Publications by authors named "Jinshan Cao"

(AMK) as an oriental medicine has been used in the treatment of threatened abortion. (AT-I) is one of the major bioactive components of AMK. This study aimed to investigate the effect of AT-I on the secretion of estradiol (E) and progesterone (P) in feline ovarian granulosa cells (FOGCs), which is necessary for pregnancy.

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It is proven through transmission electron microscope (TEM) analysis that solar sensitizer CuSnS (CTS) dots prepared via the hot-injection route are nonspherical, polyhedral nanocrystals with the size of ∼11 nm. CTS dots were deposited into a porous TiO layer to form CTS/TiO, an effective type II heterojunction in photoanodes. The electronic and energy band structures of TiO and CTS were studied by the plane-wave ultrasoft pseudopotential method based on density functional theory (DFT) and verified by ultraviolet-visible (UV-vis) spectroscopy.

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  • Researchers developed a new drug delivery system using hen egg low-density lipoprotein (heLDL) to transport the antibiotic ceftiofur (CEF), creating a formulation called CEF-heLDL that has a stable structure and efficient drug loading.
  • In lab tests, CEF-heLDL effectively entered cells and targeted lysosomes, showing better antibacterial activity, especially against Staphylococcus aureus, compared to the CEF-alone group.
  • The study found that high doses of CEF-heLDL were safe for mice, suggesting that heLDL can be a cost-effective and safe carrier for delivering other antibiotics across cell membranes.
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Anal swabs of 1-month-old Holstein calves with diarrhea were collected from an intensive cattle farm, and a highly pathogenic Escherichia coli strain was obtained by isolation and purification. To study the virulence and resistance genes of pathogenic E. coli that cause diarrhea in calves, a strain of E.

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Different antibiotics are used to treat mastitis in dairy cows that is caused by Antimicrobial resistance in food-producing animals in China has been monitored since 2000. Surveillance data have shown that the prevalence of multiresistant in animals has increased significantly. This study aimed to investigate the occurrence and molecular characteristics of resistance determinants in strains ( = 105) obtained from lactating cows with clinical bovine mastitis (CBM) in China.

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Staphylococcus aureus (S. aureus) is one of the most infamous and widespread bacterial pathogens, causing a hard-to-estimate number of uncomplicated skin infections and probably hundreds of thousands to millions of more severe, invasive infections globally per year. S.

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S. aureus isolated from bacterial bovine endometritis is common in epidemiological reports, but is often ignored as a subclinical pathogenic microorganism. In a previous study, we showed that live S.

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Prostaglandin D (PGD) synthesis is closely associated with the innate immune response mediated by pattern recognition receptors (PPRs). We determined PGD synthesis whether mediated by Toll-like receptor 2 (TLR2), TLR4 and Nod-like receptor pyrin domain-containing protein 3 (NLRP3) in Escherichia coli (E. coli)-, lipopolysaccharide (LPS)- and Braun lipoprotein (BLP)-stimulated macrophages.

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Article Synopsis
  • - The study investigates the role of the NLRP3 inflammasome in the inflammatory response to E. coli infections, focusing on its effects in comparison to Toll-like Receptors 2 and 4 (TLR2 and TLR4).
  • - Results show that mice lacking TLR2, TLR4, or NLRP3 had less liver damage and lower levels of inflammatory markers (TNF-α and IL-1β) after exposure to E. coli or its components than wild-type mice.
  • - The findings suggest that NLRP3 is significant in the body’s defense against E. coli infections, alongside TLR2 and TLR4, highlighting it as a potential target for future therapies
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Introduction: In clinical settings, dairy cows are often attacked by pathogenic bacteria after delivery, especially (). Neutrophils have long been regarded as essential for host defense against . Prostaglandin E (PGE) can additionally be used as an inflammatory mediator in pathological conditions to promote the repair of inflammatory injuries.

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  • E. coli, a Gram-negative bacterium, can cause serious diseases in mammals, and its outer membrane components, like Braun lipoprotein (BLP), play a role in triggering the immune response.
  • The study used mouse models to explore how BLP affects inflammation and survival during E. coli infections, revealing that BLP presence reduces organ damage and levels of harmful cytokines while boosting anti-inflammatory responses.
  • BLP modulates inflammatory signals through specific pathways (MAPK and NF-κB) in macrophages, indicating its protective role in the host's response to E. coli infections.
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  • The unregulated use of antimicrobial drugs on dairy farms is causing increased drug resistance in pathogens, particularly Escherichia coli, leading to serious health issues in calves.
  • The study conducted in Hohhot analyzed cases of calf diarrhea, isolating 21 strains of E. coli, with over 50% showing resistance to multiple antibiotics, including penicillin and ampicillin.
  • Results revealed significant presence of virulence genes, indicating pathogenic potential, with various antibiotic resistance genes detected, highlighting the urgency for better management practices in dairy farming.
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Dairy cows often develop different degrees of endometritis after calving and this is attributed to pathogenic bacterial infections such as by Escherichia coli and Staphylococcus aureus. Infection of the bovine endometrium causes tissue damage and increases the expression of prostaglandin D (PGD), which exerts anti-inflammatory effects on lung inflammation. However, the roles of PGD and its DP1 receptor in endometritis in cows remain unclear.

