Construction of a biocompatible decellularized porcine hepatic lobe for liver bioengineering.

Objective: One of the major obstacles in applying decellularized organs for clinical use is the recellularization step, during which huge numbers of cells are required to develop whole livers. We established a simple protocol for constructing a bioartificial hepatic lobe and investigated its biocompatibility.

Methods: The right lateral lobe of porcine liver was decellularized using 0.

Three dimensional culture of HepG2 liver cells on a rat decellularized liver matrix for pharmacological studies.

Three-dimensional in vitro tumor models are needed to obtain more information about drug behavior in tumors. The aim of this study is to establish a new model for hepatocellular carcinoma (HCC) using decellularized rat livers. After generating the rat liver scaffolds, HepG2 liver cancer cells were perfused via the portal vein and placed in a bioreactor for 10 days.