Interferon lambda 4 is a gut antimicrobial protein.

To withstand complex microbial challenges, the mammalian gut largely depends on the secretion of diverse antimicrobial proteins. Type III interferons (IFNλs) are ordinarily considered inducible antiviral cytokines involved in intestinal immunity. Unlike other IFNλs, we found that newly identified IFNλ4 is an intestinal antibacterial protein.

Multiple effects of carbon, sulfur and iron on microbial mercury methylation in black-odorous sediments.

Black-odorous sediments provide ideal conditions for microbial mercury methylation. However, the multiple effects of carbon, sulfur, and iron on the microbial methylmercury of mercury in black-odorous sediments remains unclear. In this study, we conducted mercury methylation experiments using sediments collected from organically contaminated water bodies, as well as black-odorous sediments simulated in the laboratory.

Mechanical regulation of lipid and sugar absorption by Piezo1 in enterocytes.

Obesity is primarily caused by excessive intake as well as absorption of sugar and lipid. Postprandial surge in distention pressure and intestinal motility accelerates the absorption of nutrients. The response of intestinal epithelial cells to mechanical stimulation is not fully understood.

Exogenous paths regulate electron transfer enhancing sediment phosphorus immobilization.

The lack of electron acceptors in anaerobic sediments leads to endogenous phosphorus release and low removal efficiency of organic pollutants. This study introduced electrodes and iron oxides into sediments to construct electron network transport chains to supplement electron acceptors. The sediment total organic carbon (TOC) removal efficiencies of closed-circuit (CC) and closed-circuit with Fe addition (CC-Fe) were estimated to be 1.

Identification of chronic non-atrophic gastritis and intestinal metaplasia stages in the Correa's cascade through machine learning analyses of SERS spectral signature of non-invasively-collected human gastric fluid samples.

The progression of gastric cancer involves a complex multi-stage process, with gastroscopy and biopsy being the standard procedures for diagnosing gastric diseases. This study introduces an innovative non-invasive approach to differentiate gastric disease stage using gastric fluid samples through machine-learning-assisted surface-enhanced Raman spectroscopy (SERS). This method effectively identifies different stages of gastric lesions.

Discovery of novel diaryl substituted isoquinolin-1(2H)-one derivatives as hypoxia-inducible factor-1 signaling inhibitors for the treatment of rheumatoid arthritis.

Since synovial hypoxic microenvironment significantly promotes the pathological progress of rheumatoid arthritis (RA), hypoxia-inducible factor 1 (HIF-1) has been emerged as a promising target for the development of novel therapeutic agents for RA treatment. In this study, we designed and synthesized a series of diaryl substituted isoquinolin-1(2H)-one derivatives as HIF-1 signaling inhibitors using scaffold-hopping strategy. By modifying the substituents on N-atom and 6-position of isoquinolin-1-one, we discovered compound 17q with the most potent activities against HIF-1 (IC = 0.

Gestational supplementation of Bifidobacterium, Lactobacillus, and Streptococcus thermophilus attenuates hepatic steatosis in offspring mice through promoting fatty acid β-oxidation.

Currently, Bifidobacterium, Lactobacillus, and Streptococcus thermophilus (BLS) are widely recognized as the crucially beneficial bacteria in the gut. Many preclinical and clinical studies have shown their protective effects against non-alcoholic fatty liver disease (NAFLD). However, whether gestational BLS supplementation could alleviate NAFLD in the offspring is still unknown.

Lipid accumulation product is a valid predictor of hepatic steatosis and nonalcoholic fatty liver disease.

To evaluate and compare lipid accumulation product (LAP) with alanine aminotransferase (ALT), aspartate aminotransferase (AST), visceral adiposity index (VAI) and triglyceride-glucose index (TyG) as biomarkers for hepatic steatosis and nonalcoholic fatty liver disease (NAFLD). LAP, ALT, AST, VAI and TyG were measured in 52 biopsy-proven NAFLD patients and 21 control subjects. Additionally, LAP was also measured in 448 ultrasound-proven NAFLD patients and 1009 control subjects.

TaiChiNet: Negative-Positive Cross-Attention Network for Breast Lesion Segmentation in Ultrasound Images.

Breast lesion segmentation in ultrasound images is essential for computer-aided breast-cancer diagnosis. To improve the segmentation performance, most approaches design sophisticated deep-learning models by mining the patterns of foreground lesions and normal backgrounds simultaneously or by unilaterally enhancing foreground lesions via various focal losses. However, the potential of normal backgrounds is underutilized, which could reduce false positives by compacting the feature representation of all normal backgrounds.

Elimination of methylmercury production potential in excessive sludge in wastewater treatment plants by sulfur addition.

Mercury ions (Hg(II)) in wastewater can accumulate and transform into the highly neurotoxic methylmercury (MeHg) in activated sludge. The release of MeHg can have severe environmental consequences, making the treatment of MeHg-contaminated sludge a pressing concern. In this study, we found that all the collected activated sludge samples, from different wastewater treatment plants in four cities, had the potential for Hg methylation.

Detection of Helicobacter pylori Infection in Human Gastric Fluid Through Surface-Enhanced Raman Spectroscopy Coupled With Machine Learning Algorithms.

Diagnostic methods for Helicobacter pylori infection include, but are not limited to, urea breath test, serum antibody test, fecal antigen test, and rapid urease test. However, these methods suffer drawbacks such as low accuracy, high false-positive rate, complex operations, invasiveness, etc. Therefore, there is a need to develop simple, rapid, and noninvasive detection methods for H.

Deterministic full-scenario analysis for maximum credible earthquake hazards.

