Conversion of coastal marsh to aquaculture ponds decreased the potential of methane production by altering soil chemical properties and methanogenic archaea community structure.

Coastal wetlands are among the most productive and dynamic ecosystems globally, contributing significantly to atmospheric methane (CH) emissions. The widespread conversion of these wetlands into aquaculture ponds degrades these ecosystems, yet its effects on CH production and associated microbial mechanisms are not well understood. This study aimed to assess the impact of land conversion on CH production potential, total and active soil organic C (SOC) content, and microbial communities.

Mangrove wetland recovery enhances soil carbon sequestration capacity of soil aggregates and microbial network stability in southeastern China.

Mangrove wetlands are highly productive ecosystems in tropical and subtropical coastal zones, play crucial roles in water purification, biodiversity maintenance, and carbon sequestration. Recent years have seen the implementation of pond return initiatives, which have facilitated the gradual recovery of mangrove areas in China. However, the implications of these initiatives for soil aggregate stability, microbial community structure, and network interactions remain unclear.

Sensory-directed isolation and identification of an intense salicin-like bitter compound in infected teas with bird's eye spot disease.

Teas infected with bird's eye spot disease generally exhibited a lingering and long-lasting, salicin-like bitter taste, which was unpalatable to consumers. Sensory-directed isolation processes have been performed in this study to investigate the salicin-like bitter compounds in infected teas. Results showed that infected teas were extracted using a 70% methanol aqueous solution to produce methanol extract, which was then further separated by sequential solvent extraction (SSE) to obtain dichloromethane extract, which contained the salicin-like bitter compounds.

[Method for Japanese encephalitis virus NS3 protease activity analysis and high-throughput screening assay for inhibitors].

Japanese encephalitis virus (JEV) is a single-stranded and positive-sense RNA, which has a single ORF (open reading frame), encoding a polyprotein precursor. Non-structural protein 3 (NS3) plays an important role in processing the polyprotein precursor and has become an important drug target of flavivirus. In this study, NS2BH-NS3 gene was amplified by PCR and subcloned to the prokaryotic expression plasmid, resulting pET30a-NS2BH-NS3.

Biodegradation of bensulphuron-methyl by a novel Penicillium pinophilum strain, BP-H-02.

A fungal strain able to rapidly degrade bensulphuron-methyl (BSM), called BP-H-02, was isolated for the first time from soil that had been contaminated with BSM for several years. BP-H-02 can use BSM as the sole carbon and energy source for growth in a mineral salt medium. Based on morphological and internal transcribed spacer (ITS) analysis, BP-H-02 was identified as Penicillium pinophilum.