[Establishment of a Multidrug Resistance Cell Line A549/cDDP of Human Lung Adenocarcinoma and Expression Analysis of Multidrug Resistance-Associated Genes.].

Background: It has been proven that chemotherapy failure caused by multidrug resistance in lung tumor cells is the main cause for the patient's survival rate. The aim of this study is to establish a multidrug resistance cell line of human lung adenocarcinoma and study the mechanism of multidrug resistance.

Methods: Human lung adenocarcinoma cell line A549 was induced to multidrug resistance cell line A549/cDDP by intermittent administration of high dose of cisplatin (cDDP).

[Incoherent broadband cavity enhanced absorption spectroscopy based on LED].

High sensitivity incoherent broadband cavity enhanced absorption spectroscopy based on high power LED was developed. The detection sensitivity was demonstrated by measuring the absorption of NO2 over the wavelength range of 472. 3-479.

Heme oxygenase-1 expression in rats with acute lung rejection and implication.

This study investigated the expression of hemeoxygenase-1 (HO-1) in rats with acute lung rejection and its implication. A valid rat orthotopic left lung transplantation model (SD rat-->Wistar rat) was established by using an improved three-cuff anastomosis technique. The rats were divided into control group, CoPP (HO-1 inducer)-treated group and ZnPP (HO-1 inhibitor)- treated group.

Pediatric mandibular reconstruction after benign tumor ablation using a vascularized fibular flap.

This study evaluated the aesthetic and functional outcomes of vascularized fibular flaps used for pediatric mandibular reconstruction. Six pediatric patients ranging in age from 12 to 15 years underwent mandibular reconstruction using a vascularized fibula flap with a skin paddle, after benign tumor ablation. All of the flap transfers were successful, and there were no complications.

Liquid nitrogen cryotherapy of lip mucosa hemangiomas under inhalation general anesthesia with sevoflurane in early infancy.

Mucous membrane hemangiomas of the lip are common benign vascular tumors of infancy. This clinical study evaluated the efficacy and safety of liquid nitrogen cryotherapy of mucous membrane hemangiomas of the lip in early infancy. It was a retrospective review of 127 pediatric patients with hemangiomas involving the lips who underwent liquid nitrogen cryotherapy under inhalation general anesthesia with sevoflurane.

Fast track clinical pathway implications in esophagogastrectomy.

Aim: To investigate the feasibility of fast track clinical pathway for esophageal tumor resections.

Methods: One hundred and fourteen patients with esophageal carcinoma who underwent esophagogastrectomy from January 2006 to October 2007 in our department were studied. Fast track clinical pathway included analgesia control, fluid infusion volume control, early ambulation and enteral nutrition.

Detection of viral aerosols by use of real-time quantitative PCR.

PCR quantification is regarded as one of the most promising techniques for real-time identification of bio-aerosols. We have, therefore, validated a QPCR assay for quantification of a viral aerosol sample using the double-stranded DNA-binding dye SYBR green I, an economical alternative for quantification of target microorganisms. To achieve this objective we used mycobacteriophage D29 as model organism.

[The influence of CO2 molecular relaxation dynamics on photoacoustic signal in near infrared (NIR) range].

Photoacoustic spectroscopy (PAS) based on tunable diode laser (TDLPAS) is a widely recognised method for its excellent performance in trace gas monitoring and spectral analysis. This spectral technique provides many advantages such as high selectivity and sensitivity, on-line, real time and contactless measurements in a compact experimental arrangement. In the present paper, a resonant PA spectrometer based on a low-power near infrared distributed feedback (DFB) diode laser and a home-made resonant PA cell operating on its first longitudinal mode is developed, and the PA system has the advantage of simple experimental setup, easy operation, and low-cost, room temperature operating, etc.

The trapezius osteomyocutaneous island flap for reconstructing hemimandibular and oral defects following the ablation of advanced oral malignant tumours.

Objective: This study presents an effective repair method for the hemimandibular and oral defects produced during the ablation of advanced oral malignant tumours.

