Semiarbitrary qPCR for Sensitive Detection of Circulating miRNA via Terminal Deoxynucleotidyl Transferase-Assisted RNA-Primed DNA Polymerization.

Circulating microRNAs (miRNAs) have recently emerged as noninvasive disease biomarkers. Quantitative detection of circulating miRNAs could offer significant information for clinical diagnosis due to its significance in the development of biological processes. In response to the current challenges of circulating miRNA detection, we introduce a sensitive, selective, and versatile circulating miRNA detection strategy using terminal deoxynucleotidyl transferase (TdT)-catalyzed RNA-primed DNA polymerization (TCRDP) coupled with semiarbitrary qPCR (SAPCR).

Prevalence and Predictors of Hemorrhagic Foci on Long-term Follow-up MRI of Recent Single Subcortical Infarcts.

Hemorrhagic foci surrounding the lacune in the long-term evolution of recent single subcortical infarcts (RSSIs) remains largely unexplored. We aimed to determine the prevalence, characteristics, and predictors of hemorrhagic foci in patients with RSSI. From a prospective, longitudinal study of RSSIs, we recruited patients who underwent multimodal MRI assessments both at baseline and approximately one year after the stroke onset.

The Change in Fibrinogen is Associated with Outcome in Patients with Acute Ischemic Stroke Treated with Endovascular Thrombectomy.

Background: Fibrinogen has been identified as a modulator of the coagulation and inflammatory process. There is uncertainty about the relationship between the dynamic profile of fibrinogen levels and its impact on clinical outcomes in patients with acute ischemic stroke treated with endovascular thrombectomy.

Methods: We consecutively enrolled patients with acute ischemic stroke who underwent endovascular thrombectomy.

Impact Ionization Coefficient Prediction of a Lateral Power Device Using Deep Neural Network.

Nowadays, the impact ionization coefficient in the avalanche breakdown theory is obtained using 1-D PN junctions or SBDs, and is considered to be a constant determined by the material itself only. In this paper, the impact ionization coefficient of silicon in a 2D lateral power device is found to be no longer a constant, but instead a function of the 2D coupling effects. The impact ionization coefficient of silicon that considers the 2D depletion effects in real-world devices is proposed and extracted in this paper.

Experimental Study on the Rupture Behavior of the Liquid Bridge between Three Rigid Spheres.

The powder process is dynamic at the microscale level. Due to the capillary effect, the viscous attraction of the liquid phase between particles alters the strength and stability of wet granular materials. In this paper, experiments on the rupture behavior of the funicular liquid bridge between three rigid spheres have been presented.

WITHDRAWN: A deep learning framework for diagnosing periprosthetic joint infections using X-ray images: a discovery and validation study.

This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.

Self-Assembly of DNA Tiles with G-Quadruplex DNAzyme Catalytic Activity for Sensing Applications.

DNA tiles form through self-assembly of a small number of DNA strands that interact through basic repeated interactions, allowing the growth of nanoscale structures seeded by molecular inputs. If an approach for catalytic signal amplification can be integrated into the resultant nanostructure, then one can anticipate biosensing or diagnostic applications mediated by DNA tile self-assembly. Here, two-dimensional DNA tiles with split quadruplexes were designed as diagnostic tools for nucleic acid sensing without the use of protein enzymes.

Association of compromised cerebral perfusion with lenticulostriate artery impairments in the subacute phase of branch atheromatous disease.

Background Purpose: Whether altered cerebral perfusion is associated with the pathogenesis of single subcortical infarctions (SSIs) in the lenticulostriate artery (LSA) territory remains unclear.

Objective: We aimed to assess whether cerebral perfusion abnormalities are related to LSA impairments in the subacute phase of SSIs and then to examine their correlations with etiological subtypes of SSIs.

Methods: A total of 110 patients with acute SSIs in the LSA territory were prospectively recruited between July 2017 and October 2021, and they underwent magnetic resonance perfusion-weighted imaging (PWI) and whole-brain vessel-wall imaging (VWI) within 7 days of stroke onset.

COVID-19 impact on mental health.

Background: The coronavirus disease 2019 (COVID-19) pandemic has posed a significant influence on public mental health. Current efforts focus on alleviating the impacts of the disease on public health and the economy, with the psychological effects due to COVID-19 relatively ignored. In this research, we are interested in exploring the quantitative characterization of the pandemic impact on public mental health by studying an online survey dataset of the United States.

Targeting PSD95/nNOS by ZL006 alleviates social isolation-induced heightened attack behavior in mice.

Rationale: Deregulated attack behaviors have devastating social consequences; however, satisfactory clinical management for the behavior is still an unmet need so far. Social isolation (SI) has been common during the COVID-19 pandemic and may have detrimental effects on mental health, including eliciting heightened attack behavior.

Objectives: This study aims to explore whether injection of ZL006 can alleviate SI-induced escalation of attack behavior in mice.

Bactericidal efficiency and photochemical mechanisms of micro/nano bubble-enhanced visible light photocatalytic water disinfection.

Microbial contamination of water in the form of highly-resistant bacterial spores can cause a long-term risk of waterborne disease. Advanced photocatalysis has become an effective approach to inactivate bacterial spores due to its potential for efficient solar energy conversion alongside reduced formation of disinfection by-products. However, the overall efficiency of the process still requires significant improvements.

An integrated approach using ozone nanobubble and cyclodextrin inclusion complexation to enhance the removal of micropollutants.

Ozone (O) has been widely used for the elimination of recalcitrant micropollutants in aqueous environments, due to its strong oxidation ability. However, the utilization efficiency of O is constrained by its low solubility and short half-life during the treatment process. Herein, an integrated approach, using nanobubble technology and micro-environmental chemistry within cyclodextrin inclusion cavities, was studied in order to enhance the reactivity of ozonisation.