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When some sub-images lack ground control points (GCPs) or GCPs are not evenly distributed, the estimated camera parameters are often deviated in in-orbit geometric calibration. In this study, a feasible in-orbit geometric calibration method for multi-linear array optical remote sensing satellites with tie constraints is presented. In the presented method, both GCPs and tie points are employed.

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Prostaglandin E (PGE ) is able to induce the expression of several growth factors and enzymes in cattle endometria. However, the specific type of PGE receptors which mediates this effect is not fully clear. In this study, the role of prostaglandin E receptor 2 (PTGER2) in PGE -mediated induction of growth factors and enzymes expression in cattle endometrial explants and epithelial cells were investigated.

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Acute lung injury (ALI) is an inflammatory lung disease that is caused by bacterial infection. Lipopolysaccharide (LPS), a prototype pathogen-associated molecular pattern (PAMP) from Gram-negative bacteria such as (), is an essential risk factor for ALI. LPS and induced the activation of mitogen-activated protein kinase (MAPK) and nuclear factor kappaB (NF-κB) signaling pathways, which led to the increasing immune molecule transcription, including pro-inflammatory cytokine and chemokine secretion.

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Article Synopsis
  • Staphylococcus aureus (S. aureus) is a germ that can make mammals sick and its effects are linked to certain immune receptors in the body.
  • Researchers found that when mice lacked these receptors (TLR2, TLR4, and NLRP3), they got sicker from S. aureus infections.
  • The role of these receptors changes over time; in the early stages of infection (3-6 hours), they help fight the infection, but after 24 hours, they seem to make things worse by increasing harmful substances in the body.
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It is known that prostaglandin E (PGE) induces proliferation of epithelia in bovine endometrial explants, however, the detailed mechanism of regulation of PGE in inducing bovine endometrial epithelial cell (bEEC) proliferation is unclear. In this study, we determined whether proliferation of bEECs is promoted by PGE-prostaglandin E receptor 2 (PTGER2) signaling activation through cell cycle regulation. The results demonstrated that bEECs proliferation was induced by treatment of PGE and PTGER2 agonist butaprost.

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  • - Staphylococcus aureus (S. aureus) is a key player in bovine mastitis but exhibits weak pro-inflammatory responses in mammary glands; this study investigates its role in bovine endometritis and inflammation.
  • - In vitro experiments show that heat-killed S. aureus (HK-SA) activates higher levels of several pro-inflammatory factors compared to live S. aureus (LSA), which produces increased expression of different inflammatory markers and signaling pathways in bovine endometrial tissue.
  • - The findings suggest S. aureus activates distinct signaling pathways for inflammation, with HK-SA enhancing cytokine secretion and LSA primarily promoting PGE accumulation, marking the first distinction in responses to these different forms of S
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This article is aimed at analyzing the structure and function of the spike (S) proteins of porcine enteric coronaviruses, including transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) by applying bioinformatics methods. The physical and chemical properties, hydrophilicity and hydrophobicity, transmembrane region, signal peptide, phosphorylation and glycosylation sites, epitope, functional domains, and motifs of S proteins of porcine enteric coronaviruses were predicted and analyzed through online software. The results showed that S proteins of TGEV, PEDV, SADS-CoV, and PDCoV all contained transmembrane regions and signal peptide.

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The HaiYang-1C coastal zone imager (CZI) consists of two independent cameras with a total image swath of approximately 1000 km. In order to obtain precise imaging parameters of the CZI cameras, a feasible in-orbit geometric calibration approach with multiple fields is presented. First, the master CCD is calibrated with a calibration field.

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Prostaglandin E2 (PGE) enhances Staphylococcus aureus infection but its mechanism is not well understood. Here, we examined the effect of PGE on Staphylococcal Protein A (SPA) expression in bovine endometrium and determined the role of select PGE receptors (i.e.

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Bovine endometrium infection with gram-negative bacteria commonly causes uterine diseases. Previous studies indicate that prostaglandin E (PGE) is an inflammatory mediator in bacterial endometritis. However, the mechanism underlying lipopolysaccharide (LPS)-induced inflammatory response regulation in bovine endometrial explants remains elusive.

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Bovine endometritis is one of the major postpartum diseases associated with infertility and subfertility, decreasing the benefit of dairy industry. It is important to develop alternate therapies for endometritis in the context of drug residues in the milk and hormone disorder in the estrous cycle. α7 nicotine acetylcholine receptor has been identified as the core of 'cholinergic anti-inflammatory pathway (CAP)', which is a potential drug target to inflammatory diseases.

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