Antipathogenic Activities of Volatile Organic Compounds Produced by LT1 against LC1, the Pathogen of Southern Blight in .

This study explores the antipathogenic properties of volatile organic compounds (VOCs) produced by LT1, isolated from the rhizosphere soil of . The impact of these VOCs on the mycelial growth of LC1, the causative agent of southern blight in , was evaluated using a double Petri-dish assay. The biocontrol efficacy of these VOCs was further assessed through leaf inoculation and pot experiments.

LT1: a potential biocontrol agent for southern blight on .

Introduction: Southern blight, caused by , poses a serious threat to the cultivation of , a plant with significant medicinal value. The overreliance on fungicides for controlling this pathogen has led to environmental concerns and resistance issues. There is an urgent need for alternative, sustainable disease management strategies.

De Novo RNA Sequencing and Transcriptome Analysis of Gene Expression during Sclerotium Development.

is a destructive soil-borne fungal pathogen that causes stem rot in cultivated plants. However, little is known about the genetic basis of sclerotium development. In this study, we conducted de novo sequencing of genes from three different stages of (mycelia, early sclerotium formation, and late sclerotium formation) using Illumina HiSeq 4000.

Leaf Spot Caused by Didymella glomerata on Chaihu (Bupleurum falcatum L.) in China.

Bupleurum falcatum is a Apiaceae family herbal medicinal plant, which has the functions of soothing liver, relieving depression, relieving fever, dispelling stagnation, and regulating menstruation. B. falcatum roots have been used in Chinese herbal formbulary for at least 2000 years (Ahmadimoghaddam et al.

Development of EST-SSR primers and genetic diversity analysis of the southern blight pathogen using transcriptome data.

Introduction: . is a globally dispersed pathogenic fungus that causes southern blight disease in many crops and Chinese herbal medicine. The high degree of variation and diversity in the fungi altered population genetic structure.

Franch root rot infection disrupts microecological balance of rhizosphere soil and endophytic microbiomes.

Introduction: The ecological balance of the plant microbiome, as a barrier against pathogens, is very important for host health. is one of the important medicinal plants in China. In recent years, Illumina Miseq high-throughput sequencing technology was frequently used to analyze root rot pathogens and the effects of root rot on rhizosphere microorganisms of .

BcCDI1 protein triggers both plant cell death and immune response.

Cell death-inducing proteins (CDIPs) play important roles in the infection of , a broad host-range necrotrophic phytopathogen. Here, we show that the secreted protein BcCDI1 (Cell Death Inducing 1) can cause necrosis in tobacco leaves and at the same time elicit plant defense. The transcription of was induced at the infection stage.

Transcriptomic and metabolomic analyses provide new insights into the appropriate harvest period in regenerated bulbs of .

Introduction: The bulb of , a traditional cough and expectorant medicine, is usually harvested from June to September according to traditional cultivation experience, without practical scientific guidance. Although steroidal alkaloid metabolites have been identified in , the dynamic changes in their levels during bulb development and their molecular regulatory mechanisms are poorly understood.

Methods: In this study, integrative analyses of the bulbus phenotype, bioactive chemical investigations, and metabolome and transcriptome profiles were performed to systematically explore the variations in steroidal alkaloid metabolite levels and identify the genes modulating their accumulation and the corresponding regulatory mechanisms.

Transcriptomic and metabolic analyses reveal the potential mechanism of increasing steroidal alkaloids in through intercropping with .

, a well-known medicinal perennial herb, is used as an antitussive and an expectorant. Continuous cropping and monoculture cultivation usually negativly affect the growth of . Compared with the monoculture system, the - intercropping system significantly increases the yield of .

Full-Length Transcriptomic Sequencing and Temporal Transcriptome Expression Profiling Analyses Offer Insights into Terpenoid Biosynthesis in .

Folium is a traditional herbal medicine used for moxibustion heat therapy in China. The volatile oils in leaves are closely related to its medicinal value. Records suggest that the levels of these terpenoids components within the leaves vary as a function of harvest time, with June being the optimal time for harvesting, owing to the high levels of active ingredients during this month.

First Report of Anthracnose Caused by on Goldthread in China.

Goldthread ( Franch) is one of the most widely used Chinese traditional medicine plants with remarkable medicinal properties (Mizrahi et al. 2014). In July 2019, a new anthracnose-like leaf spot disease was observed in Banqiao Town, Enshi, Hubei, China.

Pseudomonas cichorii causing leaf spot disease on Banxia (Pinellia ternata) in China.

