16S rDNA sequencing reveal synergistic effects of silkworm feces and earthworms on nutrient-poor soil microbial community structure and function in Guangxi.

This study evaluates the synergistic effects of silkworm feces and earthworms on nutrient-poor acidic red soils in Guangxi, China. Using 16S rDNA amplicon sequencing, soil samples from untreated fields, silkworm feces, earthworm gut contents, and soils treated with silkworm feces combined with three earthworm densities (50, 80, and 110 worms/kg) were compared. The earthworm gut microbiome increased in diversity in a density-dependent manner, while treated soils displayed enhanced microbial richness-with the 80 worms/kg treatment showing the highest diversity (605 genera).

Highly stretchable, self-healing, adhesive, 3D-printable and antibacterial double-network hydrogels for multifunctional wearable sensors.

Conductive hydrogels based on sodium alginate (SA) have potential applications in human activity monitoring and personal medical diagnosis due to their good conductivity and flexibility. However, most sensing SA-hydrogels exhibit poor mechanical properties and lack of self-healing, self-adhesive, and antibacterial properties, greatly limiting their practical applications. Therefore, in this paper, a multifunctional double-network PAA-SA hydrogel consisting of poly(acrylic acid) (PAA) and sodium alginate (SA) was prepared by a simple strategy.

High-performance conductive double-network hydrogel base on sodium carboxymethyl cellulose for multifunctional wearable sensors.

Sodium carboxymethyl cellulose showed great potential in wearable intelligent electronic devices due to its low price and good biocompatibility. This research aimed to develop a novel conductive hydrogel with stretchable, self-healing, self-adhesive, antibacterial, 3D printable properties, for the development of multifunctional flexible electronic materials based on sodium carboxymethyl cellulose. A multifunctional conductive hydrogel based on sodium carboxymethyl cellulose (SCMC) was synthesized by simple polymerization of SCMC, acrylic acid (AA) and alkaline calcium bentonite (AC-Bt).

Effects of PEF on Cell and Transcriptomic of .

Pulsed electric field (PEF) is an up-to-date non-thermal processing technology with a wide range of applications in the food industry. The inactivation effect of PEF on was different under different conditions. The inactivated number was 1.

miR-34c-5p inhibited fibroblast proliferation, differentiation and epithelial-mesenchymal transition in benign airway stenosis via MDMX/p53 pathway.

Benign airway stenosis (BAS) means airway stenosis or obstruction that results from a variety of non-malignant factors, including tuberculosis, trauma, benign tumors, etc. In consideration of the currently limited research on microRNAs in BAS, this study aimed to explore the role and mechanism of miR-34c-5p in BAS. The expression of miR-34c-5p in BAS granulation tissues showed a significant down-regulation compared with the normal control group.

Differential expression of miRNAs revealed by small RNA sequencing in traumatic tracheal stenosis.

Traumatic tracheal stenosis (TTS) is a major cause of complex difficult airways, without clinically definitive efficacious drugs available. The aim of this study was to provide a general view of interactions between micro and messenger ribonucleic acids (miRNAs and mRNAs) and many potential mechanisms in TTS small RNA sequencing. In this study, the identification of miRNAs was completed using small RNA sequencing and samples from four TTS patients and four normal control cases.

Targeted therapy for multiple gene mutations in multiple metastases of advanced gastric cancer: a case report.

In China, gastric cancer is the second most common cause of cancer-related death, after lung cancer. At present, the morbidity and mortality rates of gastric cancer are increasing, and targeted therapy for gastric cancer has become a research hotspot. Herein, we report a patient with multiple metastases from advanced gastric cancer.

Down-regulation of miR-21-5p by pirfenidone to inhibit fibroblast proliferation in the treatment of acquired tracheal stenosis.

Objective: Treatment options for acquired tracheal stenosis (ATS) are limited due to a series of pathophysiological changes including inflammation and cell proliferation. Micro ribonucleic acid-21-5p (miR-21-5p) may promote the excessive proliferation of fibroblasts. However, various types of fibrosis can be prevented with pirfenidone (PFD).