Methods: Nine patients (five males and four females ranging in age from 18 to 74 years; mean age 51.3 years) with advanced oral malignant tumours were treated at our institution.

[An epidemiological survey on saturnism among children due to lead pollution released from township enterprise].

Objective: To understand the current situations of saturnism and blood lead levels of children resided in village and circumjacent areas, and to know its relations with sex, age and other factors on children' s health as to providing some evidences for prevention and control.

Methods: An epidemiological survey was conducted for finding out the pollution sources and for a better understanding of the surrounding environment. All 221 children under 14 years old, from the lead pollution villages and surrounding establishments were enrolled, and their blood lead levels were detected by graphite atomizer absorption spectrophotometer method.

[The role of autophagy in human lung cancer cell line A549 induced by Vinorelbine.].

Background: It has been proven that many kinds of drug could induce autophagy in tumor cells. The aim of the study is to verify whether Vinorelbine could induce autophagy in A549 cell lines, and to investigate the role in the effectiveness of death induced by Vinorelbine.

Methods: Cells survival rate was examined by tetrazolium bromide (MTT) colorimetry; interactive laser microscopy and flow cytometry were used to detect LC3 expression levels qualitatively and quantitatively, respectively; apoptosis rates were checked by flow cytometry; treatment of A549 cells with 3-MA and Vinorelbine, the apoptosis rates and cell death rates were examined by flow cytometry.

Nuclear transfer-mediated rescue of the nuclear genome of nonviable mouse cells frozen without cryoprotectant.

Nuclear transfer (NT) provides an opportunity for clonal amplification of a nuclear genome of interest. Here, we report NT-mediated reprogramming with frozen mouse cells that were nonviable because they were frozen at -80 degrees C for up to 342 days without a cryoprotectant. We derived eight embryonic stem (ES) cell lines from cloned blastocysts by conventional NT procedure and five ntES (nuclear transfer embryonic stem) cell lines by a modified NT procedure in which a whole cell instead of a nucleus was injected into an enucleated oocyte.

[Evaluation of the articulator function in the hemi-tongue defect patients after radical tongue cancer resection and simultaneous reconstruction with forearm flap or primary close].

Objective: To explore the effect of the patients' articulator function after reconstruction of hemi-tongue defect with forearm flap (FAP) or prime close (PC).

Methods: 36 patients who underwent hemiglossectomy were investigated after radical surgery for TSCC. 20 cases were reconstructed with FAP flaps and 16 with primary closure.

Helix-rich transient and equilibrium intermediates of equine beta-lactoglobulin in alkaline buffer.

Acidic buffer conditions are known to stabilize helix-rich states of even those proteins with a predominantly beta-sheet native secondary structure. Here we investigated whether such states also exist under alkaline buffer conditions. The guanidine hydrochloride (GuHCl)-induced unfolding transition and kinetic refolding of equine beta-lactoglobulin (ELG) by GuHCl-jump were investigated at pH 8.

[Reverse facial artery-submental artery island myocutaneous flap for reconstruction of oral and maxillofacial defects following cancer ablation].

Objective: To evaluate the reliability of the reverse facial artery-submental artery island myocutaneous flap for repairing the oral and maxillofacial defects.

Methods: Eighteen oral and maxillofacial defects were repaired with reverse facial artery-submental artery island myocutaneous flaps following resection of malignant tumors. The ages of the patients ranged from 28 to 90 years; 11 were male and 7 were female.

Gene expression profile of pulmonary tissues in different phases of lung ischemia-reperfusion injury in rats.

In order to provide us new clues to induce some endogenous protective molecular mechanisms, the changes in gene expression profile induced by ischemia-reperfusion in pulmonary tissues of rats were investigated and the dynamic mechanism of pulmonary ischemia-reperfusion injury was elucidated. Thirty male Wistar rats were randomly divided into 6 groups: 5 ischemia-reperfusion (I/R) groups (I/R 0-h, I/R 1-h, I/R 3-h, I/R 6-h, I/R 24-h) and control group (n=5 in each). An in situ ischemia-reperfusion lung injury rat model was established by occluded hilus of lung.

