MKAN-MMI: empowering traditional medicine-microbe interaction prediction with masked graph autoencoders and KANs.

The growing microbial resistance to traditional medicines necessitates in-depth analysis of medicine-microbe interactions (MMIs) to develop new therapeutic strategies. Widely used artificial intelligence models are limited by sparse observational data and prevalent noise, leading to over-reliance on specific data for feature extraction and reduced generalization ability. To address these limitations, we integrate Kolmogorov-Arnold Networks (KANs), independent subspaces, and collaborative decoding techniques into the masked graph autoencoder (Mask GAE) framework, creating an innovative MMI prediction model with enhanced accuracy, generalization, and interpretability.

Clinical analysis of special types of tracheobronchial foreign bodies in children.

Objectives: To explore the clinical diagnosis and treatment of special types of tracheobronchial foreign bodies in children and provide a reference for clinicians to formulate treatment plans.

Methods: Clinical data of 29 children with special types of tracheobronchial foreign bodies who were treated at The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University between June 2017 and June 2022 were collected and analyzed, and their diagnosis and treatment processes were reviewed.

Results: All 29 special types of foreign bodies were successfully removed using rigid bronchoscopy under general anesthesia, with no surgical complications.

Low-Temperature Plasma Radiofrequency Ablation for Congenital Epiglottic Cysts.

Congenital epiglottic cysts are rare disorders of the larynx with symptoms such as laryngeal stridor and inspiratory dyspnea and are life-threatening in severe cases. This study aimed to investigate the usefulness of low-temperature plasma radiofrequency ablation for congenital epiglottic cysts and provide a reference for clinicians to develop treatment options. The clinical data of children (n = 7, 4 males and 3 females) with congenital epiglottic cysts, who were admitted to the Second Affiliated Hospital of Wenzhou Medical University and Yuying Children's Hospital from March 2018 to March 2023, were analyzed retrospectively.

Acceptance Factors and Psychological Investigation of Clinical Trials in Cancer Patients.

Aim: To understand the degree of oncology patients' awareness of drug clinical trials and oncology patients' willingness to participate in drug clinical trials and the factors influencing them.

Methods: The differences in the relevant variables of patients' willingness to accept clinical trials were analyzed, and a descriptive analysis was done for the measurement data (mean and standard deviation). Pearson's correlation coefficient analysis was used to examine the correlation between willingness and the demographic variables.

Evolution of pore structure and fractal characteristics of marine shale during electromagnetic radiation.

Electromagnetic radiation has been proposed to non-aqueously stimulate shale formations, which can generate fractures and enhance the porosity of the matrix. The proposed method consumes electricity and thereby possesses significant advantages for sustainable and environmental hydrocarbon production. In this study, we investigate the pore structure variations of marine shale during electromagnetic radiation.

miR‑193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma.

Emerging evidence has indicated that microRNAs (miRs) are involved in the malignant behavior of cancer. The present study explored the role of miR‑193b in the development and metastasis of osteosarcoma. Compared with F4 osteosarcoma cells, which have a relatively low metastatic potential, highly metastatic F5M2 cells exhibited a lower expression of miR‑193b.

Delta opioid receptor agonist attenuates lipopolysaccharide-induced myocardial injury by regulating autophagy.

Background: Previous studies have described the protective effects of DADLE on myocardial injury in sepsis. Recently, autophagy has been shown to be an innate defense mechanism in sepsis-related myocardial injury. However, whether DADLE has an pro-autophagic effect is yet to be elucidated.

Comparative analysis of cluster versus conventional immunotherapy in patients with allergic rhinitis.

The present study aimed to compare the efficacy and safety of subcutaneous immunotherapy with standardized extract administered in conventional and cluster immunotherapy regiments for patients with persistent allergic rhinitis. A total of 60 patients with moderate to severe allergic rhinitis caused by dust mites were treated for 1 year with either conventional immunotherapy (n=30) or cluster immunotherapy (n=30). Nasal conjunctival symptoms and signs were assessed to evaluate the clinical efficacy of the two regimens, and the incidence of local and systemic adverse reactions were also evaluated.