Identification and verification of microtubule associated genes in lung adenocarcinoma.

Associated with high morbidity and mortality, lung adenocarcinoma (LUAD) is lacking in effective prognostic prediction and treatment. As chemotherapy drugs commonly used in clinics, microtubule-targeting agents (MTAs) are limited by high toxicity and drug resistance. This research aimed to analyze the expression profile of microtubule-associated genes (MAGs) in LUAD and explore their therapy efficiency and impact on prognosis.

Distribution and chemotactic mechanism of CD4 T cells in traumatic tracheal stenosis.

A systemic and local inflammatory immune imbalance is thought to be the cause of traumatic tracheal stenosis (TS). However, with CD4 T lymphocytes being the predominant immune cells in TS, the mechanism of action and recruitment has not been described. In our research, using flow cytometry, ELISA, immunofluorescence, and Transwell chamber assays, the expression, distribution, and potential chemotactic function of CD4 T cells in TS patients were examined before and after treatment.

Analysis of clinical characteristics of 617 patients with benign airway stenosis.

Introduction: Benign airway stenosis (BAS), namely airway narrowing caused by a variety of benign lesions, can lead to varying degrees of breathing difficulties and even death due to asphyxia. This study aimed to elucidate the clinical characteristics of BAS, including etiology, treatment and pathology, by analyzing the clinical data of BAS patients.

Methods: A retrospective analysis was conducted using the clinical data of 617 BAS cases from January 2017 to December 2022.

Identification of potential prognostic markers for lung adenocarcinoma using comprehensive analysis.

Lung adenocarcinoma (LUAD) is a common malignancy throughout the world with high levels of mortality and morbidity. In the present study, potential biomarkers and treatment targets for LUAD were investigated using data from The Cancer Genome Atlas. Overall, 4,485 differentially expressed genes (DEGs) were identified (1,857 upregulated and 2,628 downregulated) between tumor and adjacent control tissues.

Clinical course and management of insidious adrenal crisis manifested initially as hyperpyrexia secondary to pembrolizumab: Case reports and literature review.

Immune checkpoint inhibitors (ICIs) are novel drugs with a dramatic survival benefit in patients with advanced malignancies. With the widespread use, several immune-related adverse events (irAEs) have emerged, which may be life-threatening. Herein we report two patients with adrenal crisis who received anti-programmed cell death protein 1 (PD-1) (pembrolizumab) therapy.

Prognostic Risk Signature and Comprehensive Analyses of Endoplasmic Reticulum Stress-Related Genes in Lung Adenocarcinoma.

Lung adenocarcinoma (LUAD) is the main pathological subtype of non-small-cell lung cancer. Endoplasmic reticulum stress (ERS) has been found to be involved in multiple tumor-related biological processes. At present, a comprehensive analysis of ERS-related genes in LUAD is still lacking.

Optimization of Headspace Solid-Phase Micro-Extraction Conditions (HS-SPME) and Identification of Major Volatile Aroma-Active Compounds in Chinese Chive ( Rottler).

In order to rapidly and precisely identify the volatile compounds in Chinese chive ( Rottler), seven key parameters of headspace solid-phase micro-extraction conditions (HS-SPME) from Chinese chive were optimized. A total of 59 volatile compounds were identified by using the optimized method, including 28 ethers, 15 aldehydes, 6 alcohols, 5 ketones, 2 hydrocarbons, 1 ester, and 2 phenols. Ethers are the most abundant, especially dimethyl trisulfide (10,623.

Effects of iron-catalyzed and metmyoglobin oxidizing systems on biochemical properties of yak muscle myofibrillar protein.

The objective of this study was to compare the effects of two oxidation systems on the biochemical properties of yak myofibrillar protein (MP). Oxidation was induced by incubating MP with either an iron-catalyzed oxidizing system (IOS) or a metmyoglobin-oxidizing system (MOS). The following indicators of protein oxidation and protein degradation were analyzed.

