induces MST2 phosphorylation mediating the activation of hippo signaling pathway to promote apoptosis and lung tissue damage.

() is an intracellular parasite, and its regulation of host cell apoptosis directly affects its parasitism. Studies link -induced apoptosis to abnormal expression of mammalian STE20-like protein kinase 2 (MST2), but its precise role remains unclear. In this study, the regulatory roles in apoptosis and pathogenicity of infection were identified and .

Elevation and distraction of the Tibial periosteum in the management of chronic ischemic lower limb diseases.

Objective: This study investigates the effectiveness of tibia periosteum distraction (TPD) applied to the tibial periosteum, an innovative approach grounded in Ilizarov's tension-stress theory, for the treatment of ischemic diabetic foot and vasculitic foot conditions.

Methods: A retrospective analysis was conducted on 33 patients (36 limbs) who underwent TPD between June 2019 and May 2022. The study comprised 21 males (23 limbs) and 12 females (13 limbs), aged 41 to 80 years (mean age: 63.

MicroRNA expression profile of chicken liver at different times after Histomonas meleagridis infection.

Histomonas meleagridis, an anaerobic intercellular parasite, is known to infect gallinaceous birds, particularly turkeys and chickens. The resurgence of histomonosis in recent times has resulted in significant financial setbacks due to the prohibition of drugs used for disease treatment. Currently, research on about H.

Phosphoproteomic analysis reveals changes in A-Raf-related protein phosphorylation in response to Toxoplasma gondii infection in porcine macrophages.

Background: Toxoplasma gondii is an obligate intracellular protozoan parasite that causes severe threats to humans and livestock. Macrophages are the cell type preferentially infected by T. gondii in vivo.

Organic Geochemical Evidence for the Formation of Condensate from Coaly Source Rocks in the Wumaying Buried Hill of the Huanghua Depression, Bohai Bay Basin.

Although oil and gas from coaly source rocks have been widely discovered worldwide, the role of oil generated from coal measures in marine-continental coaly deposits during the Carboniferous-Permian period in the Bohai Bay Basin has long been a subject of debate. The recent discovery of a condensate reservoir in the Wumaying buried hill within the Huanghua Depression of the Bohai Bay Basin offers new potential insights into this issue. In this study, we employed organic geochemical methods to explore the possibility of the Carboniferous-Permian coal deposit being a primary source of the condensate.

Localization confirms EnApiAP2 protein encoded by as a nuclear protein in .

Introduction: Apicomplexan AP2 family of proteins (ApiAP2) are transcription factors (TFs) that regulate parasite growth and development, but little is known about the ApiAP2 TFs in spp. sequence is predicted to encode a ApiAP2 protein (EnApiAP2).

Methods: The cDNAs encoding full-length and truncated EnApiAP2 protein were cloned and sequenced, respectively.

Prevalence and risk factors associated with gastrointestinal parasites in goats () and sheep () from three provinces of China.

Gastrointestinal (GI) parasites in small ruminants, especially goats and sheep, have caused significant socio-economic and public health challenges worldwide. The aim of the present study was to investigate the diversity and prevalence of GI parasites in goats and sheep in Jiangsu, Shaanxi and Hunan provinces of China, and to assess whether the age of animals, sampling season and feeding mode influence the distribution and infection of GI parasites. A total of 1,081 fecal samples collected from goats ( = 835) and sheep ( = 246) were detected by saturated saline flotation technique and nylon sifter elutriation and sieving method for eggs/oocysts, respectively.

Toxoplasma gondii infection regulates apoptosis of host cells via miR-185/ARAF axis.

Background: Toxoplasmosis is a zoonosis with a worldwide presence that is caused by the intracellular parasite Toxoplasma gondii. Active regulation of apoptosis is an important immune mechanism by which host cells resist the growth of T. gondii or avoid excessive pathological damage induced by this parasite.

Identification and Characterization of α-Actinin 1 of and Its Potential Vaccine Candidates against Histomonosis.

is a protozoan parasite that causes histomonosis in gallinaceous birds such as turkeys and chickens. Since the banning and restricted usage of effective drugs such as nitarsone, 80-100% morbidity and mortality occur in turkeys and 20-30% mortality in chickens. New ideas are needed to resolve the re-emergence of histomonosis in poultry.

iTRAQ-based proteomic analysis reveals invasion-related proteins among three developmental stages of Eimeria necatrix.

Eimeria necatrix is an obligate intracellular parasite that has a complex life cycle and causes significant economic losses to the poultry industry. To better understand the cellular invasion mechanism of E. necatrix and develop new measures against its infection, we conducted isobaric tags for relative and absolute quantitation (iTRAQ) proteomic analysis to investigate protein abundance across different life cycle stages, including unsporulated oocysts (UO), sporozoites (SZ) and second-generation merozoites (MZ-2).