Great earthquakes are one of the major threats to modern society due to their great destructive power and unpredictability. The maximum credible earthquake (MCE) for a specific fault, i.e.

Pyrolyzed sediment accelerates electron transfer and regulates rhodamine B biodegradation.

Electron transfer efficiency is a key factor that determined the removal of environmental pollution through biodegradation. Electron shuttles exogenously addition is one of the measures to improve the electron transfer efficiency. In this study, the sediment was pyrolyzed at different temperature to investigate its properties of mediating electron transfer and removing of rhodamine B (RhB) in microbial electrochemical systems (MESs).

GREnet: Gradually REcurrent Network With Curriculum Learning for 2-D Medical Image Segmentation.

Medical image segmentation is a vital stage in medical image analysis. Numerous deep-learning methods are booming to improve the performance of 2-D medical image segmentation, owing to the fast growth of the convolutional neural network. Generally, the manually defined ground truth is utilized directly to supervise models in the training phase.

Applying fulvic acid for sediment metals remediation: Mechanism, factors, and prospect.

Fulvic acid (FA) has been shown to play a decisive role in controlling the environmental geochemical behavior of metals. As a green and natural microbial metabolite, FA is widely used in environmental remediation because of its good adsorption complexation and redox ability. This paper introduces the reaction mechanism and properties of FA with metals, and reviews the progress of research on the remediation of metal pollutant by FA through physicochemical remediation and bioremediation.

Cross-kingdom synthetic microbiota supports tomato suppression of Fusarium wilt disease.

The role of rhizosphere microbiota in the resistance of tomato plant against soil-borne Fusarium wilt disease (FWD) remains unclear. Here, we showed that the FWD incidence was significantly negatively correlated with the diversity of both rhizosphere bacterial and fungal communities. Using the microbiological culturomic approach, we selected 205 unique strains to construct different synthetic communities (SynComs), which were inoculated into germ-free tomato seedlings, and their roles in suppressing FWD were monitored using omics approach.

Spatial distribution, environmental risks, and sources of potentially toxic elements in soils from a typical abandoned antimony smelting site.

The rapid development of the smelting industry increases the release of antimony (Sb) into the soil environment, which threatens human health and ecosystems. A total of 87 samples were collected from an abandoned Sb smelting site to evaluate pollution characteristics and environmental risks of the potentially toxic elements (PTEs). The contents of As, Cu, Ni, Pb, Sb, and Zn in the fresh soils determined by P-XRF were 131, 120, 60, 145, 240, and 154 mg/kg, respectively, whilst following drying, grinding, and sieving pretreatments, the corresponding contents increased to 367, 179, 145, 295, 479, and 276 mg/kg, respectively.

High-efficiency genome editing of an extreme thermophile using endogenous type I and type III CRISPR-Cas systems.

is an attractive species in the bioindustry due to its valuable natural products, abundant thermophilic enzymes, and promising fermentation capacities. However, efficient and versatile genome editing tools are not available for this species. In this study, we developed an efficient genome editing tool for HB27 based on its endogenous type I-B, I-C, and III-A/B CRISPR-Cas systems.

Treatment and remediation of metal-contaminated water and groundwater in mining areas by biological sulfidogenic processes: A review.

Heavy metal pollution in the mining areas leads to serious environmental problems. The biological sulfidogenic process (BSP) mediated by sulfidogenic bacteria has been considered an attractive technology for the treatment and remediation of metal-contaminated water and groundwater. Notwithstanding, BSP driven by different sulfidogenic bacteria could affect the efficiency and cost-effectiveness of the treatment performance in practical applications, such as the microbial intolerance of pH and metal ions, the formation of toxic byproducts, and the consumption of organic electron donors.

A comprehensive review on the photocatalytic inactivation of Microcystis aeruginosa: Performance, development, and mechanisms.

Harmful algae blooms (HABs), caused by severe eutrophication and extreme weather, have spread all over the world, posing adverse effects on eco-environment and human health. Microcystis aeruginosa is the dominant harmful cyanobacterial species when HABs occur, and the toxic metabolites produced by it, microcystins, are even fatal to humans. Photocatalytic technology has received wide attention from researchers for its clean and energy-efficient features, while the basic mechanisms and modification methods of photocatalysts have also been widely reported.

Mesoscale Modeling Study on Mechanical Deterioration of Alkali-Aggregate Reaction-Affected Concrete.

The alkali-aggregate reaction (AAR) is a harmful chemical reaction that reduces the mechanical properties and weakens the durability of concrete. Different types of activated aggregates may result in various AAR modes, which affect the mechanical deterioration of concrete. In this paper, the aggregate expansion model and the gel pocket model are considered to represent the two well-recognized AAR modes.

Pollution simulation and remediation strategy of a zinc smelting site based on multi-source information.

Contaminated sites pose a significant risk to human health and the regional environment. A comprehensive study was dedicated to improving the understanding of the contamination condition of a smelting site by integrating multi-source information through 3D visualization techniques. The results showed that 3D visualization reveals excellent potential for application in the environmental studies to finely depict contamination in soils and establish relationships with geological features, hydrological conditions, and sources of contamination.

Role of sulfur biogeochemical cycle in mercury methylation in estuarine sediments: A review.

Estuaries are sinks for mercury, in which the most toxic mercury form, neurotoxic methylmercury (MeHg), is produced by mercury methylators and accumulates in estuarine sediments. In the same area, the microbial sulfur cycle is triggered by sulfate-reducing bacteria (SRB), which is considered as the main mercury methylator. In this review, we analyzed the sulfur and mercury speciation in sediments from 70 estuaries globally.