Biochim Biophys Acta Mol Cell Biol Lipids
November 2024
Nonalcoholic fatty liver disease (NAFLD) progression is relevant to oxidative stress, while NADPH oxidase can produce ROS. This study explored how the upstream stimulatory factor 1 (USF1) regulates cytochrome b-245 alpha chain (CYBA) expression through the NADPH-ROS pathway and its impact on oxidative stress and lipid accumulation in NAFLD. Bioinformatics analysis identified CYBA as a gene with altered expression in NAFLD.
View Article and Find Full Text PDFElectrochemical-modulated surface-enhanced Raman scattering (EC-SERS) integrates the benefits of SERS with electrochemical techniques. By controlling the electrode potential, Raman spectroscopy allows for the analysis of molecules with enhanced sensitivity and selectivity. With its large volume and high sample consumption, the traditional three-electrode electrochemical cell constrains the widespread adoption of EC-SERS.
View Article and Find Full Text PDFSci Total Environ
November 2024
Extreme precipitation can significantly influence the water quality of surface waters. However, the total amount of bacteria carried by rainfall runoff is poorly understood. Here, thirty rainfall scenarios were simulated by artificial rainfall simulators, with designed rainfall intensity ranging from 19.
View Article and Find Full Text PDFDissolved oxygen (DO) levels and carbon-to-nitrogen (C/N) ratio affect nitrous oxide (NO) emissions by influencing the physiological and ecological dynamics of nitrifying and denitrifying microbial communities in activated sludge systems. For example, Nitrosomonas is a common NO producing nitrifying bacteria in wastewater treatment plants (WWTPs), and DO conditions can affect the NO production capacity. Previous studies have reported NO emission characteristics under adequate DO and C/N conditions in A/O WWTPs.
View Article and Find Full Text PDFBackground: Circular RNAs (circRNAs) are involved in the progression of osteoporosis; however, their impact on osteogenic differentiation has yet to be fully elucidated. In this study, we identified a novel circRNA known as circZfp644-205 and investigated its effect on osteogenic differentiation and apoptosis in osteoporosis.
Methods: CircZfp644-205, miR-445-3p, and SMAD2 levels were measured using quantitative real-time polymerase chain reaction (qRT-PCR).
Ciprofloxacin (CIP) poses a high risk of resistance development in water environments. Therefore, comprehensive effects and recovery strategies of CIP in anaerobic ammonia oxidation (anammox) process were systematically elucidated from consortia and pure strains perspectives. The anammox consortia was not significantly affected by the stress of 10 mg L CIP, while the higher concentration (20 mg L) of CIP caused a dramatic reduction in the nitrogen removal performance of anammox system.
View Article and Find Full Text PDFObjectives: The aim of the study was to evaluate the efficacy and safety of allogeneic umbilical cord-derived mesenchymal stem cells (TH-SC01) for complex perianal fistula in patients with Crohn's disease (CD).
Methods: This was an open-label, single-arm clinical trial conducted at Jinling Hospital. Adult patients with complex treatment-refractory CD perianal fistulas (pfCD) were enrolled and received a single intralesional injection of 120 million TH-SC01 cells.
The use of seawater to alleviate water shortages causes an increase of salinity in municipal pipe networks, posing challenges for biological wastewater treatment. The impacts of two compatible solutes on the anammox process under salt stress (20 g L) were compared here at the genetic and microbial levels. The findings revealed that both 0.
View Article and Find Full Text PDFRemotely sensed imagery is used as a tool to aid decision makers and scientists in a variety of fields. A recent world event in which satellite imagery was extensively relied on by a variety of stakeholders was the COVID-19 pandemic. In this article we aim to give an overview of the types of information offered through remote sensing (RS) to help address different issues related to the pandemic.
View Article and Find Full Text PDFAs a key component of extracellular polymeric substances (EPS), extracellular deoxyribonucleic acid (eDNA) acts as a bridge in maintaining the structural stability of granular sludge. However, its ability of carrying antibiotic resistance genes (ARGs) promotes the gene horizontal transfer, raising a high risk for human health. In this study, a series of batch tests were performed to elucidate the response of anammox granular sludge (AnGS) with different sizes (S-AnGS with diameters lower than 0.
View Article and Find Full Text PDFLong non-coding RNA ELFN1 antisense RNA 1 (ELFN1-AS1) has been reported as a cancer driver in many human malignancies. This study was conducted to investigate the function of ELFN1-AS1 in gastric cancer (GC) and its mechanism of action. Bioinformatics analysis revealed increased expression of ELFN1-AS1 in GC, and abundant expression of ELFN1-AS1 was observed in the acquired GC cell lines.
