Publications by authors named "Jinquan Fan"

Article Synopsis
  • The study focused on how tilapia brain cells respond genetically to hypertonic stress, identifying a total of 331 known and 163 novel miRNAs involved in this response.
  • It found that 16 miRNAs were significantly upregulated and 11 downregulated, affecting key processes like metabolism, immune response, and neural regulation, with implications for neurotrophic and synaptic signaling pathways.
  • The research highlights the importance of miRNA expression changes in regulating osmotic pressure, providing a foundation for further study on miRNA functions and potentially aiding aquaculture practices in harsh water conditions.
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Background: Saline-alkaline water aquaculture has become a key way to mitigate the reduction of freshwater aquaculture space and meet the increasing global demand for aquatic products. To enhance the comprehensive utilization capability of saline-alkaline water, it is necessary to understand the regulatory mechanisms of aquatic animals coping with saline-alkaline water. In this study, our objective was to elucidate the function of proline metabolism in the alkaline adaptation of Nile tilapia (Oreochromis niloticus).

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Peritrophic membrane (PM) is a pellicle structure present in the midgut of some invertebrates, such as insects and crustaceans. It could isolate harmful components and pathogens in food from intestinal epithelial cells; and it also plays a role in improving digestion and absorption efficiency. So PM is important for survival of its owner.

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  • The study investigates the role of STING (Stimulator of Interferon Genes) in the immune response of largemouth bass (Micropterus salmoides) to largemouth bass virus (LMBV), finding that MsSTING and related genes are up-regulated following infection.
  • A small peptide known as Fusion peptide (FP) was found to interact with MsSTING and impacted gene expression, leading to significant changes in 706 genes after treatment.
  • KEGG analysis indicated these gene changes were related to immune response and signaling pathways, and the survival rate of bass infected with LMBV significantly decreased with FP treatment, highlighting MsSTING's critical role in combating LMBV infection.
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RNA interference (RNAi) is a conservative and important functional pathway in eukaryocyte. It regulates the expression of genes that are engaged in a variety of cellular physiological functions. Among the functions of RNAi, its antiviral function have attracted many attentions.

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  • The study investigates the role of LvSmad5, a protein in the shrimp species Litopenaeus vannamei, in responding to environmental stress, particularly focusing on its location in the cytoplasm and nucleus under stress conditions.
  • Through experiments in Drosophila S2 cells, the researchers found that different forms of LvSmad5 led to significant changes in gene expression, particularly in pathways related to endoplasmic reticulum (ER) stress.
  • Further investigation into the gene LvTRC8, related to ER-stress response, suggests that nuclear LvSmad5 plays a crucial role in the shrimp's ability to cope with environmental stressors.
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Article Synopsis
  • - The study investigates the impact of endoplasmic reticulum (ER) stress on the health of the whiteleg shrimp (Litopenaeus vannamei), highlighting its role in aquatic animal diseases and the need for a better understanding of environmental stress responses.
  • - Using RNA-Seq technology, researchers found significant changes in gene expression, with 286 genes upregulated and 473 downregulated when shrimp were treated with Thapsigargin, indicating the activation of the ER-stress response.
  • - The findings suggest that ER-stress affects immune-related genes, particularly Prophenoloxidase activating enzyme 2 (LvPPAE2), which plays a role in shrimp's defense against pathogens, providing insights into how shrimp
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In shrimp, several glutathione peroxidase (GPX) genes have been cloned and functionally studied. Increasing evidence suggests the genes' involvement in white spot syndrome virus (WSSV)- or -infection resistance. In the present study, a novel GXP gene () was cloned in .

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The interplay between virus and host has been one of the hot spot in virology, and it is also the important aspect of revealing the mechanism of virus infection. Increasing studies revealed that several key molecules took part in the process of virus-host interaction. White spot syndrome virus (WSSV) has been proved to affect several physiological processes of the host cells, especially apoptosis.

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Seeking out fish meal (FM) alternatives is an important requirement for aquaculture all over the world. And most practitioners believe that the plant protein is most potential for FM surrenal. While high plant protein feed caused some common problems in aquatic livestock: the absorption rate and growth rate are decreased, and even caused digestive tract inflammation.

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Cell survival is based on the stability of intracellular state. It was well known that biochemical reactions in cells require specific intracellular environments, such as pH and calcium concentration. While the mechanism of stabilizing the intracellular environment is complex and far from clear.

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C-type lectins (CTLs), which bind carbohydrates in a Ca-dependent manner, are involved in many cellular activities, especially immunity. CTLs play important roles in both the antibacterial and the antiviral immune response and are also associated with autoimmunity. Several CTLs have been investigated in crustaceans, primarily with respect to their function in the immune response.

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