Cyanidin-3-O-β-glucoside, a Natural Polyphenol, Exerts Proapoptotic Effects on Activated Platelets and Enhances Megakaryocytic Proplatelet Formation.

This study investigated whether the anthocyanin cyanidin-3-O-β-glucoside (Cy-3-g) could affect platelet apoptosis and proplatelet formation in vitro. Thrombin-stimulated or resting human platelets and Meg-01 megakaryocytes were incubated with Cy-3-g (0, 0.5, 5, or 50 μM).

Plant food anthocyanins inhibit platelet granule secretion in hypercholesterolaemia: Involving the signalling pathway of PI3K-Akt.

Controlling platelet granule secretion has been considered an effective strategy to dampen thrombosis and prevent atherosclerosis. Anthocyanins are natural plant pigments and possess a wide range of biological activities, including cardiovascular protective activity. In the present study we explored the effects and the potential mechanisms of anthocyanins on platelet granule secretion in hypercholesterolemia.

[Differential expression on cell wall formative genes in cell suspension cultures of Populus tomentosa Carr].

With fine and granular suspension cultures established from P. tomentosa, differential expression of 12 genes related to cell wall formation including cell expansion, cellulose synthesis, lignin synthesis, etc, were investigated by reverse transcript PCR (RT-PCR) technique. Fine suspension cells showed high expression on 3 alpha-Exp genes with the highest alpha-Exp2 followed by alpha-Exp3 and alpha-Exp1 suggesting their strong cell expansion, while granular suspension cells only exist high expression on alpha-Exp3 indicating their later period of cell expansion or initial stage of secondary cell wall formation.

[Isolation and characterization of nuclear microsatellites from Chamaecypress obtusa Endl].

Using 300 approximately 1 000 bp DNA fragments retrieved from 2% agarose gel electrophoresis on nuclear DNA samples of Chamaecypress obtusa with Nde II restricted digestion, microsatellites were efficiently isolated by using Dynabeads M280 streptavitin beads. These beads could immobilize hybrid products between biotin-labeled (CT) 15 probe, and 300 approximately 1 000 bp DNA fragments previously linkaged to Sau3A I cassette. Two enriched microsatellite libraries with about 2 200 positive white clones were constructed by inserting potential microsatellite fragments into vector pUC118 BamH I /BAP and culturing with host E.