View Article and Find Full Text PDFAntibiotics and antibiotic resistance genes (ARGs) have been recognized as emerging high-risk pollutants for human and animal health. This study systematically investigated the comprehensive effects of a typical antibiotic (sulfadimidine, SDM) in livestock and poultry breeding wastewater on the anammox process, with the aim of elucidating the intracellular and extracellular protective mechanisms of the anammox consortia to the antibiotic stress. Results revealed that the high-concentration SDM significantly reduced the specific anammox activity (SAA) by 37.
View Article and Find Full Text PDFBackground: Increasing evidence implicates circular RNAs (circRNAs) have been involved in human cancer progression. However, the mechanism remains unclear. In this study, we identified novel circRNAs related to gastric cancer and constructed a circRNA-miRNA-mRNA network.
View Article and Find Full Text PDFUlcerative colitis (UC) is a significant threat to human life. Hence, there is an urgent requirement to understand the mechanism of UC progression and to develop novel therapeutic interventions for the treatment of UC. The present study aimed to evaluate the potential significance of long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) in the progression of UC.
View Article and Find Full Text PDFIt has been reported that knockdown of circular RNA (circ) ATPase class II type 9B (Atp9b) can reduce lipopolysaccharide (LPS)-induced inflammation, which plays a notable role in ulcerative colitis (UC). The present study aimed to explore the role of circAtp9b in UC. The expression levels of Atp9b and PTEN in the plasma of patients with UC (n=60) and healthy controls (n=60) were determined via reverse transcription-quantitative PCR.
View Article and Find Full Text PDFThe effects of multiple antibiotics on the anaerobic ammonia oxidation (anammox) process were investigated. The resistance of the anammox system to high-concentration antibiotics was also demonstrated through gradual acclimation experiments. Inhibition of the anammox process (R) occurred when the concentrations of erythromycin (ERY), sulfamethoxazole (SMX) and tetracycline (TC) were 0.
View Article and Find Full Text PDFSci Total Environ
January 2022
The anaerobic ammonium oxidation (anammox) process has been recognized as an efficient nitrogen removal technology. However, anammox bacteria are susceptible to surrounding environments and different pollutants, which limits the extensive application of the anammox process worldwide. Numerous researchers investigate the effects of various pollutants on the anammox process or bacteria, and related findings have also been reviewed with the focused on their inhibitory effects on process performance and microbial community.
View Article and Find Full Text PDFThe anaerobic ammonium oxidation (anammox) process is regarded as a promising approach to treat antibiotic-containing wastewater. Therefore, it is urgent to elucidate the effects of various antibiotics on the anammox process. Moreover, the mechanism of extracellular polymeric substance (EPS) as protective barriers to relieve antibiotic stress remain unclear.
View Article and Find Full Text PDFThe function and possible mechanism of lncRNA Small Nucleolar RNA Host Gene 3 (SNHG3) in GC have not been fully studied. The aim of our study was to investigate the role of SNHG3 in the proliferation, migration, and invasion of GC cell lines. The expressions of SNHG3, miR-326, and TWIST in GC9811-P GC cell lines were detected by RT-qPCR.
View Article and Find Full Text PDFmiR-17-5p has been proved that play important roles in many kinds of tumors progression. This study aimed at explore the function and mechanism of miR-17-5p in thyroid cancer (TC). RT-qPCR was used to detect miR-17-5p and Early growth response 2 (EGR2) expression in TC tissues and cells.
View Article and Find Full Text PDFTwo types of anaerobic ammonium oxidation (anammox) seed sludge were selected to evaluate their responses to copper nanoparticles (CuNPs) exposure. Antibiotic-exposed anammox granules (R) were more likely to be inhibited by 5.0 mg L CuNPs than the normal anammox granules (C).
View Article and Find Full Text PDFAntibiotic pollution is becoming increasingly severe due to its extensive use. The potential application of the anaerobic ammonium oxidation (anammox) process in the treatment of wastewater containing antibiotics has attracted much attention. As common antibiotics, spiramycin (SPM) and streptomycin (STM) are widely used to treat human and animal diseases.
View Article and Find Full Text PDFPurpose: To evaluate the complete response (CR) rate, recurrence rate and pregnancy outcome of complex endometrial hyperplasia (CEH) and early endometrial carcinoma (EC) patients treated with hysteroscopic surgery combined with progesterone, and to analyze the related influencing factors for prognosis.
Methods: The clinical data of 96 patients histopathologically diagnosed with CEH or early EC and treated with hysteroscopic surgery combined with levonorgestrel-releasing intrauterine system (LNG-IUS) and/or high-efficient megestrole acetale (MA) in our hospital from January 2014 to January 2016 were retrospectively analyzed. The hysteroscopic examination was performed for patients, and the improvement of endometrium was evaluated via curettage every 3 months after treatment.