A robust SERS calibration using a pseudo-internal intensity reference.

Surface-enhanced Raman scattering (SERS) with high molecular sensitivity and specificity is a powerful nondestructive analytical tool. Since its discovery, SERS measurements have suffered from the vulnerability of calibration curve, which makes quantification analysis a great challenge. In this work, we report a robust calibration method by introducing a referenced measurement as the intensity standard.

Controlling the Morphologies of Silver Aggregates by Laser-Induced Synthesis for Optimal SERS Detection.

Controlling the synthesis of metallic nanostructures for high quality surface-enhanced Raman scattering (SERS) materials has long been a central task of nanoscience and nanotechnology. In this work, silver aggregates with different surface morphologies were controllably synthesized on a glass-solution interface via a facile laser-induced reduction method. By correlating the surface morphologies with their SERS abilities, optimal parameters (laser power and irradiation time) for SERS aggregates synthesis were obtained.

[The effect of aluminum adjuvant and immunization schedule on immunogenicity of Sabin inactivated poliovirus vaccine].

Objective: To study the effect of aluminume adjuvant and immunization schedule on immunogenicity of Sabin inactivated poliovirus vaccine.

Methods: Four batches of Sabin IPV were produced by different concentrations of type 1, 2, and 3 poliovirus and administrated on three-dose schedule at 0, 1, 2 months and 0, 2, 4 months on rats. Serum samples were collected one month after each dose and neutralizing antibody titers against three types poliovirus were determined by micro-neutralization assay.

[Prokaryotic soluble expression of protein D of Haemophilus influenzae type b].

Objective: To express the recombinant D protein in prokaryotic expression system solubly and make preparation for producing D-carrier conjugate vaccine next step.

Methods: The hpd gene fragment removed of signal peptide from genomic DNA of Hib CMCC was inserted into pET43. 1a.

FgTep1p is linked to the phosphatidylinositol-3 kinase signalling pathway and plays a role in the virulence of Fusarium graminearum on wheat.

Both mammalian tensin-like phosphatase 1 [TEP1; also known as phosphatase deleted on chromosome 10 (PTEN) or mutated in multiple advanced cancer 1 (MMAC1)] and Saccharomyces cerevisiae ScTep1p are involved in the phosphatidylinositol pathway. In this study, we identified the Fusarium graminearum locus FGSG_04982.3 (named FgTEP1) as the functional homologue of ScTEP1 in the sensitivity of S.

The type 2C protein phosphatase FgPtc1p of the plant fungal pathogen Fusarium graminearum is involved in lithium toxicity and virulence.

Type 2C protein phosphatases (PP2Cs) are monomeric protein serine/threonine phosphatases that play various roles in eukaryotic organisms. In this study, we characterized the PP2C encoded by FgPTC1 in Fusarium graminearum, the major causal agent of Fusarium head blight on wheat and barley. We found that deletion of FgPTC1 delays the mycelium growth of F.