Examination of gametocyte protein 22 localization and oocyst wall formation in Eimeria necatrix using laser confocal microscopy and scanning electron microscopy.

Background: Eimeria parasite infection occurs via ingestion of oocysts. The robust, bilayer oocyst wall is formed from the contents of wall-forming bodies (WFBs), WFB1 and WFB2, located exclusively in macrogametocytes. Eimeria necatrix gametocyte proteins 22 and 59 (EnGAM22 and EnGAM59) have been found to localize to WFBs and the oocyst wall.

Tandem mass tag-based quantitative proteomics analyses of a chicken-original virulent and its attenuated strain in China.

Introduction: can cause histomonosis in poultry. Due to the prohibition of effective drugs, the prevention and treatment of the disease requires new strategies. Questions about its pathogenic mechanisms and virulence factors remain puzzling.

Retrieval of sound-velocity profile in ocean by employing Brillouin scattering LiDAR.

Accurate remote sensing of the sound velocity profile of the upper-ocean mixed layers is of major important in oceanography, especially in underwater acoustic communication. However, the existing technologies cannot realize fast and real-time detection on sound velocity profile, a cost efficiency, flexibility, and real-time remote sensing technique is still highly urgent. In this paper, we propose a novel approach based on stimulated Brillouin scattering (SBS) LiDAR for retrieving the sound velocity profile.

MicroRNA expression profile of chicken cecum in different stages during Histomonas meleagridis infection.

Background: Histomonas meleagridis is an anaerobic, intercellular parasite, which infects gallinaceous birds such as turkeys and chickens. In recent years, the reemergence of Histomoniasis has caused serious economic losses as drugs to treat the disease have been banned. At present, H.

A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David's deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China.

Background: Eimeria coccidiosis is a significant intestinal parasitic disease, which can lead to weight loss, disease and even death of many animals. At present, there is no information about the prevalence of Eimeria among the world's endangered species of Père David's deer (Elaphurus davidianus). Therefore, the purpose of this study is to identify an unknown Eimeria genus in the Père David's deer in Dafeng Milu National Nature Reserve, China.

Evaluation of Shear Capacity of Steel Fiber Reinforced Concrete Beams without Stirrups Using Artificial Intelligence Models.

The shear transfer mechanism of steel fiber reinforced concrete (SFRC) beams without stirrups is still not well understood. This is demonstrated herein by examining the accuracy of typical empirical formulas for 488 SFRC beam test records compiled from the literature. To steer clear of these cognitive limitations, this study turned to artificial intelligence (AI) models.

Global MicroRNAs Expression Profile Analysis Reveals Possible Regulatory Mechanisms of Brain Injury Induced by Infection.

() is an obligate intracellular parasitic protozoan that can cause toxoplasmosis in humans and other endotherms. can manipulate the host gene expression profile by interfering with miRNA expression, which is closely associated with the molecular mechanisms of -induced brain injury. However, it is unclear how manipulates the gene expression of central nervous system (CNS) cells through modulation of miRNA expression during acute and chronic infection.

Cluster analysis of splenocyte microRNAs in the pig reveals key signal regulators of immunomodulation in the host during acute and chronic Toxoplasma gondii infection.

Background: Toxoplasma gondii is an obligate intracellular protozoan parasite that can cause a geographically widespread zoonosis. Our previous splenocyte microRNA profile analyses of pig infected with T. gondii revealed that the coordination of a large number of miRNAs regulates the host immune response during infection.

Failure Criteria and Constitutive Relationship of Lightweight Aggregate Concrete under Triaxial Compression.

This paper investigates the compression behavior and failure criteria of lightweight aggregate concrete (LAC) under triaxial loading. A total of 156 specimens were tested for three parameters: concrete strength, lateral confining pressure and aggregate immersion time, and their effects on the failure mode of LAC and the triaxial stress-strain relationship of LAC is studied. The research indicated that, as the lateral constraint of the specimen increases, the failure patterns change from vertical splitting failure to oblique shearing failure and then to indistinct traces of damage.

Influence of temperature-salinity-depth structure of the upper-ocean on the frequency shift of Brillouin LiDAR.

Brillouin-based LiDAR is an alternative remote sensing technique for measuring the distribution profiles of temperature, salinity, and sound speed in the upper ocean mixed layer. Its principle is based on the dependence of Brillouin frequency shift on the temperature, salinity, and depth of ocean. Therefore, it is necessary to investigate the effect of various seawater parameters on Brillouin frequency shift for ocean remote sensing by using the Brillouin LiDAR.