Numerical study on the adhesion of a circulating tumor cell in a curved microvessel.

The adhesion of a circulating tumor cell (CTC) in a three-dimensional curved microvessel was numerically investigated. Simulations were first performed to characterize the differences in the dynamics and adhesion of a CTC in the straight and curved vessels. After that, a parametric study was performed to investigate the effects of the applied driven force density f (or the flow Reynolds number Re) and the CTC membrane bending modulus K on the CTC adhesion.

Construction of a Novel Biosensor Based on the Self-assembly of Dual-Enzyme Cascade Amplification-Induced Copper Nanoparticles for Ultrasensitive Detection of MicroRNA153.

MicroRNAs (miRNAs) have received extensive attention because of their potential as biomarkers for cancer diagnosis and monitoring, and their effective detection is very significant. Here, a specific, one-pot, rapid, femtomolar sensitive miRNAs detection biosensor was developed based on the target-triggered three-way junction (3-WJ) and terminal deoxynucleotide transferase (TDT)/Nt.BspQI in combination with activated copper nanoparticles (CuNPs) self-assembly.

Nonenzyme Cascaded Amplification Biosensor Based on Effective Aggregation Luminescence Caused by Disintegration of Silver Nanoparticles.

Sensitive and portable quantification of biomarkers has particular significance in the monitoring and treatment of clinical diseases. Conventional immunoassays were accustomed to introducing or incorporating enzymes for signal amplification, which commonly suffered from poor stability and inferior tolerance. Herein, we constructed a novel nonenzyme amplification methodology based on fluorogenic Ag-tetrazolate aggregation coupled with silver corrosion sensitization for biomarker determination.

Numerical study on the dynamics of primary cilium in pulsatile flows by the immersed boundary-lattice Boltzmann method.

An explicit immersed boundary-lattice Boltzmann method is applied to numerically investigate the dynamics of primary cilium in pulsatile blood flows with two-way fluid-structure interaction considered. To well characterize the effect of cilium basal body on cilium dynamics, the cilium base is modeled as a nonlinear rotational spring attached to the cilium's basal end as proposed by Resnick (Biophys J 109:18-25, 2015. https://doi.

Revisiting spin-lattice relaxation time measurements for dilute spins in high-resolution solid-state NMR spectroscopy.

Numerous nuclear magnetic resonance (NMR) measurements of spin-lattice relaxation times (T1S) for dilute spins such as (13)C have led to investigations of the motional dynamics of individual functional groups in solid materials. In this work, we revisit the Solomon equations and analyze how the heteronuclear cross relaxation between the dilute S (e.g.

Controlled synthesis and tunable upconversion luminescence of NaYW2O8:Yb3+/Ho3+ nanocrystals.

Tetragonal phase NaYW2O8:Yb3+/Ho3+ nanocrystals were successfully synthesized using a hydrothermal method with a subsequent calcination treatment. Under 980 nm excitation, the 5S2/5F4 --> 5I8 and 5F5 --> 5I8 emissions were observed. The relative intensity of the 5F5 --> 5I8 to the 5S2/5F4 -->5I8 in the samples increased with increasing the pH values of the resultant solutions.

Distributed MLEM: an iterative tomographic image reconstruction algorithm for distributed memory architectures.

The processing speed for positron emission tomography (PET) image reconstruction has been greatly improved in recent years by simply dividing the workload to multiple processors of a graphics processing unit (GPU). However, if this strategy is generalized to a multi-GPU cluster, the processing speed does not improve linearly with the number of GPUs. This is because large data transfer is required between the GPUs after each iteration, effectively reducing the parallelism.

IntentSearch: Capturing User Intention for One-Click Internet Image Search.

Web-scale image search engines (e.g., Google image search, Bing image search) mostly rely on surrounding text features.

Fully 3D list-mode time-of-flight PET image reconstruction on GPUs using CUDA.

Purpose: List-mode processing is an efficient way of dealing with the sparse nature of positron emission tomography (PET) data sets and is the processing method of choice for time-of-flight (ToF) PET image reconstruction. However, the massive amount of computation involved in forward projection and backprojection limits the application of list-mode reconstruction in practice, and makes it challenging to incorporate accurate system modeling.

Methods: The authors present a novel formulation for computing line projection operations on graphics processing units (GPUs) using the compute unified device architecture (CUDA) framework, and apply the formulation to list-mode ordered-subsets expectation maximization (OSEM) image reconstruction.

Microsurgical treatment of cerebrospinal fluid rhinorrhea in sphenoidal sinus.

Objective: To explore the clinical manifestation, diagnosis and surgical treatment of cerebrospinal fluid rhinorrhea in sphenoidal sinus.

Methods: Nine cases of cerebrospinal fluid rhinorrhea in sphenoidal sinus from 2007 to 2009 were retrospectively analyzed consisting of their possible etiological factors, clinical manifestations, localization of the leakage site and treatment methods. Among them, there were 3 cases of traumatic rhinorrhea, 4 postoperative rhinorrhea and 2 spontaneous rhinorrhea.

Automated retrieval of CT images of liver lesions on the basis of image similarity: method and preliminary results.

Purpose: To develop a system to facilitate the retrieval of radiologic images that contain similar-appearing lesions and to perform a preliminary evaluation of this system with a database of computed tomographic (CT) images of the liver and an external standard of image similarity.

Materials And Methods: Institutional review board approval was obtained for retrospective analysis of deidentified patient images. Thereafter, 30 portal venous phase CT images of the liver exhibiting one of three types of liver lesions (13 cysts, seven hemangiomas, 10 metastases) were selected.