Publications by authors named "Jinmao Xiong"
The use of avian herpesviruses (Marek's disease virus, MDV) as vectors to express the capsid protein of infectious bursal disease virus (IBDV) was well established, and its protection against IBDV challenge has been evaluated previously. However, there is little data about rMDV1 expressing the VP2 protein of IBDV protecting SPF and commercial chickens against virulent IBDV (vIBDV) challenge. In this study, we constructed a stable rMDV1 expressing the VP2 protein of IBDV by inserting the coding sequence within the US10 gene of MDVl by homologous recombination and designated this as rMDVl-US10L, and evaluated effectiveness of the recombinant VP2 protein with SPF chickens and commercial chickens with maternal antibodies in vIBDV challenge.
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- Recent research indicates that increased populations of mast cells and tryptase activity contribute to lesion development in immune organs of chickens infected with very virulent infectious bursal disease virus (vvIBDV).
- The study utilized ketotifen, a mast cell stabilizer, to analyze changes in mast cell numbers, tryptase, and histamine in the bursa of Fabricius after infection, finding significant increases in infected birds compared to controls.
- Results showed that ketotifen treatment reduced mast cell activity and associated inflammatory responses, suggesting a potential method to modulate injury caused by vvIBDV through inhibition of mast cell degranulation.
The potential role of the mast cells in the invasion of very virulent infectious bursal disease virus (vvIBDV) is unknown. We evaluated mast cell activity and tryptase production after vvIBDV infection in special pathogen-free (SPF) chickens using cytochemistry and immunohistochemistry analyses. The results were as follows: (1) severe histologic lesions were observed in the thymus, spleen, cloacal bursa, liver, kidney and other tissues.
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