Heteroassembled gold nanoparticles with sandwich-immunoassay LSPR chip format for rapid and sensitive detection of hepatitis B virus surface antigen (HBsAg).

This study aimed to develop a more sensitive method for the detection of hepatitis B surface antigen (HBsAg) using heteroassembled gold nanoparticles (AuNPs). A single layered localized surface plasmon resonance (LSPR) chip format was developed with antigen-antibody reaction-based detection symmetry using AuNPs, which detected HBsAg at 10 pg/mL. To further improve the detection limit, a modified detection format was fabricated by fixing a secondary antibody (to form a heteroassembled sandwich format) to the AuNP monolayer, which enhanced the detection sensitivity by about 100 times.

Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meat.

A non-labeled, portable plasmonic biosensor-based device was developed to enable the ultra-sensitive and selective detection of Salmonella typhimurium in pork meat samples. Specifically, a plasmonic sensor, using the self-assembly of gold nanoparticles (AuNPs) to achieve a regulated diameter of 20 nm for the AuNP monolayers, was used to conduct high-density deposition on a transparent substrate, which produced longitudinal wavelength extinction shifts via a localized surface plasmon resonance (LSPR) signal. The developed aptamers conjugated to the LSPR sensing chips revealed an ultra-sensitive upper limit of detection (LOD) of approximately 10 cfu/mL for S.

Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen.

In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342-137.

Elastic limits in torsion of reciprocating nickel-titanium instruments.

Introduction: Reciprocating angle and torsional load at the superelastic limit were investigated for nickel-titanium (NiTi) instruments used with reciprocating movements.

Methods: Two reciprocating NiTi instruments (Reciproc R25 [VDW, Munich, Germany] and WaveOne Primary [Dentsply Maillefer, Ballaigues, Switzerland]) and ProTaper F2 (Dentsply Maillefer) were tested for 2 torsional conditions using a custom-designed testing device. Rotational angles were applied, and generated torque values were recorded.

Safety of the factory preset rotation angle of reciprocating instruments.

Introduction: This study aimed to investigate the torsional resistance of 2 reciprocating nickel-titanium instruments (Reciproc [VDW, Munich, Germany] and WaveOne [Dentsply Maillefer, Ballaigues, Switzerland]) operated at the maximum rotating angle in a proprietary motor.

Methods: With the file tip secured at various levels (3, 4, or 5 mm) of Reciproc R25 and WaveOne Primary, the distortion angles and torsional loads were monitored during counterclockwise movement at 2 rpm until fracture (n = 10 at each level) for a load-distortion graph. The rotation angles and loads at the beginning point of the plateau, the ultimate torsional strength, final fracture angle, and toughness were determined.