmed cell death 1 ligand 1 (PD-L1) is an important immunosuppressive molecule, which inhibits the function of T cells and other immune cells by binding to the receptor programmed cell death-1. The PD-L1 expression disorder plays an important role in the occurrence, development, and treatment of sepsis or other inflammatory diseases, and has become an important target for the treatment of these diseases. Mesenchymal stem cells (MSCs) are a kind of pluripotent stem cells with multiple differentiation potential.