Banxia (Pinellia ternata) is an important Chinese medicinal material in the family Araceae and is a widely grown herb in China. In September 2021, a leaf spot disease was observed on Banxia field, with an incidence rate of 35 to 40 % in a 4-ha field, in Zhongxiang City, Hubei Province of China. Symptoms were observed as yellow-white centers, water-soaked edges, irregular lesions, and gradually developed into a yellowish-brown center and a dark-brown edge.

[Identification, biological characteristics, and control of pathogen causing Pinellia ternata soft rot in Hubei province].

This study was designed to identify the pathogen causing soft rot of Pinellia ternata in Qianjiang of Hubei province and screen out the effective bactericides, so as to provide a theoretical basis for the control of soft rot of P. ternata. In this study, the pathogen was identified based on molecular biology and physiological biochemistry, followed by the detection of pathogenicity and pathogenicity spectrum via plant tissue inoculation in vitro and the indoor toxicity determination using the inhibition zone method to screen out bactericide with good antibacterial effects.

Baseline Sensitivity and Control Efficacy of Strobilurin Fungicide Pyraclostrobin Against .

is a fungi pathogen of southern blight with broad host range. The quinone outside inhibitor fungicide pyraclostrobin was officially approved for controlling many diseases in 2015. In this study, baseline sensitivity of to pyraclostrobin was established by measuring the 50% effective concentration (EC) values of 155 isolates of .

Duohua huangjing (Polygonatum cyrtonema Hua) seedling basal rot caused by Fusarium redolens in China.

Duohua huangjing (Polygonatum cyrtonema Hua) seedling basal stem rot caused by Fusarium redolens in China Tao Tang1, Fanfan Wang1, Jie Guo1, Xiaoliang Guo1, Yuanyuan Duan1,Jingmao You1* 1 Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi, 445000, China. Duohua huangjing (Polygonatum cyrtonema Hua), a herbal medicine, that is mostly planted in several provinces in China. In April 2020, severe diseases with about 40% seedling losse was found in the Huangjing seedling base in Shiyan city, Hubei province.

Occurrence of Dickeya fangzhongdai Causing Soft Rot of Banxia (Pinellia ternata) in China.

Banxia [Pinellia ternata (Thunb.) Breit., Araceae] is a perennial herbaceous plant, where the tuber is commonly used in traditional Chinese herbal medicine.

Occurrence of Stem Blight Caused by Pseudomonas extremorientalis on Pinellia ternata in China.

Pinellia ternata is a perennial herbaceous plant, which tubers can be used for anti-inflammatory and has a significant position in Traditional Chinese Medicine (Marki et al. 1987). In April 2020, bacterial stem blight first occurred on P.

Fusarium acuminatum Associated with Root Rot of Ophiopogon japonicus (Linn. f.) in China.

Ophiopogon japonicus (Linn. f.) is a perennial evergreen in the Liliaceae family that is cultivated in many provinces of China due to its high medicinal and economic value .

First report of Sclerotinia blight caused by Sclerotinia sclerotiorum on Pinellia ternata in China.

Pinellia ternata is a perennial herb that belongs to the Araceae Family. The tuber of the P. ternata plant contains active ingredients such as alkaloids and pinellia starch, and can be used as a Chinese medicine for antineoplastic and anti-inflammatory disorders in humans (Marki et al.

[Effects of biocontrol strain BZJN1 and streptoprofen on physicochemical properties and bacteria structure of rhizosphere soil of Atractylodes macrocephala].

Soil microorganisms are one of the important biological indictors of soil quality and can reflct the comprehensive ecological environment characteristics of the soil. The research of soil microbial diversity is the key to know the ecological functions and balance with soil. In this paper, high-throughput sequencing on PCR-amplified 16 S rRNA gene V3-V4 fragments was used to determine the bacterial diversity in rhizosphere soil of A.

Full-length transcriptome sequencing and modular organization analysis of oleanolic acid- and dammarane-type saponins related gene expression patterns in Panax japonicus.

The saponins found in Panax japonicus, a traditional medicinal herb in Asia, exhibit high degrees of structural and functional similarity. In this study, metabolite analysis revealed that oleanolic acid-type and dammarane-type saponins were distributed unevenly in three tissues (rhizome_Y, rhizome_O, and secRoot) of P. japonicus.

[Isolation and identification of pathogen of Dendrobium officinale gray mold and its prevention and control].

Through investigation,it was found that the main disease of leaves was grey mold on Dendrobium officinale in Hubei province,which has a great impact on the yield and quality of D. officinale. The identification of morphological and molecular biological was used to prove that the pathogen was Botrytis cinerea.