Actin Dysregulation Mediates Nephrotoxicity of Cassiae Semen Aqueous Extracts.

semen, commonly consumed as roasted tea, has been widely used for both medicinal purposes and dietary supplements. In this study, we investigated the nephrotoxic effects and underlying mechanisms of semen aqueous extracts (CSAEs) using computational and animal models. Both male and female Sprague Dawley rats were treated with 4.

Target-induced multiregion MNAzyme nanowires for ultrasensitive homogeneous detection of microRNAs.

A target-induced multiregion MNAzyme nanowire system is designed for the ultrasensitive and homogeneous detection of microRNAs (miRNAs). miRNA-21 and miRNA-375 are chosen as analytes, and a miRNA-induced primer exchange reaction (PER) is utilized to construct a long DNA strand with repetitive sequences. This innovative design enables the efficient anchoring of numerous MNAzymes.

[Highly sensitive detection of fluorescent whitening agents in flour using sheathless capillary electrophoresis-electrospray ionization-tandem mass spectrometry].

Fluorescent whitening agents (FWAs) are dyes that emit visible blue or blue-purple fluorescence upon ultraviolet-light absorption. Taking advantage of light complementarity, FWAs can compensate for the yellow color of many substances to achieve a whitening effect; thus, they are used extensively in various applications. FWAs are generally stable, but their presence in the environment can lead to pollution and accumulation in the body through the food chain.

Safety, tolerability, pharmacokinetics, and pharmacodynamics of TG103 injection in participants who are overweight or obese: a randomized, double-blind, placebo-controlled, multiple-dose phase 1b study.

Background: TG103, a glucagon-like peptide-1 analog, is being investigated as an option for weight management. We aimed to determine the safety, tolerability, pharmacokinetics, and pharmacodynamics of TG103 injection in participants who are overweight or obese without diabetes.

Methods: In this randomized, double-blind, placebo-controlled, multiple-dose phase 1b study, participants aged 18-75 years with a body-mass index (BMI) ≥ 26.

Arterial embolization in the treatment of multiple renal and hepatic hamartomas with spontaneous hemorrhage and 2-year follow-up: a case report.

Background: Hamartoma is a common benign tumor that usually occurs in the kidney, liver, lung, and pancreas. Large renal hamartomas may spontaneously rupture and hemorrhage, which is potentially life-threatening.

Case Presentation: This report describes a 46-year-old Han Chinese female patient with multiple renal and hepatic hamartomas with rupture and hemorrhage of giant hamartoma in the left kidney.

Hydroxyl silicone oil grafting onto a rough thermoplastic polyurethane surface created durable super-hydrophobicity.

Indwelling medical catheters are frequently utilized in medical procedures, but they are highly susceptible to infection, posing a vital challenge for both health workers and patients. In this study, the superhydrophobic micro-nanostructure surface was constructed on the surface of thermoplastic polyurethane (TPU) membrane using heavy calcium carbonate (CaCO) template. To decrease the surface free energy, hydroxyl silicone oil was grafted onto the surface, forming a super-hydrophobic surface.

Paper-based chiral biosensors using enzyme encapsulation in hydrogel network for point-of-care detection of lactate enantiomers.

Chiral analysis is of pivotal importance in a variety of fields due to the different biological activities and functions of enantiomers. Here, we develop a simple paper-based chiral biosensor that can perform sample-to-answer simultaneous analysis of lactate enantiomers in human serum samples. By modification of alginate hydrogel with "egg-box" three-dimensional network structure on a glass microfiber paper, reagents of enantiomer-selective enzymatic reactions are efficiently encapsulated forming the sensing regions for chiral analysis.

A comparative metabolomics study between grain-sized moxibustion and suspended moxibustion on rats with gastric ulcers.

Grain-sized moxibustion (GS-Moxi) and suspended moxibustion (S-Moxi) represent the two typical local heat therapies in Traditional Chinese Medicine (TCM) and have been extensively used in treating gastric ulcers (GU) in China. However, the difference in biological response between the two moxibustion therapies in treating GU remains unclear. Here we investigated the therapeutic effect and potential mechanistic difference underlying the two moxibustion methods.

Dual-mode DNA walker-based optical fiber biosensor for ultrasensitive detection of microRNAs.

We present a novel dual-mode DNA-walker based optical fiber biosensor (DMDW-Opt biosensor) for sensitive assay of micro-RNAs in bio-samples. In the sensor system, we develop a new strategy for the cascade amplification, DNA-walker/rolling cycle amplification (RCA), by the use of the residue track of the walker. The strategy can significantly improve the response of the sensor and avoid any tedious operation procedure.

Serotonin acts through YAP to promote cell proliferation: mechanism and implication in colorectal cancer progression.

Serotonin, also known as 5-hydroxytryptamine (5-HT), is a key messenger that mediates several central and peripheral functions in the human body. Emerging evidence indicates that serotonin is critical in tumorigenesis, but its role in colorectal cancer remains elusive. Herein, we report that serotonin transporter (SERT) transports serotonin into colorectal cancer cells, enhancing Yes-associated protein (YAP) expression and promoting in vitro and in vivo colon cancer cell growth.

Biological role of long non-coding RNA FTX in cancer progression.

Long non-coding RNAs (LncRNAs) are involved in several types of cancer and participate actively in tumorigenesis and disease progression. LncRNA FTX is the transcription product of the FTX gene located at the X-inactivation center (Xic). LncRNA FTX has been shown to regulate cancer cell proliferation, migration, and aberrant metabolism, as well as increase tumor growth and metastasis in vivo.

Capillary-based fluorescence microsensor with polyoxometalates as nanozyme for rapid and ultrasensitive detection of artemisinin.