[Myocardial cells and mitochondrial autophagy in sepsis mice induced by lipopolysaccharide].

Objective: To investigate the alterations in the level of myocardial cells and mitochondrial autophagy during myocardial injury in lipopolysaccharide (LPS)-induced septic animal models.

Methods: Male C57BL/J mice were randomly divided into negative control group (NC), LPS treatment groups (6, 12, 24, 36 hours). The LPS treatment groups were subjected to LPS (10 mg/kg) injection intraperitoneally, and the NC group was injected intraperitoneally with the same amount of saline.

[miR-365 inhibits proliferation and promotes apoptosis of SOSP9607 osteosarcoma cells].

Objective: To investigate the effect of miR-365 on the proliferation and apoptosis in SOSP-9607 osteosarcoma cells.

Methods: SOSP-9607 cells were transiently transfected with miR-365 mimic or miR-365 inhibitor which were artificially synthesized. The expression of miR-365 was detected by real-time PCR (qRT-PCR); the cell cycle and apoptosis was analyzed by flow cytometry; the cell proliferation was observed by MTT assay; and the level of KRAS was determined by qRT-PCR and Western blotting.

GSTM1 null genotype is a risk factor for laryngeal cancer.

It remains unclear whether the Glutathione S-transferase M1 (GSTM1) null genotype influence laryngeal cancer development. This study aimed to investigate the interactions among GSTM1 genotype with regard to laryngeal cancer development. We searched online electronic databases (PubMed, EMBASE and CNKI).

[Juvenile onset respiratory papillomatosis: risk factors for severity].

Objective: This study was designed to explore the risk factors associated with severity of juvenile onset recurrent respiratory papillomatosis.

Method: A retrospective study was conducted to study determinants of severe forms of juvenile recurrent onset respiratory papillomatosis. The patients were separated into different groups based on the onset age, the first recurrence of age, the first recurrence of period, gender and incision of tracheal respectively.

[Function study of non-syndromic deafness associated mitochondrial 12S rRNA A839G mutation].

Objective: To investigate the correlation between nonsyndromic deafness and mitochondrial 12s rRNA A839G mutation.

Methods: According to the clinical manifestations of mitochondrial DNA sequencing and analysis to find and determine family containing mitochondrial 12s rRNA A839G mutation. Harvested its family members blood and transferred their lymphocytes into lymphoblastoid cell lines, followed by cells cultured, cell doubling experiment, susceptibility testing, cellular oxygen consumption rate experiment, ROS and mitochondrial membrane potential experimental tests were progressed to explore the correlation between the A839G mutation and nonsyndromic deafness.

[Analysis of genotype-phenotype correlation for GJB2 in 221 non-syndromic deafness probands and their pedigrees].

Objective: To assess the possible genotype-phenotype correlation for GJB2.

Methods: Retrospectively analyzed GJB2 gene mutations with non-syndromic hearing impairment (NSHI) patients and their families audiological data. Individuals were grouped, according to non-truncated mutant (non-truncating, NT) and truncating mutations (truncating, T), into T/T group, T/NT group and NT/NT group.

[Clinical research about brain oxygen metabolism and neuroelectrophysiology during mild hypothermia in patients with severe head injury].

Objective: To observe the changes of brain oxygen metabolism and neuroelectrophysiology after severe brain injury, and the effects of hypothermia on severe brain injury.

Methods: 148 patients with severe brain injury (GCS 3 - 8, admitted within 10 hours from injury) were selected for this study. Patients were divided into 3 groups, Group GCS 7 - 8, Group GCS 5 - 6 and Group GCS 3 - 4.

[Expression and significance of vascular permeability factor (VPF) in inflammatory cells of nasal polyps].

Objective: To study the expression and significance of vascular permeability factor (VPF) in eosinophil, macrophage and plasma cell in nasal polyps.

Method: VPF in 12 nasal polyps was studied by immunohistochemistry, double immune-enzymatic staining technique and double MGG staining technique. Each section was observed under optical microscope.