Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
November 2013
Objective: To study the dynamic change of proliferation, subsets and apoptosis of splenocytes of BALB/c mice immunized with recombinant Bb(pGEX-Sj26GST-Sj32) vaccine of Schistosoma japonicum.
Methods: A total of 96 BALB/c mice were randomly divided into 2 groups (n=48 per group) and immunized with the recombinant vaccine orally (PO group) and intranasally (IN group) respectively. Then autopsies were made in 4 mice of each group 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 weeks after immunization to separate splenocytes.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
July 2013
Objective: To investigate the dynamic changes of the immune responses in mice immunized with a recombinant (Bifidobacterium bifidum, Bb) (pGEX-Sj14-3-3) vaccine of Schistosoma japonicum.
Methods: BALB/c mice were immunized orally or intranasally by the vaccine. At the interval of every two weeks up to the 22nd week of post-vaccination, the levels of serum IgG, IgG subclass, IgE and IgA were determined by ELISA.