A novel capillary-based fluorescence microsensor for artemisinin was developed with functional polyoxometalates (POMs) as nanozyme by a layer-by-layer self-assembly strategy. Vanadomolybdophosphoric heteropoly acid (HPMoVO, PMoV) and tungstophosphoric heteropoly acid (NaPWOCu, PWCu) with high peroxidase-like activity were synthesized and immobilized on capillary to catalyze artemisinin/thiamine reaction and generate the amplified fluorescence signal. The wide linear range up to 13.

[Nuclear magnetic resonance-based metabolomics revealed metabolite profiles participate in intervention effect of refined moxibustion in gastric ulcer model rats].

Objective: To investigate the effect of refined moxibustion on expression of gastric mucosal epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF), and changes of metabolite profiles in gastric ulcer (GU) rats, so as to analyze its mechanism underlying improvement of GU.

Methods: Male SD rats were randomized into control, model, acupoint moxibustion groups (=6 per group). The GU model was induced by cold-restraint stress.

Predicting the potential toxicity of 26 components in semen using and approaches.

semen are dried and ripe seeds of L. or L. (Fabaceae) and have been made into roasted tea or used as a traditional medicine in Asian countries.

[Endogenous metabolites involving effects of moxibustion on injured gastric mucosal tissue in rats with stomach heat or cold syndrome based on nuclear magnetic resonance spectroscopy].

Objective: To investigate the profile of metabolites of gastric mucosa involving the effectiveness of moxibustion in the treatment of syndromes of stomach heat (SH) and stomach cold (SC) by H-nuclear magnetic resonance (H-NMR) spectroscopy in rats, so as to reveal its mechanisms underlying improvement of gastric disorders.

Methods: Male SD rats were randomly divided into control, SH-model, SC-model, SH-moxibustion and SC-moxibustion groups (＝6 rats/group). The SH-model and SC-model were established by gavage of pepper liquid plus ethanol, and ice water plus NaOH, respectively.

Anchoring copper nanoclusters to Zn-containing hydroxy double salt: construction of 2D surface confinement induced enhanced emission toward bio-enzyme sensing and light-emitting diode fabrication.

Surface CIEE based on Zn-HDS as host material and GSH-CuNCs as guest molecules was developed to produce fluorescence composite GSH-CuNCs/Zn-HDS for the first time. It displays high quantum yield, long fluorescence lifetime and good stability, and was applied to sensitive bioenzyme sensing and fabrication of a high performance light-emitting diode.

Investigation of the surface confinement effect of copper nanoclusters: construction of an ultrasensitive fluorescence turn-on bio-enzyme sensing platform.

Copper nanoclusters (CuNCs) have attracted considerable research interest due to their good physicochemical properties, ease of preparation, and low price. However, the low quantum yield and poor stability in aqueous solutions have greatly limited their applications. In order to improve the fluorescence properties and stability of CuNCs, in this paper, the surface confinement effect of CuNCs based on 2D layered double hydroxide (LDH) was proposed to prepare the fluorescent composites of glutathione protected CuNCs and LDH (GS-CuNCs/LDH) with excellent quantum yield and long fluorescence lifetime.

Effect of euphorbia factor L1 on oxidative stress, apoptosis, and autophagy in human gastric epithelial cells.

Background: Euphorbia factor L1 (EFL1), a lathyrane-type diterpenoid from the medicinal herb Euphorbia lathyris L. (Euphorbiaceae), has been reported for many decades to induce gastric irritation, but the underlying mechanisms remain unclear.

Purpose: The objective of this study was to investigate EFL1-induced cytotoxicity and the potential mechanisms of action on the human normal gastric epithelial cell GES-1.

Anthraquinones in the aqueous extract of Cassiae semen cause liver injury in rats through lipid metabolism disorder.

Background: Cassiae semen has been used as the tea or medicine component to treat hyperlipidemia or for hepatoprotection. However, Cassiae semen was reported to be a potentially hepatotoxic herb, and the underlying hepatotoxicity mechanisms or specific hepatotoxic components of Cassiae semen are unknown.

Purpose: In this study, we aimed to explore the potential hepatotoxicity mechanisms and the hepatotoxic components of Cassiae semen.

Electrostatically controlled fluorometric assay for differently charged biotargets based on the use of silver/copper bimetallic nanoclusters modified with polyethyleneimine and graphene oxide.

An electrostatically controlled fluorometric assay is described that is based on the use of silver/copper bimetallic nanoclusters. The nanoclusters were coated with polyethyleneimine (PEI-Ag/CuNCs). At pH 7.

[Toxicokinetics of bakuchiol, hepatic and renal toxicity in rats after single oral administration of Psoraleae Fructus and combination with Glycyrrhizae Radix et Rhizoma].

To study the toxicokinetics of bakuchiol, hepatic and renal toxicity in rats after single oral administration of Psoraleae Fructus and combined with Glycyrrhizae Radix et Rhizoma, in order to provide scientific evidences for clinical safe medication use. A total of 35 SD rats were randomly divided into seven groups: vehicle (distilled water) control group, Glycyrrhizae Radix et Rhizoma group, positive control (aristolochic acid A) group, Psoraleae Fructus (40 g x kg(-1)) group( both male and female rats), Psoraleae Fructus and Glycyrrhizae Radix et Rhizoma (40 +20) g x kg(-1) group (both male and female rats). HPLC-UV method was used to determine the concentration of bakuchiol in rat plasma at different time points after single oral administration.

Transcriptome-based gene profiling provides novel insights into the characteristics of radish root response to Cr stress with next-generation sequencing.

Radish (Raphanus sativus L.) is an important worldwide root vegetable crop with high nutrient values and is adversely affected by non-essential heavy metals including chromium (Cr). Little is known about the molecular mechanism underlying Cr stress response in radish.