A stable alpha-helix-rich intermediate is formed by a single mutation of the beta-sheet protein, src SH3, at pH 3.

Recently, we have found a transient intermediate on the folding pathway of src SH3. Intending to investigate the structure of the transient intermediate, we tested a mutant of src SH3, named A45G, using circular dichroism, fluorescence and X-ray solution scattering, and incidentally found that it forms a stable alpha-helix-rich intermediate (I(eq)) (different from the native beta-sheet-based secondary structure) at pH 3.0, but contains only beta-sheets at pH 6.

[Lower trapezius myocutaneous flap for repairing defects in head and neck region].

Objective: To evaluate the anatomy basis and clinical effects of the lower trapezius myocutaneous flap to reconstruct defects in the head and neck.

Methods: From Jan. 2000 to Jan.

An alpha-helical burst in the src SH3 folding pathway.

Src SH3 is a small all-beta-sheet protein composed of a single domain. We studied the folding behavior of src SH3 at various conditions by circular dichroism (CD), fluorescence, and X-ray solution scattering methods. On the src SH3 folding pathway, an alpha-helix-rich intermediate appeared not only at subzero temperatures but also above 0 degrees C.

Mice cloned from skin cells.

Adult stem cells represent unique populations of undifferentiated cells with self-renewal capacity. In many tissues, stem cells divide less often than their progeny. It has been widely speculated, but largely untested, that their undifferentiated and quiescent state may make stem cells more efficient as donors for cloning by nuclear transfer (NT).

[Expression and significance of inducible nitric oxide synthase and vascular endothelial growth factor in carcinogenesis of hamster cheek pouch].

Background & Objective: Inducible nitric oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) are related to carcinogenesis and development of tumors. This study was to explore the correlations of the expression of iNOS and VEGF to the angiogenesis in carcinogenesis of Golden hamster cheek pouch.

Methods: A total of 50 Golden hamsters were divided into 2 groups: 40 in experiment group, 10 in control group.

Solvent-tuning the collapse and helix formation time scales of lambda(6-85)*.

The lambda(6-85)(*) pseudo-wild type of lambda repressor fragment is a fast two-state folder (k(f) approximately 35 microsec(-1) at 58 degrees C). Previously, highly stable lambda(6-85)(*) mutants with k(f) > 30 microsec(-1) have been engineered to fold nearly or fully downhill. Stabilization of the native state by solvent tuning might also tune lambda(6-85)(*) away from two-state folding.

[The analysis of complications after surgical treatment of primary brain stem diseases].

Objective: To analyze the complications of the surgical treatment on primary brain stem diseases.

Methods: From January 2000 to December 2005, 38 patients with primary brain stem diseases underwent surgical operation. The data of the patients were retrospectively analyzed.

[Combined repair of large defect caused by radical surgery of advanced tongue cancer with rib-major pectoralis myocutaneous flap carrying costal parietal pleura].

Objective: To explore the clinical value and safety of using rib-major pectoralis myocutaneous flap carrying costal parietal pleura in combined repair of large soft and hard tissue defect caused by radical surgery of advanced tongue cancer.

Methods: Six patients with advanced tongue carcinoma involving the floor of mouth and mandible were performed combined radical neck dissection with glossectomy and mandibulectomy, which caused large soft and hard tissue defect. Six rib-major pectoralis myocutaneous flaps carrying costal parietal pleura were transferred for immediate repair of the large defects.

Perivascular paclitaxel wraps block arteriovenous graft stenosis in a pig model.

Background: Haemodialysis vascular access dysfunction is currently a huge clinical problem. In an attempt to reduce the morbidity associated with haemodialysis vascular access dysfunction, we have previously developed and validated a local perivascular paclitaxel release system that has been shown to release paclitaxel for at least 3 weeks. The aim of the current study was to evaluate the in vivo use of these perivascular wraps (for both safety and efficacy) at different time points in our pig model of arteriovenous graft stenosis.