[Interaction of Flightless I with Nup88 and Importin β].

High expression of Fightless I (FLII) is associated to multiple tumors. Based on our previous study that FLII might be involved in the nuclear export, we assessed the possible interaction of FLII with the nuclear envelop associating proteins Importin β and Nup88. We first constructed GST-FLII, GST-LRR recombinant plasmids and transformed them into the Rosetta strain to produce GST-FLII, GST-LRR fusion protein.

Cytoplasmic hnRNPK interacts with GSK3β and is essential for the osteoclast differentiation.

Osteoclast differentiation is a complex and finely regulated physiological process that involves a variety of signaling pathways and factors. Recent studies suggested that the Ser9 phosphorylation of Glycogen synthase kinase-3β (GSK3β) is required for the osteoclast differentiation. However, the precise underlying mechanism remains unclear.

A homogeneous time-resolved fluorescence-based high-throughput screening for discovery of inhibitors of Nef-sdAb19 interaction.

The human immunodeficiency virus (HIV) protein negative factor (Nef) is important for AIDS pathogenesis. An anti-Nef single-domain antibody (sdAb19) derived from camelids has been previously generated and shown to effectively block the physiological functions of Nef in vitro and in vivo in nef-transgenic mice. However, sdAb19 must be ectopically expressed within the target cell to be able to exert its neutralizing effect on Nef, while the extra-cellular administration method turned out to be ineffective.

Unexpectedly strong magnetic anisotropy in a mononuclear eight-coordinate cobalt(II) complex: a theoretical exploration.

Ab initio methods have been used to explore the unexpectedly strong magnetic anisotropy and the magnetostructural correlations in mononuclear eight-coordinate complex [Co(II)(12-crown-4)2](2+). Our calculations showed that both decreasing α and increasing φ may enhance its magnetic anisotropy, which was rationalized by the qualitative theory proposed by Long and co-workers. Moreover, we deduced that the |D| value of [Co(II)(12-crown-4)2](2+) with α = 52° and φ = 43° is the largest one.

Slow magnetic relaxation in a mononuclear eight-coordinate cobalt(II) complex.

The quest for the single-molecular magnets (SMMs) based on mononuclear transition-metal complexes is focused on the low-coordinate species. No transition-metal complex with a coordination number of eight has been shown to exhibit SMM properties. Here the magnetic studies have been carried out for a mononuclear, eight-coordinate cobalt(II)-12-crown-4 (12C4) complex [Co(II)(12C4)2](I3)2(12C4) (1) with a large axial zero-field splitting.

Determination of 15 sedative residues in mutton by rapid resolution liquid chromatography-tandem mass spectrometry.

Background: The use of xenobiotic compounds in animal husbandry has given rise to consumer anxieties regarding residual risk and food safety. Thus, animal tissues have become main samples for residue analysis and food safety for sedatives. In this study, a rapid resolution liquid chromatography-tandem mass spectrometry (RRLC-MS/MS) method was established for the determination of 15 sedatives residues in mutton.

The Global Volatile Signature of Veal via Solid-phase Microextraction and Gas Chromatography-mass Spectrometry.

The volatile composition of veal has yet to be reported and is one of the important factors determining meat character and quality. To identify the most important aroma compounds in veal from Holstein bull calves fed one of three diets, samples were subjected to solid-phase microextraction (SPME) combined with gas chromatography-quadrupole mass spectrometry (GC-MS). Most of the important odorants were aldehydes and alcohols.

New method for the simultaneous analysis of types A and B trichothecenes by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry in potato tubers inoculated with Fusarium sulphureum.

A reliable and sensitive method for rapid simultaneous determination of two type A (T-2 and diacetoxyscirpenol) and two type B (3-acetyldeoxynivalenol and Fusarenon X) trichothecenes was developed and successfully applied for detecting trichothecenes in potato tubers by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry. The established method was further evaluated by determining the linearity (R ≥ 0.9995), recovery (113.