Publications by authors named "Jingyu Jiao"
Raman spectroscopy has found widespread usage in monitoring cell culture processes both in research and practical applications. However, commonly, preprocessing methods, spectral regions, and modeling parameters have been chosen based on experience or trial-and-error strategies. These choices can significantly impact the performance of the models.
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- The study focuses on developing an in-line Raman spectroscopy method to monitor key parameters like glucose, lactate, and viable cell density (VCD) during the commercial production of biosimilar adalimumab using Chinese hamster ovary (CHO) cells in a 1500 L bioreactor.
- The researchers optimized Raman calibration models to achieve low prediction errors for glucose, lactate, and VCD, making the method effective for real-time monitoring of the cell culture process.
- Additionally, the study explored how the number of batches used for model calibration affects model performance, providing insights for similar bioprocess monitoring in the future.
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- Neutralizing monoclonal antibodies (nMABs) are effective in treating COVID-19, and researchers developed a new version called 55,315-VLVH to increase its potency.
- This new tetravalent IgG1-(scFv) construct combines two single chain variable fragments (scFv) with the original nMAB, improving its binding activity against spike protein variants and enhancing neutralization against variants of concern (VOCs).
- While the new version shows better antibody-dependent cellular phagocytosis (ADCP) without increasing inflammatory responses, it may lead to higher systemic inflammation through antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), requiring further research
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- A study was conducted on CHO cell fed-batch culture processes to improve biologics quality control, focusing on monitoring and identifying biochemical markers using 1H NMR and multivariate data analysis (MVDA).
- The researchers identified 63 metabolites in CHO cell-free supernatants and utilized multivariate statistical process control (MSPC) charts to confirm high quality consistency across batches, indicating a stable commercial-scale culture process.
- Biochemical markers were identified for different cell growth phases, and the study proposed a workflow that combines MVDA and 1H NMR to enhance understanding and monitoring of biomanufacturing processes for biologics.
The novel anti-PD-L1/TGFBR2-ECD fusion protein (BR102) comprises an anti-PD-L1 antibody (HS636) which is fused at the C terminus of the heavy chain to a TGF-β1 receptor Ⅱ ectodomain (TGFBR2-ECD), and which can sequester the PD-1/PD-L1 pathway and TGF-β bioactivity in the immunosuppressive tumor microenvironment. In the expression of TGFBR2-ECD wild-type fused protein (BR102-WT), a 50 kDa clipped species was confirmed to be induced by proteolytic cleavage at a "QKS" site located in the N-terminus of the ectodomain, which resulted in the formation of IgG-like clipping. The matrix metalloproteinase-9 was determined to be associated with BR102-WT digestion.
View Article and Find Full Text PDFCoronavirus disease 2019 (COVID-19) has become a worldwide threat to humans, and neutralizing antibodies have therapeutic potential. We have purified more than 1,000 memory B cells specific to SARS-CoV-2 S1 or its RBD (receptor binding domain) and obtain 729 paired heavy- and light-chain fragments. Among these, 178 antibodies test positive for antigen binding, and the majority of the top 17 binders with EC below 1 nM are RBD binders.
View Article and Find Full Text PDFSepsis is a serious disease that leads to severe inflammation, dysregulation of immune system, multi-organ failure and death. Innate response activator (IRA) B cells, which produce granulocyte-macrophage colony-stimulating factor (GM-CSF), protect against microbial sepsis. Lipid mediator lipoxin A4 (LXA4) exerts anti-inflammatory and immunoregulatory effects, and it has been reported that LXA4 receptor ALX/FPR2 is expressed on B cells.
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