Publications by authors named "Jingshu He"

Article Synopsis
  • The hydrolysis step of 2,7-epoxy is crucial for the production of camptothecin (CPT), a compound with potent cancer-fighting properties, yet the specific genes involved have not been previously identified.
  • This study identifies three genes (CaEH1-CaEH3) responsible for the epoxide hydrolase activity in Camptotheca acuminata and highlights their ability to catalyze the opening of various oxirane rings.
  • Functional tests show that these genes are actively involved in CPT biosynthesis, are expressed in all plant tissues with a concentration in leaves, and suggest a convergence in their evolutionary development from different ancestral genes.
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Article Synopsis
  • - Recent research has clarified the chemical process for flavonol production in the plant Camptotheca acuminata, but the specific genes responsible for its methylation are still not fully understood.
  • - Two important genes, CaFOMT1 and CaFOMT2, were identified as key players in this process, with CaFOMT1 specifically methylating the 4'-OH group of quercetin and CaFOMT2 acting on both the 4'-OH and 7-OH groups.
  • - Analysis of their molecular structure revealed specific sites essential for their function, while also indicating that these genes evolved separately from a different lineage than previously identified methyltransferases.
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  • A new QTL for adult-plant stripe rust resistance was found in cultivated emmer wheat, specifically located on wheat chromosome 2BL.
  • This research involved mapping and characterizing resistance derived from emmer wheat accession AS286 using a specific genotyping method and testing against various prevalent rust races.
  • The study identified multiple QTLs with significant impacts on stripe rust resistance, particularly QYrAS286-2BL, which is unique for only showing resistance at the adult-plant stage and could aid in breeding programs through marker-assisted selection.
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The NAC transcription factor (TF) family is one of the largest TF families in plants, which has been widely reported in rice, maize and common wheat. However, the significance of the NAC TF family in wild emmer wheat ( ssp. ) is not yet well understood.

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The zinc/iron-regulated transporter-like protein (ZIP) family has a crucial role in Zn homeostasis of plants. Although the ZIP genes have been systematically studied in many plant species, the significance of this family in wild emmer wheat ( ssp. ) is not yet well understood.

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Two advanced wheat lines BAd7-209 and BAd23-1 without the functional gene were obtained from a cross between common wheat cultivar Chuannong 16 (CN16) and wild emmer wheat accession D97 (D97). BAd7-209 showed superior quality parameters than those of BAd23-1 and CN16. We found that the components of glutenins and gliadins in BAd7-209 and BAd23-1 were similar, whereas BAd7-209 had higher amount of glutenins and gliadins than those of BAd23-1.

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Stripe rust, caused by f. sp. , is a damaging disease of wheat globally, and breeding resistant cultivars is the best control strategy.

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Article Synopsis
  • Stripe rust, caused by the fungus Puccinia striiformis f. sp. tritici (Pst), is a significant global threat to wheat production, and breeding resistant wheat cultivars like Shumai126 (SM126) presents an effective control strategy.
  • The study utilized RNA-seq to examine the transcriptomic changes in SM126 during Pst infection across different time points, identifying a total of 2,107 differentially expressed genes (DEGs) at 1, 3, and 7 days post-inoculation, with most DEGs showing transient expression patterns.
  • Analysis revealed key biological processes and pathways involved in SM126's response to the pathogen, highlighting the potential of identified genes, including R proteins
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The grain protein content (GPC) in modern wheat is inherently low. Wild emmer wheat (Triticum turgidum ssp. dicoccoides, 2n = 4x = 28, AABB) gene pool harbors wide genotypic variations in GPC.

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Two advanced lines (BAd7-209 and BAd7-213) with identical high-molecular-weight glutenin subunit composition were obtained via wide hybridization between low-gluten cultivar chuannong16 (CN16) and wild emmer D97 (D97). BAd7-209 was better than BAd7-213, and both of them were much better than CN16 in a dough quality test. We found that BAd7-209 had more abundant and higher expression levels of low-molecular-weight glutenin subunit (LMW-GS) proteins than those of BAd7-213.

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We report the characterization of seven novel low-molecular-weight glutenin subunit (LMW-GS) genes from (2 = 2 = 14, MM). We found that all seven LMW-GS genes possessed the same primary structure shared by other known LMW-GSs. Three genes (, and ) encode LMW-m-type subunits, two ( and ) encode LWM-i-type subunits, and two ( and ) encode LWM-l-type subunits.

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